Post AaUQ4GDdhnb4yWfI00 by gringene@genomic.social
 (DIR) More posts by gringene@genomic.social
 (DIR) Post #AaUQ4EUw6WFDdak4gK by gringene@genomic.social
       2023-09-23T23:55:15Z
       
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       @nanopore It seems the reason I'm getting confused about the precise sequences in the TrackIt 1kb Plus DNA ladder is that there are multiple different sequences that fit into the same band length.Either that, or they're fragments of longer bands that have been clipped at very specific lengths.#DNAladder #Flongle #LSK114
       
 (DIR) Post #AaUQ4FSqVmAOdOlv3w by gringene@genomic.social
       2023-09-24T12:51:54Z
       
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       @nanopore I'm up to 650bp now.Despite the longest sequence so far having a reported BLAST identity of 98%, I've double-checked the reads, and decided that it's more likely that there's variation from the closest matching reference than a sequencing error.#DNAladder
       
 (DIR) Post #AaUQ4GDdhnb4yWfI00 by gringene@genomic.social
       2023-10-06T09:23:05Z
       
       0 likes, 1 repeats
       
       @nanopore 1kb seems to be about the limit of what I can pick out easily with LSK114 + Flongle.But I think that's probably okay, because from memory the longer ones were easier to work out / assemble from RBK-fragmented reads.Then again.... maybe I should just go all out and use a PromethION flow cell with LSK114.
       
 (DIR) Post #AaUQ4JdczdATZzqaQK by gringene@genomic.social
       2023-09-24T13:40:24Z
       
       0 likes, 0 repeats
       
       Note: sequences are subject to change without notice. In particular, some appear to be perfect subsequences of others, so I need to hunt for better ones.