Posts by gringene@genomic.social
(DIR) Post #ASai6xkxRzQFT2WkGe by gringene@genomic.social
2023-02-12T02:57:25Z
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@escarpment @futurebird what I'm saying in a roundabout way is that we have a single origin in part because the software we use to generate visualisations assumes a single origin.
(DIR) Post #ASai6ymPe4BEdqDQAq by gringene@genomic.social
2023-02-12T03:08:12Z
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@escarpment @futurebird no, not a metaphor at all.Look at any phylogenetic tree in any publication. They're pretty much all outward branching with no horizontal transfer.Even for bacteria, which we know share genes around more than... a really sharey thing.https://en.wikipedia.org/wiki/Phylogenetic_tree
(DIR) Post #ASai6zqhfbCrxREMV6 by gringene@genomic.social
2023-02-12T03:22:22Z
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@escarpment @futurebird yes, as far as we know, DNA is the only thing that creates life, as we understand it.However...1. There is tentative link to the creation of a nucleic acid or amino acid world from "nothing", so it's not completely out of the realm of possibility to suggest that there were multiple origins that were spliced together.2. Nature has other non-DNA ways to replicate (fire is my favourite example), but we don't treat the things that replicate in a non-DNA as being alive.
(DIR) Post #ASnhWs4hcfggTb22Bk by gringene@genomic.social
2023-02-18T10:08:47Z
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@mjg59 so in other words, SMS 2FA is better than FA 2FA
(DIR) Post #AUbUt88lMGOHfFbfhA by gringene@genomic.social
2023-04-13T08:35:00Z
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@mjg59 yes, but it might alter your code in the process.
(DIR) Post #AYoEXpYgABwgR09aEq by gringene@genomic.social
2023-08-17T00:30:21Z
2 likes, 2 repeats
#3DPrinting New #BrailleKeycaps from #Elecrow have just arrived!This is quite a big improvement from my last batch. I might add a bit of nail polish to the braille bumps to accentuate them.
(DIR) Post #AaUQ4EUw6WFDdak4gK by gringene@genomic.social
2023-09-23T23:55:15Z
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@nanopore It seems the reason I'm getting confused about the precise sequences in the TrackIt 1kb Plus DNA ladder is that there are multiple different sequences that fit into the same band length.Either that, or they're fragments of longer bands that have been clipped at very specific lengths.#DNAladder #Flongle #LSK114
(DIR) Post #AaUQ4FSqVmAOdOlv3w by gringene@genomic.social
2023-09-24T12:51:54Z
0 likes, 0 repeats
@nanopore I'm up to 650bp now.Despite the longest sequence so far having a reported BLAST identity of 98%, I've double-checked the reads, and decided that it's more likely that there's variation from the closest matching reference than a sequencing error.#DNAladder
(DIR) Post #AaUQ4GDdhnb4yWfI00 by gringene@genomic.social
2023-10-06T09:23:05Z
0 likes, 1 repeats
@nanopore 1kb seems to be about the limit of what I can pick out easily with LSK114 + Flongle.But I think that's probably okay, because from memory the longer ones were easier to work out / assemble from RBK-fragmented reads.Then again.... maybe I should just go all out and use a PromethION flow cell with LSK114.
(DIR) Post #AbAGK4h97Z6DqLpZSq by gringene@genomic.social
2023-10-26T07:51:27Z
0 likes, 1 repeats
Shifting and re-coiling the wire from the old stator to the new stator was a pleasant experience.A++, would trade again! 😊#3BladedUgrinsky #3DPrinting
(DIR) Post #AjwzNkIYLtMLZeS8ky by gringene@genomic.social
2024-07-15T07:02:40Z
0 likes, 1 repeats
I've been made redundant from my job, effective tomorrow.So... if you know anyone who's in need of a bioinformatician to work remotely on small bioinformatics projects, please let me know.