[HN Gopher] I Managed to Grow Countable Yeast Colonies
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       I Managed to Grow Countable Yeast Colonies
        
       Author : crescit_eundo
       Score  : 18 points
       Date   : 2025-10-14 20:36 UTC (9 days ago)
        
 (HTM) web link (chillphysicsenjoyer.substack.com)
 (TXT) w3m dump (chillphysicsenjoyer.substack.com)
        
       | rbartelme wrote:
       | Countable is a relative term in microbiology. I like that the
       | author stuck to the phrase "countable colonies", since colony
       | forming units are not really "countable as cells".
       | 
       | Allan Konopka does a good deep dive into "The Great Plate Count
       | Anomaly" here: https://thinkmicrobe.substack.com/p/the-great-
       | plate-count-an...
        
       | koeng wrote:
       | Very chill :)
       | 
       | I do this at an industrial scale. It gets really annoying as you
       | scale up to hundreds / thousands of different strains, all of
       | which need pickable colonies.
       | 
       | A serial dilution 3 or 4 times seems to always do the trick.
       | Typically on a robotic workstation you have to aspirate 6.5uL,
       | then slowly dispense 5.5uL above the Petri dish (sbs format) and
       | then stab into the agarose. Makes lovely perfectly-sized and
       | separated wells, so 96 cell lines fit on only 3 or 4 plates.
       | 
       | With better plate reading you can get that down to 1 or 2 plates
       | but it's less reliable
        
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