Newsgroups: bionet.molbio.methds-reagnts
Path: utzoo!utgpu!news-server.csri.toronto.edu!rpi!zaphod.mps.ohio-state.edu!magnus.acs.ohio-state.edu!news
From: webener@hpuxa.ircc.ohio-state.edu (William C. Ebener)
Subject: Lambda cloning
Message-ID: <1991Apr20.152329.4870@magnus.acs.ohio-state.edu>
Originator: webener@hpuxa.acs.ohio-state.edu
Sender: news@magnus.acs.ohio-state.edu
Nntp-Posting-Host: hpuxa.acs.ohio-state.edu
Organization: The Ohio State University
Date: Sat, 20 Apr 1991 15:23:29 GMT
Lines: 11

Help.

	I want to make several size selected genomic carrot libraries using
Charon 21A to help me pull out the genomic equivalent of a
particular cDNA.  One library will contain HindIII fragments from 2.0 to
4.5 kb and the other library will be an EcoRI from 3.0 to 9.0 kb.  Any
suggestions about my choice of vector and/or where I might be able to
obtain Charon 21A (perhaps as phosphotased arms) would be helpful.
Thanks.
-- 
--- Later, EB  
