[HN Gopher] Biological Miracle - Wood Frog
       ___________________________________________________________________
        
       Biological Miracle - Wood Frog
        
       Author : thunderbong
       Score  : 474 points
       Date   : 2024-11-15 18:16 UTC (1 days ago)
        
 (HTM) web link (www.nps.gov)
 (TXT) w3m dump (www.nps.gov)
        
       | pwdiscflatmajor wrote:
       | I now know why I require so much sugar and sweets: I am a wood
       | frog preparing for hibernation!
        
       | sudobash1 wrote:
       | There are no pictures of the frogs in this article. For pictures
       | (of thawed and frozen frogs) you can see:
       | https://shakerlakes.org/frozen-frogs/
        
         | olejorgenb wrote:
         | From the linked video: timelapse of the thawing process:
         | https://youtu.be/pLPeehsXAr4?t=176
        
         | chasil wrote:
         | They also have a wiki, but not many photos. One photo is from
         | Quebec.
         | 
         | The wiki also mentions that urea is produced, in addition to
         | glucose, and both act as cryoprotectants.
         | 
         | https://en.m.wikipedia.org/wiki/Wood_frog
        
         | nuancebydefault wrote:
         | Looks like a normal (frozen) frog to me
        
       | moralestapia wrote:
       | Fascinating phenomena, thanks a lot for sharing!
        
       | metalman wrote:
       | cant even call that hibernation. There are other knock on
       | advantages to bieng frozen solid, it would slow down and even
       | kill a lot of infectious microbes.It may convey a certain life
       | extension benifit. And threre is not much behavioral adaptation
       | needed,frog gets chilly, snuggles under a leaf,freezes solid,
       | gets defrosted 8 months later and wakes up hungry and horny, not
       | bad.
        
         | duskwuff wrote:
         | > it would slow down and even kill a lot of infectious microbes
         | 
         | I wouldn't count on it killing them. The cryoprotectants in the
         | frog's body don't discriminate; they'll protect foreign
         | bacteria just as well as the frog's cells.
        
           | metalman wrote:
           | I should of elaborated, many of the issues associated
           | with,"conventional hibernation" have to do with lethal
           | infections aquired externaly while hibernating, damp, cold,
           | ....mold as to the cryoprotectants ,side protecting microbes,
           | clearly gut microbes and other internal flora ,would
           | benifit......but ,big but, would a sneeky cryosuspension
           | routine also include a freeze and clense cycle?, why not! and
           | easy enough to verify,right!
        
             | seesaw wrote:
             | I wonder what happens to the gut bacteria ? Do they also
             | freeze and get thawed ? If so, are those bacterium also
             | having a special adaptation, or is it a function of the
             | host ?
        
           | m463 wrote:
           | I would imagine the frog's internal ecosystem has co-evolved
           | with lots of bacteria that can also be frozen solid.
        
         | flir wrote:
         | > It may convey a certain life extension benefit.
         | 
         | I think it would be more interesting if it _doesn 't_ affect
         | lifespan. It would be a really counter-intuitive result (to
         | me).
        
           | metalman wrote:
           | either way, or any info that could be gathered about life
           | expectancy due to cryosuspension hibernation, is good, even a
           | null result is data that plays into many conversations about
           | longevity, space travel, temporary medical suspension to buy
           | time. The fact of this type of biological process, moves the
           | brackets quite a bit in how to think about many? all? other
           | biological processes. Here is a little game....try and think
           | about the world from a wood frogs perspective
        
       | killjoywashere wrote:
       | Evolutionarily, amphibians are somewhat simpler than mammals,
       | they're smaller than a lot of mammals and they don't live as
       | long, so I suspect some of this is simply that "things that
       | aren't there". They wouldn't have as many problems with advanced
       | glycation end products because the temperature is so low. There's
       | at least one other ice survival strategy: antifreeze proteins.
       | The fir tree and a variety of arctic fish have these:
       | https://pmc.ncbi.nlm.nih.gov/articles/PMC6691018/.
       | 
       | In all cases, I still don't understand how the membrane
       | potentials are maintained or re-constructed in the thawing phase:
       | any pointers?
        
         | monkeycantype wrote:
         | Perhaps because ice forms outside cells, freezing ions in
         | place. Even if there was a very small area around ion channels
         | that was liquid that equalized all ion concentrations with the
         | cell, when the extra cellular fluid thaws the original
         | concentrations would be pretty much restored
        
         | danwills wrote:
         | I think there are threads in Michael Levin's work that suggest
         | membrane voltage potential may be able to be 'encoded' to
         | microtubule structures and converted back into voltage
         | potentials later. I don't think it's firmly established or
         | understood yet but seems like a promising area for research!
        
       | pkoird wrote:
       | Curious to how long the frozen structure can "survive". I wonder
       | if it's a good idea to freeze one such frog and thaw it centuries
       | later (an amphibian time-traveler!)
        
         | olejorgenb wrote:
         | - Artificial experiment [1]: no longer than 3 months (but see
         | disclaimer)
         | 
         | - New study [2]: 7 months (with 100% survival rate)
         | 
         | So further study seems to be needed.
         | 
         | [1]
         | https://journals.biologists.com/jeb/article/216/18/3461/1160...
         | 
         | [2]
         | https://journals.biologists.com/jeb/article/217/12/2193/1211...
        
           | crazygringo wrote:
           | If kept in pristine conditions (perfectly sealed to prevent
           | evaporation leading to dehydration, deep freeze), is there a
           | particular chemical needed for life that we might expect to
           | break down first?
           | 
           | Since chemical reactions happen in the freezer just slower...
        
             | Qem wrote:
             | > Since chemical reactions happen in the freezer just
             | slower.
             | 
             | Can't the gross just be kept at artificially lie
             | temperatures to lengthen the stasis time? Say, -100C or
             | even lower?
        
               | Qem wrote:
               | *the frog
        
               | maronato wrote:
               | The article says their cells don't freeze during
               | hibernation. -100C would probably freeze the cells and
               | kill them
        
         | muzani wrote:
         | It's a bit of a fantasy that we might find a perfectly well
         | preserved dinosaur in a glacier somewhere.
        
           | t-3 wrote:
           | Not possible - the oldest ice we've found on the planet is
           | much less than 10 million years old. Even if some area
           | remained frozen long enough to contain an intact dinosaur, it
           | would be buried deeply enough and under enough pressure to
           | just be a bit more arctic oil by now.
        
             | 8n4vidtmkvmk wrote:
             | What about amber? Don't tell me that's a lie too
        
           | ComputerGuru wrote:
           | Forget the dinosaurs, we can't even preserve the glaciers
           | themselves these days!
        
       | megiddo wrote:
       | Not one photo of said frog.
       | 
       | Look, new rule: you write an article about frogs, you include a
       | photo.
        
         | jjcm wrote:
         | here's an illustration of one:
         | https://image.non.io/e9078e7d-5eb4-4787-9fe2-d7068d01a4df.we...
        
           | daredoes wrote:
           | this was really helpful! thanks!
        
         | sgt wrote:
         | But the article explains perfectly how they look like. White
         | eyes, glucose filled cells, frozen solid as a rock. Bang it
         | against a table, it's definitely frozen. Throw it back
         | underneath those leaves.
        
         | speerer wrote:
         | Like the Tree Octopus?
        
       | soco wrote:
       | I really expected a wood(en) frog here, qualifying truly as a
       | biological miracle. But the wood frogs are cool as well.
        
         | furyofantares wrote:
         | Oh god. This made me wonder, what _is_ wood, anyway? And I 've
         | just come away much more confused.
         | 
         | Bamboo is a grass and doesn't come from a tree. Palm wood comes
         | from palm trees, except palm tree trunks are apparently a
         | totally different type of structure than other tree trunks,
         | sounds closer to Papyrus. No growth rings, a fiber type
         | structure. Is Papyrus wood?
         | 
         | Any plant matter above a certain density? I don't think that's
         | it. Corn stalks aren't wood.
         | 
         | Man, I don't know. I am certain that it must be plant matter
         | though, so yes, a wooden frog would be a biological miracle.
        
           | yial wrote:
           | Wood is secondary xylem produced by growth from the vascular
           | Cambium. (Sometimes? I think the issue is that there's more
           | than one definition of wood depending on context ...?).
           | Growth rings?
           | 
           | But palm trees aren't truly trees, right ? Just called
           | trees.... They're more of a tree like shrub? I think.
        
             | furyofantares wrote:
             | Trying to learn about this through claude was kinda funny.
             | 
             | Ask it to tell me about wood that doesn't come from trees,
             | and it tells me about palm wood. I say, but doesn't that
             | come from palm trees? It says palm trees aren't technically
             | trees because their trunk isn't wooden.
             | 
             | Anyway, with your definition palm wood wouldn't be wood,
             | and neither would bamboo. Feels like the vegetable/fruit
             | thing though, there just isn't a perfect answer.
        
           | mawise wrote:
           | It's worse than that. _Tree_ isn't even a well-defined thing.
           | 
           | > Trees are not a monophyletic taxonomic group but consist of
           | a wide variety of plant species that have independently
           | evolved a trunk and branches as a way to tower above other
           | plants to compete for sunlight.[1]
           | 
           | [1]: https://en.wikipedia.org/wiki/Tree
        
           | mkl wrote:
           | I don't know a precise definition, but wood has many origins.
           | Wood has evolved hundreds of separate times, including at
           | least 38 separate times just on the Canary Islands. https://w
           | ww.researchgate.net/publication/341287935_Multiple_..., https
           | ://www.pnas.org/doi/pdf/10.1073/pnas.2208629119?downloa...
        
           | RajT88 wrote:
           | What's really going to bake your noodle later is that Corn is
           | a grass, just like Bamboo.
           | 
           | My total armchair answer: It helps to think about trees as
           | just giant shrubs.
           | 
           | Shrubs are considered "woody", but most definitely are not
           | trees. There are plenty of trees which are close relatives of
           | shrubs (like poison oak and the urushi tree).
           | 
           | So what's the difference between a grass and a tree? Walking
           | the tree of life up from Poison Oak and Bamboo, we see we
           | land at Monocotyledon and Eudicots. There's lots of non-woody
           | and tough fibrous (i.e. woody) plans in both clades (palm
           | trees are monocots, btw).
           | 
           | Wikipedia says if it is tough and fibrous and has growth
           | rings it's wood:
           | 
           | https://en.wikipedia.org/wiki/Wood
           | 
           | So Bamboo, although coming from a grass and not a tree, is
           | wood. Further reading down that page talks about density as a
           | key quality of wood, and goes on to _not definitively quality
           | bamboo as wood or as non-wood_ but some are dense enough.
           | 
           | Ultimately, there's no super clean definition of wood is my
           | take-away, between the technical and colloquial aspects. You
           | can use bamboo in construction much like wood, if you cut off
           | a bit of shrub and dry it out, it's a "stick" just as much as
           | if you trimmed it off a tree. You can make paper out of all
           | kinds of fibers.
        
           | NoImmatureAdHom wrote:
           | It's best to think of it as "tree-ing" or "to tree"
           | 
           | A really, really fantastic article:
           | https://eukaryotewritesblog.com/2021/05/02/theres-no-such-
           | th...
        
       | the_arun wrote:
       | Nicely written article. A few pictures could have made it more
       | interesting.
        
       | epicureanideal wrote:
       | Someone tell Alcor about this.
        
       | dexwiz wrote:
       | Wood frogs only live 3-5 years, so they probably only go through
       | a max of 5 of these cycles. I wonder how much cellular damage
       | they accumulate during these cycles that they can tolerate due to
       | short lifespans. They also have ~10,000x less neurons than a
       | mammal.
       | 
       | Even if you had the biochemistry that was able to do this, how
       | many cycles could a higher life form tolerate this, assuming it
       | would even work? Complex life seems to sacrifice some resiliency,
       | such as the ability to regrow limbs. Amphibians already seem to
       | be particularly adept at regeneration.
        
         | e44858 wrote:
         | "frogs don't freeze once and stay frozen. Instead, they spend a
         | week or two freezing at night and thawing during the day until
         | the temperatures drop permanently below freezing"
         | 
         | https://shakerlakes.org/frozen-frogs/
        
           | MPSimmons wrote:
           | like maple syrup...
        
             | kortilla wrote:
             | Or water... or anything else with a freezing point that the
             | ambient temperature crosses at night
        
               | MPSimmons wrote:
               | Oh sorry, I more meant specific to the times when you
               | harvest maple syrup.
               | 
               | If you didn't know, you need to wait to harvest maple
               | syrup until you have a series of days when the daytime
               | temperature is above freezing and the nighttime
               | temperature is below freezing. This causes the maple sap
               | to flow and allows it to collect in the bucket.
               | 
               | https://botanistinthekitchen.blog/2013/03/18/maple-syrup-
               | mec...
        
         | 8n4vidtmkvmk wrote:
         | I don't understand why we're not allowed to regrow limbs. What
         | did that make room for in our DNA?
        
           | macrolime wrote:
           | Some animals regenerate, some form scar tissue. It's thought
           | that scar tissue is a cheaper and faster mechanism than
           | regrowing limbs and has thus been selected in many cases.
        
             | 8n4vidtmkvmk wrote:
             | If an iguana loses its tail, it doesn't just bleed until
             | the new tail starts forming, right? Doesn't it seal up _and
             | then_ regrow?
        
           | tim333 wrote:
           | The only animal that really does it properly is the
           | salamander and I guess our family trees diverged before they
           | figured that out?
           | 
           | Still scientists are working on it - they've done frogs
           | https://www.nbcnews.com/science/science-news/can-humans-
           | regr...
        
             | 8n4vidtmkvmk wrote:
             | Can't just be the salamander. I saw an iguana regrowing its
             | tale just last week. Geckos too. Pretty sure starfish as
             | well.
        
       | croisillon wrote:
       | reminds me of the New Avengers episode "the eagle's nest"
       | https://www.imdb.com/title/tt0659325/
        
       | redundantly wrote:
       | > ...the wood frog's liver produces large amounts of glucose that
       | flushes into every cell in its body. This syrupy sugar solution
       | prevents the cells from freezing...
       | 
       | All natural, no preservatives added, sweetened frog popsicles!
       | Yummy!
        
         | crazygringo wrote:
         | I'm not even joking about this -- since frog legs are a thing
         | people eat (taste kinda like chicken), I'm now incredibly
         | curious what this would taste like if you "caught" these in
         | their frozen state and cooked them.
         | 
         | Are we taking syrupy sweet meat? Or just a hint of it?
        
           | lIl-IIIl wrote:
           | According to the video in https://shakerlakes.org/frozen-
           | frogs, it's also full of urine. Not sure if any left after
           | the cooking process.
        
       | olddustytrail wrote:
       | Ok, this is what you really wanted:
       | https://www.bbc.co.uk/programmes/p091w1b3
        
         | sandgiant wrote:
         | The content is not available in your location.
        
           | olddustytrail wrote:
           | Ah, sorry. I'll see if I can find an alternative source.
           | 
           | Edit: I can't and I'm busy. Any UK folk who can provide this
           | for our international family?
        
       | imglorp wrote:
       | Aquatic turtles have another brumination (hibernation) strategy.
       | Since they breathe air, yet may get trapped under the ice for
       | months at at time, they lower their metabolism plus have
       | adaptations such as scavenging some oxygen from the water via
       | rectal tissues as well as other chemical activity involving
       | glucose and calcium.
       | 
       | https://www.pbs.org/newshour/science/the-secret-to-turtle-hi...
       | 
       | https://wildlifeinwinter.com/painted-turtle
       | 
       | I have one at home in constant warm water, yet she can see the
       | sky and decides to bruminate on her own: every year around this
       | time, she starts napping in her under water hide for days or
       | weeks at a time.
        
         | card_zero wrote:
         | That's a word I don't know (and neither do you): brumation,
         | invented in 1965.
         | 
         | https://en.wiktionary.org/wiki/brumation
         | 
         | The verb ought to be _brumate._ But I guess the desire to pull
         | it into line with _hibernation_ is strong, this happens with
         | lots of words. (Or was the influence from  "rumination"?)
        
           | imglorp wrote:
           | Oops, thank you.
        
           | esperent wrote:
           | Weirdly, I do know this word and I learned it exactly two
           | days ago because I wanted to know the technical difference
           | between Hibernation and Sleep in Windows 11.
           | 
           | So I typed in "hibern..." to Kagi and it suggested
           | "hibernation vs brumation". I got sniped because I'd never
           | seen the word before. So yep, I did know. And probably would
           | have forgotten by next week if I didn't see your comment! But
           | now I'll remember forever, thank you.
        
         | Loughla wrote:
         | Our tortoise, even though he's in a very comfortable climate
         | controlled indoor pen, tries to dig a hole every year to do the
         | same.
         | 
         | He's not very smart but he tries hard.
        
       | zardo wrote:
       | How are they thawing from the inside out?
        
         | ledauphin wrote:
         | this seems implausible to me based on how heat generally
         | transfers...
        
       | pcthrowaway wrote:
       | Now I'm wondering all kinds of things about their brain. Are they
       | capable of forming memories, and would they retain those memories
       | after a freeze/thaw cycle?
       | 
       | Effectively they're dead when they freeze. I'm assuming there's
       | no brain activity.. Which means when they thaw they're being
       | restored to life. I wonder if any other animals experience this
        
         | card_zero wrote:
         | Reading about them, it seems they migrate in winter (like half
         | a mile uphill), but the adults always return to the same
         | breeding pond in spring, so that information is stored
         | somewhere.
        
           | jon_richards wrote:
           | I'm not surprised. There have been studies that show
           | caterpillars retain memories through metamorphosis into a
           | butterfly (a process that basically liquefies them).
        
           | yyyfb wrote:
           | Couldn't it just be algorithmic? Go uphill in the summer,
           | then downhill until you hit water, you'll probably end in the
           | same pond?
        
         | userbinator wrote:
         | Hibernate and resume.
        
         | bobbylarrybobby wrote:
         | I would assume that just as a computer's storage remains intact
         | after a restart, these frogs' brains have internal structure,
         | synaptic connections etc that are preserved after a freeze/thaw
         | cycle.
        
           | kortilla wrote:
           | That's a bad assumption though. Based on our inability to
           | bring brains back to life it seems very likely the better
           | comparison is volatile memory like RAM.
        
             | MacsHeadroom wrote:
             | As discussed elsewhere in this thread, butterflies retain
             | some memories formed as caterpillars after every cell in
             | their body changes drastically. They never die but they
             | change immensely and still retain memories, so I'm not sure
             | we know enough to say what good and bad assumptions are
             | here.
             | 
             | Also this should be relatively trivial to test.
        
               | gus_massa wrote:
               | The nervous system of caterpillars is not disolved during
               | the transformation.
        
       | mathh wrote:
       | Could such a mechanism be used for interstellar travel ?
        
         | vitiral wrote:
         | Of the frogs? Sure!
        
         | 8n4vidtmkvmk wrote:
         | No. Article says they have 1000x sugar in their blood. When we
         | get to 10x we tend to die.
        
           | ImHereToVote wrote:
           | We could replace some of the Glucose with Xylitol. Xylitol is
           | another sugar that protects the body from Advanced Glycation
           | End Products. AGEs are what makes glucose so dangerous.
           | 
           | https://www.healthline.com/nutrition/advanced-glycation-
           | end-...
        
         | RedNifre wrote:
         | Be careful though, if you send an all female human crew
         | enhanced by frog genes, some of them might change sex on the
         | way.
        
       | tantalor wrote:
       | > wood frogs spend the winter frozen
       | 
       | > syrupy sugar solution prevents the cells from freezing
       | 
       | So they don't freeze. This is click bait.
        
         | GavinMcG wrote:
         | Why does it only count if the cells themselves freeze? Also, am
         | I still allowed to play freeze tag, or do we need to rename
         | that?
        
         | vitiral wrote:
         | Can't tell if joking...
        
       | durkie wrote:
       | A great book about how animals survive super cold winters is
       | "Winter World" by Bernd Heinrich.
       | 
       | Bernd is a super fascinating biologist who really dives deep in
       | to things. At one point in the book I think he was talking about
       | chipmunks surviving winter, and it goes really fun on the first
       | principles. Something like: "chipmunks have a surface area of X
       | m^2, and need to maintain an internal temperature of Y@C. If the
       | outside temperature is -40@C they therefore they need to consume
       | Z calories per hour just to maintain body temperature. Their
       | favorite food are pine nuts from the white pine tree. The pine
       | nuts each have B calories, so the chipmunk will need to eat D of
       | them per hour. How many nuts can a chipmunk fit in its mouth?
       | Well I found a dead one and shoved pine nuts in to its mouth
       | until I couldn't fit anymore, and managed to get 17 in there.
       | That means..."
        
         | lukan wrote:
         | "Well I found a dead one and shoved pine nuts in to its mouth
         | until I couldn't fit anymore, and managed to get 17 in there."
         | 
         | That part is .. weird. What is the point except morbid humor?
         | 
         | Chipmunks have a food storage, so they don't store nuts in
         | their mouth. They wake up, go to the food storage and eat and
         | go back to sleep. They don't go outside to collect more nuts in
         | -40degC. Maybe that was the point of that calculation, to show
         | it would not be a good strategy, but there ain't much nuts in
         | winter on trees left anyway.
         | 
         | (and the calculation above lacks insulation of the fur and
         | their sleeping place)
        
           | speerer wrote:
           | > What is the point except morbid humor?
           | 
           | It'll be part of the time-spent-eating calculation, I expect.
           | 
           | Edit: I think I found the direct quote among a trio from a
           | Goodreads review, which gives more context [0]:
           | 
           | > To get a rough idea of whether the flying squirrel's nest
           | indeed affords much insulation, I heated a potato to simulate
           | the body of a squirrel and examined its cooling rates.
           | 
           | > I do not know how many seeds a chipmunk usually packs into
           | each of its two pouches--I easily inserted sixty black
           | sunflower seeds through the mouth into just one pouch of a
           | roadkill.
           | 
           | > Some years ago, I took on the brave, or foolish, task of
           | measuring hornets' body temperatures, grabbing and stabbing
           | them with an electronic thermometer as they left their nests.
           | 
           | [0] https://www.goodreads.com/book/show/19954417
        
           | jounker wrote:
           | The number of seeds that fit into a cheek pouch determines
           | calories transferred per trip to storage.
           | 
           | Knowing this number will be critical for any modelling of
           | chipmunk energetics.
        
             | lukan wrote:
             | They can also eat at the storage. Also some chipmunks have
             | their food storage next to their bed.
             | 
             | So knowing how big the cheeks are, is a useful information,
             | but in this context not clear.
        
       | abound wrote:
       | > Nobody yet understands what starts the wood frog's heart after
       | being frozen and inert for the entire northern winter.
       | 
       | To me, that's the most fascinating part of the (already quite
       | fascinating) story. Frog is frozen solid, there is no (to our
       | knowledge) heartbeat or brain activity. It thaws and _something
       | happens_ that gets it going again.
       | 
       | I have trouble imagining what that mechanism could even look
       | like. Tiny portion of brain responsible for keeping track of
       | frozen-ness? Some chemical signaling from within the body cavity?
        
         | chii wrote:
         | a chemical reaction that is sensitive to temperature.
        
         | telgareith wrote:
         | my poor knowledge of biology says it's a deeply unsatisfying
         | "heart beats when enough of the body has thawed for it to
         | beat."
         | 
         | If the heart depends on the brain at all in frogs.
        
           | nine_k wrote:
           | It doesn't, even in humans. There is an independent circuit
           | of special muscle cells right on it that keeps it beating.
           | 
           | So starting and stopping the heart in a controlled manner is
           | pretty interesting, because it has to be well-timed, and
           | there are few obvious and reliable inputs to control it,
           | especially when thawing.
        
             | 8n4vidtmkvmk wrote:
             | Why doesn't my heart keep beating if my brain dies then? Or
             | would it?
        
               | kampsun wrote:
               | I understand that it would keep beating until you are
               | provided oxygen. That's my understanding understanding of
               | brain death at least.
        
               | oharapj wrote:
               | Probably because the brain controls breathing and so the
               | oxygen runs out quickly
        
               | 8n4vidtmkvmk wrote:
               | I was going to say that's silly, but I guess we need to
               | be able to hold our breath sometimes.
        
             | telgareith wrote:
             | "Even in humans"- thats a pretty steep assumption.
             | 
             | I am fully aware that if the human heart is severed from
             | the brainstem it will develop _A_ rhythm.
             | 
             | But what about other mammals? Dunno. Not going to assume.
             | 
             | Theres also the tidbit that frogs are not mammals.
        
               | nine_k wrote:
               | The point is that whatever control a frog heart may have,
               | it's not cerebral. It must be a very low-level circuit,
               | which can be modulated from outside, but which remains
               | autonomous.
        
         | LoveMortuus wrote:
         | Maybe something like a bit flip but for neurons happens in the
         | frog's brain from the sun's radiation or something.
         | 
         | Or the elasticity of the heart and muscles.
        
           | necovek wrote:
           | If it gets frozen in a heavily contracted form, unthawing it
           | will trigger at least one half-beat: could that be enough to
           | restart it?
        
             | gnatolf wrote:
             | Hard to imagine that this is significant, given that the
             | (un)thawing is likely quite slow.
             | 
             | Did not think that my early morning would be spend trying
             | to imagine how thawing a frog works. :)
        
               | necovek wrote:
               | Good point for sure.
        
         | PittleyDunkin wrote:
         | The frog is _not_ frozen solid.
        
           | PhasmaFelis wrote:
           | Interestingly, you can freeze a rat solid in liquid nitrogen
           | --completely solid right through--and then thaw them out in a
           | microwave and they actually survive. Well, many of them
           | survive. For a while. Okay, it's not good for the rats but
           | it's still crazy that it works.
        
             | plastic3169 wrote:
             | Where can I read more about this? If this was a fact I
             | think people would be way more excited about cryonics.
             | Casually browsing wikipedia suggests that we are not there
             | yet with the ability to thawn large animals or even organs.
        
               | terribleperson wrote:
               | I can't source this because I read it years ago, but I
               | _believe_ that there is some science that says that a
               | major factor (not the only major factor, but a major
               | factor) is body mass and volume. Essentially, the speed
               | at which the core organs go from operating temperature to
               | frozen is crucial, and it 's also important for them to
               | be deprived of oxygen when that happens. Humans are
               | just... too big.
        
               | sriacha wrote:
               | There are ways to adjust cooling rates... for example
               | route the blood externally and chill it like they do in
               | some kinds of surgery.
        
               | blue_pants wrote:
               | From https://en.wikipedia.org/wiki/James_Lovelock
               | 
               | "In the mid-1950s, Lovelock experimented with the
               | cryopreservation of rodents, determining that hamsters
               | could be frozen and revived successfully.[14] Hamsters
               | were frozen with 60% of the water in the brain
               | crystallised into ice with no adverse effects recorded.
               | Other organs were shown to be susceptible to damage.[15]"
               | 
               | And there's a Tom Scott's interview with James Lovelock:
               | 
               | https://www.youtube.com/watch?v=2tdiKTSdE9Y
        
               | mock-possum wrote:
               | Wouldn't it be crazy if _only_ worked on hamsters
        
               | tim333 wrote:
               | I was checking youtube to see if I could see that happen
               | but failed - they were all about thawing rats to feed to
               | snakes. There was however a goldfish dropped in liquid
               | nitrogen briefly and recovering
               | https://www.youtube.com/watch?v=wwolYFGM9pU
        
         | m463 wrote:
         | What puzzles me is... it is not just the heart but the entire
         | circulatory system.
         | 
         | Maybe it is that thawing happens in reverse with the
         | extremities, then the rest of the system thawing, with the
         | heart being last. Would be a biological advantage in this case
         | for the heart to be centrally located.
        
           | Someone wrote:
           | > Maybe it is that thawing happens in reverse with the
           | extremities, then the rest of the system thawing, with the
           | heart being last
           | 
           | FTA: In spring, the wood frog thaws from the inside outward.
           | First the heart starts beating. Then the brain activates.
           | Finally, the legs move.
        
             | cmrx64 wrote:
             | The article says that, but it can't be the normal meaning
             | of thaw. Thermodynamically, onbiously the innermost portion
             | of the volume is going to warm last. But in terms of the
             | frog's system restart order, that order makes sense.
        
               | ethbr1 wrote:
               | The inside of the frog isn't actually frozen, because
               | it's glucose-flushed.
               | 
               | So the sequence is more:                  1. Outside of
               | frog thaws        2. ??        3. Heart starts beating
        
               | Someone wrote:
               | This frog is alive. It could detect that long-term thaw
               | is imminent (say from sensors on its skin) and start some
               | processes that produce heat around its heart.
        
         | abainbridge wrote:
         | I skimmed
         | https://en.wikipedia.org/wiki/Sinoatrial_node#Function. Here's
         | my guess at what is going on:
         | 
         | In humans (and I guess many animals), the thing that controls
         | the heart beat is a structure in the heart called the
         | Sinoatrial node. Each cell in the SA node has an ability to
         | generate its own rhythmic electrical impulse. I imagine that
         | when one of these cells thaws out in a Wood frog, it
         | immediately starts producing its rhythmic pulse. It has to get
         | in sync with the rest of the cells in the Sinoatrial node
         | before the heart will beat correctly, so the cells have a
         | mechanism to communicate their rhythm with their neighbours. I
         | guess each cycle, each cell adjusts its phase a little towards
         | the average phase of its neighbours and thus a consensus will
         | be reached.
        
           | mock-possum wrote:
           | Fireflies eventually manage to more or less sync up and
           | they're completely separate organisms - tiny cells with
           | physical connections inside the body should be able to make
           | it work.
        
         | agumonkey wrote:
         | Maybe the system relies on average heat randomness to bootstrap
         | again ..
        
         | xg15 wrote:
         | Yeah, I was wondering about that too, but on the other hand,
         | there are possibilities:
         | 
         | You don't need a brain to execute "programmed behavior" in a
         | body, the cells have enough "compute" in the regulatory
         | networks of their DNA, RNA and proteins to do that on their own
         | (and in fact do it all the time as part if their normal
         | functioning. That's what "metabolism" means.)
         | 
         | Another question would be where the cells take the energy to
         | execute that program if blood circulation has halted and there
         | is no oxygen. But then on the other hand, at that point they
         | are filled to the brim with glucose. So I wonder if this isn't
         | just to prevent freezing but also as an energy reserve for the
         | "restart".
         | 
         | (Sorry for the bad programming analogies in this post, please
         | don't take them too literally)
        
         | sandworm101 wrote:
         | Most likely it isnt that the heart stops or starts in response
         | to freezing. The heartbeat signal probably runs 24/7 unless
         | inhibited by freezing. So as soon as it thaws, the signaling
         | bit resumes its pulsing and the rest of the heart begins to
         | beats as it also thaws. The order in which organs thaw would be
         | regulated by sugar levels. Remember too that at this scale
         | frogs can absorb much of their needed O2 through their skin. A
         | steady heartbeat isn't as essential as it is with us.
        
       | LoveMortuus wrote:
       | I hope the frogs don't feel pain... Imagine getting frozen alive
       | and extremely slowly.
        
         | ssr2020 wrote:
         | The One who created it with care and compassion has arranged
         | its pain. Perhaps it is like us waking up from sleep."
        
       | m463 wrote:
       | looking at wikipedia, there is more information:
       | 
       | > Frogs can survive many freeze/thaw events during winter if no
       | more than about 65% of the total body water freezes
       | 
       | https://en.wikipedia.org/wiki/Wood_frog#Cold_tolerance
        
       | javajosh wrote:
       | _> Also, the wood frog's ability to withstand freezing may help
       | researchers discover how human organs used for transplants could
       | be frozen and thawed without damage. This would increase the
       | allowable time between removing an organ from a donor and
       | implanting it within the recipient, which could make many more
       | transplants possible._
       | 
       | While certainly some good would come of this, imagine the
       | unintented consequences of such an advancement, especially in a
       | world with stark income inequality.
        
       | w10-1 wrote:
       | For those thinking freezing could be an option... there's a fair
       | bit more than just avoiding ice crystals.
       | 
       | Medical hypothermia in humans (which is just a few degrees
       | colder) is bounded not really by not by time-cold but by
       | restoring normal temperature too quickly. It turns out
       | mitochondria generate a lot of free oxygen radicals when going
       | hypoxic, and restoring oxygen quickly does the chemical damage
       | that actually causes death - so they restore normal temps over a
       | period of hours. I'd bet that's not the only metabolic cycle
       | deranged by hypercooling.
        
         | ImHereToVote wrote:
         | Couldn't you inject liposomal ATP into the bloodstream before
         | the cooling process? Then mitochondria could simply be turned
         | off. (I don't mean simple when I use the word simply)
        
       | rendall wrote:
       | > _Understanding how frogs can do this might provide valuable
       | knowledge to help in the management of high blood sugar in people
       | with diabetes._
       | 
       | Also, space travel!
        
       | tim333 wrote:
       | One min video with some frozen frogs https://youtu.be/SSvspDZOVV0
       | 
       | They only seem part frozen - still a bit bendy when touched.
        
       | wkjagt wrote:
       | > No heartbeat. No breathing. For the entire winter, the wood
       | frog is like a lump of hard, frigid, icy stone carved in the
       | shape of a frog. But it's alive, in a state of suspended
       | animation.
       | 
       | I find it hard to grasp what is still alive about the frog. I
       | mean, it's not dead so there must be something happening inside
       | the frozen frog? How does it compare to a dead (and frozen) wood
       | frog?
        
         | fhfjfk wrote:
         | A live frozen frog has ice around it's cells. Inside the cells
         | remains liquid because of extra glucose acting as anti-freeze.
         | 
         | A dead frozen frog will be frozen completely, with all the
         | cells having ruptured walls from their insides freezing.
        
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