[HN Gopher] Making the Micropipette
       ___________________________________________________________________
        
       Making the Micropipette
        
       Author : bschne
       Score  : 132 points
       Date   : 2024-01-03 10:32 UTC (12 hours ago)
        
 (HTM) web link (press.asimov.com)
 (TXT) w3m dump (press.asimov.com)
        
       | bschne wrote:
       | For a semi-related case study of entrepreneurial tool-building in
       | science, I also liked this:
       | http://milan.cvitkovic.net/writing/neuropixels/
        
       | IggleSniggle wrote:
       | > What is something you do, day after day, that is arduous or
       | bothersome? Do you have a subtle itch or a pressing need to make
       | it easier?
       | 
       | If the answer is yes, consider spending a couple of days making
       | improvements to the process, as a frustrated Schnitger did in
       | 1957. Simple solutions to widespread problems can alter the
       | course of scientific history.
       | 
       | --
       | 
       |  _sigh_ I guess it 's the season for mucking around with my shell
       | again
        
         | gpderetta wrote:
         | Now I continue hacking at my .emacs without guilt and just need
         | to wait for the day I'll alter the course of history.
        
           | throwup238 wrote:
           | Is your Emacs Evil though? We might not want its course
           | altered.
        
         | fuzzfactor wrote:
         | Tis always the season.
         | 
         | >> What is something you do, day after day, that is arduous or
         | bothersome? Do you have a subtle itch or a pressing need to
         | make it easier?
         | 
         | This is what I faced for well over 45 years of deep exposure to
         | lab bench work from many different positions from student to
         | grunt to leadership.
         | 
         | Chemical commodities, not biochemical research though.
         | 
         | However as expected there is a certain amount of similarity in
         | some of the laboratory tasks regardless of the diversity of
         | scientific objectives.
         | 
         | I'm still willing to grab dangerous chemicals & apparatus by
         | the throat with my own bare hands if necessary for valuable
         | clients. Just not wanting to use my own throat, so I never
         | wanted to pipet by mouth either.
         | 
         | The vintage photo of Dr. Verder looks like she is filling a
         | measuring pipet, before capping the glass tube with a fingertip
         | then precisely dispensing manually as many scientists at the
         | time would do.[0]
         | 
         | Now it's fire-retardant lab coats and respirator-ready facial
         | hair as table stakes.
         | 
         | In my lab I had a vintage picture of Edison holding up a
         | chemical solution above eye level, observing it carefully
         | without safety glasses or PPE of any kind. His lab in the
         | background was "a little bit" cluttered with chemicals and
         | stuff. I didn't draw attenton to this kind of artwork, it was
         | hanging off to the side in the front office, I figured it spoke
         | for itself.
         | 
         | Plus I had more types of PPE than most are familiar with, which
         | I used carefully as needed. But that's not what I'm focusing on
         | right now.
         | 
         | Mouth pipetting was still common when I was getting started 30
         | years later than 1938, as a student so this is what had been
         | taught for many decades but you were supposed to be a realistic
         | judge about what risk there could be depending on what you were
         | pipetting. The rubber bulbs which can substitute for mouth
         | pipetting can be clumsy and not as precise and each person was
         | expected to draw the line at what laboratory solutions they
         | were going to avoid inhaling. These were often referred to as
         | Pasteur pipets and were still all-glass everywhere, even thin
         | disposable ones mostly until the 1980's when plastic
         | disposables took over.
         | 
         | But by the 70's there was growing interest in safety and
         | "rules" against mouth-pipetting were getting more widespread,
         | but it just made people look the other way when desperate
         | workers did it a little bit anyway, there was not usually an
         | effective formal replacement.
         | 
         | Ripe for disruption by mechanical means I would guess.
         | 
         | Maybe with increasing complexity to the mechanism I would say
         | it's mainly worthwhile for quite repetitive work.
         | 
         | Regular glass pipets are still everywhere and I was just lucky
         | to become a professional not long before a simple precision
         | pipet bulb was invented having little plugged vents in the
         | rubber that are controlled by careful squeezing.[1] People from
         | the chemical plants and refineries had never seen this, it was
         | only available from one company until 20 years later when the
         | patent expired. It was good for major company chemists and
         | engineers to be able to see me manually doing NBS-traceable
         | (now NIST-traceable) work with obvious clarity when stakes were
         | highest. They knew their own laboratories, which supported the
         | process units that actually made the chemicals, were way more
         | expensively built but still somewhat embarrassing sometimes
         | when it came to absolute certainty. Now decades later I'm not
         | alone, "everyone" claims to be NIST-traceable, I still say not
         | so fast. When I came to my employer almost a decade ago I had
         | to kick out the mechanical pipets and replace them with
         | serialized traceable manual traditional glass type, with major
         | retraining for this exact reason. [2] Expert witnessing is more
         | critical than routine work.
         | 
         | I always liked the Gilson salesman, and Ranin too but I never
         | bought anything since the stakes are so high with the big
         | ships, and your ship doesn't come in every day anyway so
         | there's not that kind of repitition. But a couple barges aren't
         | too bad either. So I've got a lifetime of ideas that other
         | people could use where I didn't go far enough to actually even
         | flush out a prototype since I didn't need it for myself at the
         | time. Over the decades I've watched some of the things I had
         | come up with, be recognized and commercialized by capable
         | operators much later. Some of the most obvious things are the
         | most commercializable and it was going to happen anyway. Every
         | milestone or breakthrough doesn't have to be that elusive to be
         | good. It doesn't bother me to be late to market when I'm not in
         | the market to begin with. It's a good feeling knowing there's
         | more where that came from all the time, and early ideas that
         | nobody else has touched have now stood the test of time without
         | competition developing. Plus if I had patented everything most
         | of it would be expired by now.
         | 
         | A lot of the chemical plants only make a handful of different
         | chemicals at each location, and usually only a few of those are
         | actually commodities. That limited number of chemicals are the
         | ones that each of their labs is designed and built to handle
         | 24/7, and some tasks can get pretty repetitive for them. But
         | when you regularly work with material from all over the place
         | you really get ideas for inventions for all kinds of different
         | things. Especially with emerging instruments and techniques
         | when there aren't so many worthwhile publications to go on. So
         | it can be a slippery slope and you end up inventing something
         | every day. Never comercialized very much yet, too busy, a lot
         | is not worth money anyway or would require becoming a
         | capitalist of some kind where the terms are not very
         | attractive. I did OK getting paid for what I could do rather
         | than how much I sold. Well I enjoyed sales starting as a
         | teenager and eventually starting my own commercial lab,
         | scientific instrument sales was supposed to be a future effort
         | but I did not approach that milestone close enough with any
         | prototype before natural disaster set it back where you can not
         | make up for lost time. I also had a framed vintage picture of
         | Hewlett & Packard posing with their original invention, hanging
         | back there.
         | 
         | The edge of the bench can be like a goal line where you can
         | make field goals from a reasonable distance (if your desk or
         | office is not too far from the bench) but you can only actually
         | score a touchdown by crossing the line yourself. Extra points
         | count for twice as much too when somebody personally carries
         | the ball across the line rather than kicking it in "remotely".
         | Plus in old labs there has never been enough space on the bench
         | for PC's because they weren't built for it, they were just
         | crammed in. I know. I did it myself.
         | 
         | I still like to build labs from the ground up, it's a hoot.
         | 
         | [0] In the backgroud of the photo there's a vat on a low wooden
         | bench, that is a precision heating bath where the electronic
         | temperature control consists of the logic necessary to turn off
         | the heating element when the mercury in a special wired
         | thermometer rises to make contact with a thin wire reaching
         | into the inside of the glass from the top. The depth of the
         | wire was adjustable with a knob at the top of the thermometer,
         | not unlike the knob that would later appear at the top of the
         | adjustable mechanical pipets to easily adjust their volumes. I
         | was one of the last to use a bath of this vintage which I had
         | gotten surplus and never thought I would ever restore. This
         | does remind me that years later, but still before digital, when
         | we needed a 0.001 degree tolerance for another location I
         | rapidly designed an analog temperature controller using a
         | solid-state sensor at the bottom of a glass tube, with the
         | circuitry at the top in a small enclosure to run on an external
         | bipolar power supply. I needed it to be a drop-in replacement
         | for the glass mercury ones that were still common in the much
         | more modern baths that were still in use. Plus it had to be
         | built with as many parts as possible from Radio Shack, ended up
         | the only thing I needed from elsewhere was a gigohm resistor.
         | Another one of those everyday inventions. Once digital appeared
         | it took years before this precision could be reached.
         | 
         | [1] https://www.coleparmer.com/i/silicone-pipette-
         | filler-3-way-v... Now these are available in many polymers
         | other than natural rubber, with openings plugged with different
         | materials than the original stainless steel ball bearings.
         | 
         | [2] Also kicked out IT since they weren't very good with
         | chemicals (or even electronics) but that's a different story
         | altogether.
        
       | zabzonk wrote:
       | speaking as an ex microbiology/immunology technician, these
       | things are brilliant - i really can't imagine doing the job
       | without them.
        
       | bentobean wrote:
       | > "... it is oddly fitting that a man best known for creating a
       | device to move around small volumes of liquid died unable to
       | manage large ones."
       | 
       | Ouch. This felt... a bit unnecessary and out of place.
        
         | bschne wrote:
         | yeah they missed the tone a bit there, tripped over it too
        
         | op00to wrote:
         | I came here to post that. Totally uncalled for and not funny in
         | the least. It is also not fitting at all.
        
           | stronglikedan wrote:
           | It was funny and an appropriate levity break. I laughed out
           | loud. I hope they leave it in, despite the author's request
           | to have it removed. The editors knew best in this case.
        
             | op00to wrote:
             | Can you explain what you found funny? Everyone is entitled
             | to their own sense of humor, but I don't see how a drowning
             | death is funny because it happened to a scientist that
             | invented a tool.
        
               | riversflow wrote:
               | Not OP, but I'll take a stab.
               | 
               | > funny because it happened to a scientist that invented
               | a tool.
               | 
               | ..for transferring fluids.
               | 
               | I don't think much of Freudian psychology as a whole, but
               | he's got a relevant quote that is pretty good on Gallows
               | humor, from _Der Humor_ 1927:
               | 
               | > "The ego refuses to be distressed by the provocations
               | of reality, to let itself be compelled to suffer. It
               | insists that it cannot be affected by the traumas of the
               | external world; it shows, in fact, that such traumas are
               | no more than occasions for it to gain pleasure."
               | 
               | The comedy, in this light, is a result of the dissonance
               | that someone smart and savvy enough to invent an
               | extremely useful tool for moving liquids died in a large
               | body of liquids not long after said invention.
               | 
               | My guess is that people who find the humor here relate
               | more to the drowning scientist directly. Dying from
               | drowning in an Alpine lake, or just drowning, isn't that
               | common of a risk, but if it is a risk you are familiar
               | with you're more likely to find the humor.
               | 
               | At least thats the case for me.
        
               | op00to wrote:
               | Just so I follow, if the inventor of the ez bake oven
               | died in a home gas explosion, that's sort of the same
               | concept at play?
               | 
               | Thank you for the explanation.
        
         | Metacelsus wrote:
         | You're right, this was rather tone-deaf. It wasn't in my
         | original draft and was added by an Asimov editor in an attempt
         | to make the piece more interesting. Although I did review the
         | final article proof, so it's my responsibility too. I've made a
         | request for Asimov to edit it.
        
           | BiteCode_dev wrote:
           | For the record, I find it achieves the editor's goal for me.
           | 
           | A bit of dark humor can be a nice touch.
           | 
           | The problem is rather if you are ok with the fact a part of
           | your audience will be offended with it.
        
             | Metacelsus wrote:
             | You know, this might be a classic case of "Your Incentives
             | Are Not The Same As Media Companies'"
             | 
             | https://www.astralcodexten.com/p/your-incentives-are-not-
             | the...
        
             | jsight wrote:
             | It seems like slightly different wording would have fixed
             | it. "Oddly fitting"? We don't usually describe untimely
             | deaths as "fitting". Perhaps "ironic" or something along
             | those lines would fit the tone better.
        
               | op00to wrote:
               | If the pipette dude would have died of drowning by
               | pipetting, that would be ironic. If he drowned from a
               | microlitre of water, that'd be ironic. But saying he
               | couldn't handle large amounts of water is weird. The
               | dude's unfortunate drowning death really doesn't add much
               | to the story.
        
       | bobmaxup wrote:
       | I thought these devices were called pipettors not strictly
       | micropipettes?
        
         | kortex wrote:
         | A pipettor is an agent which does the pipetting, usually this
         | is the scientist when manually pipetting, but there are
         | autopipettors which are robots.
         | 
         | Pipettes are originally the glass tubes of fixed or graduated
         | volume, but the definition has expanded to include air
         | displacement pipettes aka "micropipettes" (even when the volume
         | is measured in milliliters not microliters, they are still
         | colloquially micropipettes).
        
           | dekhn wrote:
           | Everything I've ever seen says that pipettor is in fact the
           | pipette with additional components, not the scientist or the
           | robot. https://www.sigmaaldrich.com/US/en/products/labware/li
           | quid-h...
        
             | fuzzfactor wrote:
             | It's just terminology, "regular" pipets dispense mainly in
             | milliliters, micropipets dispense in microliters.
             | 
             | The pipet is considered the tube, the pipettor is the
             | mechanical attachment, I would say that is whether the
             | attachment is with lips & tongue or not.
        
       | Metacelsus wrote:
       | Author here. I'm happy to answer any questions.
        
         | seanc wrote:
         | Thanks! My daughter works with pipettes all day long, and this
         | is fascinating. One of her favorite aphorisms is "Don't lick
         | the science!" and today I learned that apparently we used to
         | lick the science.
        
           | Metacelsus wrote:
           | Even today mouth pipettes are still used occasionally (in
           | embryology, for example).
        
             | dekhn wrote:
             | I use a mouth pipette (really just a small piece of glass
             | and some rubber tubing with a filter to transfer
             | tardigrades to my microscope slide. This was recommended to
             | me by a tardigrade expert. I had previously used bulb for
             | large glass pipette (my high school lab had a big sign "DO
             | NOT PIPETTE BY MOUTH") and micropipettes for decades, and I
             | was blown away with the level of control you get from mouth
             | pipetting. I'm not that bothered by inhaling some
             | tardigrades.
             | 
             | But being lazy one of my side projects is to make an
             | automatical tardigrade pipettor (ML-based with motors and
             | stuff).
        
         | sopchi wrote:
         | I am surprised there is on mention of rubber bulbs. If the main
         | concern is safety, as the beginning of the article seems to
         | imply, the rubber bulb would have been enough of a solution.
         | The micropipette solves mostly all the other concerns: speed,
         | repeatability, avoidance of contamination, ease of use one-
         | handed. A great invention for sure. Why would they even mouth
         | pipette if they can use a rubber bulb? Is it that the rubber
         | bulb was somehow invented later? Did you encounter anything
         | about that when researching this topic?
        
           | dekhn wrote:
           | it's all about control. You get a lot more feedback and
           | accuracy with your mouth.
        
             | sopchi wrote:
             | OK, I see. I still find that surprising because I would
             | imagine having the pipette hanging from your mouth doesn't
             | allow ou to really see what is going on if you are really
             | trying to hit a target volume. I would have thought the
             | feedback you need is visual.
        
               | function_seven wrote:
               | I just looked up photos of people pipetting by mouth.
               | They use tubes.
        
               | dekhn wrote:
               | Oh. I'm actually looking at the pipette tip under a
               | microscope (in my case it's clear glass that I pulled to
               | a fine tip using heat) and I can see the tardigrade get
               | sucked into the tip when I gently pull with my mouth. And
               | then I can see it whoosh back out when I push. The actual
               | volume doesn't matter, just that I pick a single
               | tardigrade and place it where it needs to go.
               | 
               | The other folks pipetting by mouth are actually looking
               | down at the tube and see the fluid volume reaches a
               | particular well-defined line on the glass pipette. If you
               | can taste the fluid, you've gone too far.
        
       | dmd wrote:
       | > A typical PhD student in the biosciences will use about 200,000
       | pipette tips over the course of their research. (An estimated ten
       | boxes of 96 tips per week, with 50 working weeks per year over a
       | 5-year period, comes out to 240,000 tips. And five years is a
       | generous estimate for completion of a PhD in the biosciences.)
       | 
       | This seems ridiculously high to me. If the student works 6 days a
       | week, 10 hours a day, they would be using a new tip about every
       | 3-4 minutes nonstop, every single hour of those 10 hours a day 6
       | days a week for 5 years. No reading, no writing, no meetings -
       | just pipette tips every 4 minutes, 10 hours a day for 5 years.
       | 
       | Is that really the life?
       | 
       | edit: Oh. Machines. Right.
        
         | bschne wrote:
         | was wondering the same thing!
        
         | BiteCode_dev wrote:
         | You assume they do it manually.
         | 
         | Today we have machines that can batch processes using pipettes,
         | and they can burn them at a fast rate.
        
         | gipp wrote:
         | Even when done by hand, you're also generally going to use a
         | large batch all at once to create a panel of different tests
         | under varying conditions. So it's more like you're using a new
         | tip about every 5-10 seconds for 15 minutes or so, twice a day.
        
           | margalabargala wrote:
           | Multi-pipettes are made, as well, here's a 64-channel one:
           | https://bioventures.com/products/capp%C2%AE-64-channel-
           | pipet...
           | 
           | When you're using 64 tips at a time, it's easy to imagine
           | using ten boxes of 96 tips per week. Ten boxes of tips will
           | fill that pipette only 15 times. So you're using your
           | multipipette twice a day.
        
         | Metacelsus wrote:
         | >Is that really the life?
         | 
         | Yes it is! For example, a simple 96-well plate qPCR experiment
         | can use 192 tips. It's easier with multichannel pipettes
         | though.
         | 
         | So it's more like: use 200 tips in the morning, then do reading
         | and meetings the rest of the day.
        
         | kortex wrote:
         | I've gone through a box a day at peak just doing regular
         | chemistry (biochem uses way more) so I completely believe it.
         | 
         | It's not like one pipet tip a minute. It's more like, you have
         | N source vials and M destination vials/wells/rows, and you'll
         | do that several times a day, interspersed with other processes.
         | A cycle goes like:
         | 
         | 10 New tip
         | 
         | 20 Suck up sample from source n
         | 
         | 30 Dispense into m-sub-i
         | 
         | 40 while you still have destinations for the current solution,
         | goto 20
         | 
         | 50 eject tip
         | 
         | 60 goto 10
         | 
         | This cycle takes maybe a few seconds to half a minute. Also
         | multiply number of tips by P if you have a multi-tip pipette.
        
         | flobosg wrote:
         | > a new tip about every 3-4 minutes nonstop
         | 
         | Pipetting and changing tips are a matter of seconds, not
         | minutes.
        
       | SAI_Peregrinus wrote:
       | > Interestingly, Suovaniemi didn't attribute his inventions to
       | diligence or tireless ambition, but quite the opposite. "I'm
       | extremely lazy by nature," Suovaniemi once told an interviewer,
       | so "I created a pipette with nine channels."
       | 
       | Engineering is all about laziness. The whole field is about
       | making devices & structures to reduce overall labor required.
       | Engineers need a certain sort of laziness, a willingness to work
       | now in order to work less later (or to help others work less
       | later). Tireless ambition rarely leads to good engineering, it
       | leads to continued drudgery.
        
         | adolph wrote:
         | I think 1319 better represents this comment than 1205.
         | 
         | In addition to laziness/efficiency, curiosity and lateral
         | thinking are helpful qualities.
         | 
         | https://xkcd.com/1319/
         | 
         | https://en.wikipedia.org/wiki/Lateral_thinking
        
           | robocat wrote:
           | What is the law of engineering that causes an abundance of
           | complexity?
           | 
           | There's some entropy law of abstraction that is the opposite
           | of Occam's razor: take a simple idea and embellish it until
           | it is sophistimicated enough to require total reengineering
           | to decomplexify it.
        
       | gertrunde wrote:
       | One feels compelled to mention the family member who learned the
       | hard way of the dangers of mouth pipetting... while working in a
       | bovine sperm collection facility....
        
         | ta988 wrote:
         | This just hurts one's ego, try that with acids/bases or highly
         | toxic compounds.
        
           | HeyLaughingBoy wrote:
           | One of the first medical instruments I developed code for
           | featured automated precision pipettes (precision measured in
           | single microliters) for sampling reagents and human body
           | fluids.
           | 
           | We had a guest once who used to be a lab nurse and mentioned
           | using mouth pipettes. The looks of horror on the scientists
           | in the room was palpable!
        
         | waltbosz wrote:
         | Interesting, I too heard of mouth pipetting via an antidote
         | about a modern day bovine sperm collection facility.
         | 
         | I was surprised that anyone still did it given the availability
         | of the micropipett.
         | 
         | Also, I wonder why the scientists of the past didn't obtain the
         | liquid samples by simply sticking their thumb over one end of a
         | straw dipped in the liquid.
        
           | adolph wrote:
           | > sticking their thumb over one end of a straw dipped in the
           | liquid
           | 
           | Brings back memories of learning how to do that with my
           | siblings. Every chance we got, we would scrunch up straw
           | wrappers and then use the method to animate the wrapper with
           | liquid.
           | 
           | Not certain if I should show my kids how to do that.
        
           | seanc wrote:
           | The pipettes I worked with in school 30 years ago were
           | effectively glass straws with a mark for the designated
           | volume. One dipped it in the solution over the mark, used the
           | thumb to seal it, and then carefully let tiny amounts of air
           | in to drop the bottom of the miniscus down to the line.
           | 
           | It was terrible and nothing ever worked and my thumbs hurt.
        
         | kragen wrote:
         | that sounds pretty safe; hiv and cmv don't infect cows. what
         | are the dangers?
        
         | analog31 wrote:
         | My mom pipetted by mouth in grad school in the 50s, doing
         | radiochemistry.
        
           | bell-cot wrote:
           | A bit further back -
           | https://en.wikipedia.org/wiki/Radium_dial
           | 
           | > Radium dials were typically painted by young women, who
           | used to 'point' their brushes by licking and shaping the
           | bristles prior to painting the fine lines and numbers on the
           | dials. This practice resulted in the ingestion of radium,
           | which caused serious jaw-bone degeneration and malignancy and
           | other dental diseases. The disease, radium-induced
           | osteonecrosis, was recognized as an occupational disease in
           | 1925 after a group of radium painters, known as the Radium
           | Girls, from the United States Radium Corporation sued. By
           | 1930, all dial painters stopped pointing their brushes by
           | mouth. Stopping this practice drastically reduced the amount
           | of radium ingested and therefore, the incidence of
           | malignancy.
        
       | pugworthy wrote:
       | There are some pretty nifty modern replacements for the pipette
       | for automated well plate work specifically. HP has one such
       | product, which I work on.
       | 
       | It is a "digital dispenser" that leverages inkjet technology to
       | dispense complex, multi-fluid protocols with picoliter to
       | microliter accuracy over about any type of well plate quite
       | quickly and effectively.
       | 
       | The basis of the product is a one-time-use cassette that
       | incorporates dispense heads and fluid reservoirs which the user
       | fills with their own fluids. Depending on the model, the cassette
       | can work with 1, 4, or 8 fluids at a time.
       | 
       | The latest new cassette type can sense and dispense single cells
       | into wells with a high level of occupancy and viability.
       | 
       | I know this comes off as a bit of a sales pitch, but it's a
       | product I'm genuinely excited to work on and our customers seem
       | quite pleased with it as well. If you search for "hp digital
       | dispenser" you'll find more info on it.
        
         | HeyLaughingBoy wrote:
         | picoliter accuracy is impressive!!!
         | 
         | What's your precision (dispense coefficient of variation) like?
         | When I was working with automated pipettes, we found that in
         | order get down to single-microliter precision we had to "touch
         | off" on the dispensing surface to use surface tension to pull
         | the last drop off consistently. However, our aspirate/dispense
         | ranges were pretty wide -- around 15uL to about 50mL from the
         | same device.
        
           | pugworthy wrote:
           | Not sure the exact numbers, but the fluid is jetted so there
           | is not really an issue with surface tension.
           | 
           | It's also no problem to do 1536 plates and even some nano-
           | well setups using custom well plate definitions.
        
         | varjag wrote:
         | HP as in Agilent or as in HP printer division?
        
           | pugworthy wrote:
           | HP printer. Based in Corvallis.
        
       | dekhn wrote:
       | Speaking of science tools, one of my favorite is the Worm Pick.
       | Researchers working with C.Elegans (one of those most common
       | model organisms) use worm picks to pick up and transfer worms
       | from one plate to another. The pick is made out of a metal that
       | can be heated up (to sterilize) many times. Such as platinum or
       | kanthal. Often, new researchers will craft their own worm pick
       | rather than purchase it. Originally, reseachers used eyelashes as
       | worm picks(!)
        
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