[HN Gopher] Base editing: Revolutionary therapy clears girl's in...
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       Base editing: Revolutionary therapy clears girl's incurable cancer
        
       Author : sonabinu
       Score  : 659 points
       Date   : 2022-12-11 02:35 UTC (20 hours ago)
        
 (HTM) web link (www.bbc.com)
 (TXT) w3m dump (www.bbc.com)
        
       | fastball wrote:
       | In response to the multiple people asking if base editing and
       | CRISPR are the same, the answer is: not quite. Same idea,
       | different enzyme.
       | 
       | The enzyme used by CRISPR is Cas9 (which is why you'll often see
       | it referred to as CRISPR-Cas9), which acts like a pair of
       | molecular scissors and cuts strands of DNA at a specific point in
       | the genome.
       | 
       | Base editing is a newer technique and utilizes an enzyme called
       | deaminase, which can change the chemical structure of a base pair
       | without doing the "cutting" part. There are specific deaminases
       | based you use depending on which base pair you're trying to
       | modify.
       | 
       | To oversimplify a bit: base editing is a more precise version of
       | CRISPR, which is less likely to cause more changes than actually
       | desired, as is a problem with CRISPR. That doesn't make CRISPR
       | obsolete though, because if your goals actually _is_ to remove
       | entire portions of a genome, CRISPR is a more effective
       | technique. Regardless, there is a lot of overlap between the
       | methods: both usually use adenoviruses as the delivery vehicle,
       | you can use both enzymes in tandem, etc.
        
         | nine_k wrote:
         | The word more understandable for the software developer crowd
         | would be "patching". That is, changing a base pair in place.
        
           | govg wrote:
           | I took it to mean doing an in place patch / removal of code
           | (CRISPR) vs doing a more general refactor that results in
           | cleaner code (base pair editing).
        
         | stanford_labrat wrote:
         | For clarification, most base editors are a fusion protein with
         | cas9 and the actual editing protein.
        
       | [deleted]
        
       | chrisweekly wrote:
       | IMHO this is a good reminder that the word "incurable" means "as-
       | yet unsolved", it's not an absolute.
        
       | stevespang wrote:
        
       | pkaye wrote:
       | Hasn't this treatment been use for a while now?
        
         | [deleted]
        
       | TaupeRanger wrote:
       | I'm not sure it counts as "revolutionary" if it has helped only 1
       | person, and maybe as many as 10-12 per year. It's really the
       | opposite of revolutionary, though I am very happy for this family
       | and their daughter.
        
         | ryangittins wrote:
         | Sure, there is one airplane but that's hardly revolutionary. A
         | couple of guys can fly a few dozen feet. So what?
        
           | selimthegrim wrote:
           | This is like if Larry David did an ad for the Wright brothers
        
             | HiroProtagonist wrote:
             | Pretty, pretty, pretty good.
        
         | TulliusCicero wrote:
         | This is like the ultimate form of Unimpressed Internet Guy.
         | 
         | The article even talks about other trials also underway, so the
         | parent comment doesn't even make any sense:
         | 
         | > Dr Robert Chiesa, from the bone-marrow transplant department
         | at Great Ormond Street Hospital, said: "It is extremely
         | exciting. Obviously, this is a new field in medicine and it's
         | fascinating that we can redirect the immune system to fight
         | cancer."
         | 
         | > The technology, though, only scratches the surface of what
         | base editing could achieve.
         | 
         | > Dr David Liu, one of the inventors of base editing at the
         | Broad Institute, told me it was "a bit surreal" that people
         | were being treated just six years after the technology was
         | invented.
         | 
         | > In Alyssa's therapy, each of the base edits involved breaking
         | a section of genetic code so it no longer worked. But there are
         | more nuanced applications where instead of switching an
         | instruction off you can fix a defective one. Sickle-cell
         | anaemia, for example, is caused by just one base change that
         | could be corrected.
         | 
         | > So there are already trials of base editing under way in
         | sickle-cell disease, as well as high cholesterol that runs in
         | families and the blood disorder beta-thalassemia.
        
           | [deleted]
        
         | Firmwarrior wrote:
         | Haha, your comment is like something a venture capitalist would
         | say when he's trying to lowball a startup founder in the show
         | "Silicon Valley"
        
         | quickthrower2 wrote:
         | There is a time when every revolutionary invention had only
         | helped one person, so far.
        
         | tinktank wrote:
         | It's a proof-of-concept. That's the hard bit done.
        
         | flobosg wrote:
         | > a revolutionary product, process, or idea is new and exciting
         | and not like anything that has existed or been done before
         | 
         | --https://dictionary.cambridge.org/dictionary/english/revoluti.
         | ..
        
         | 1123581321 wrote:
         | The technique is new and will be adapted to other cancers and
         | diseases.
        
           | walnutclosefarm wrote:
           | Actually, it's the other way around. The treatment described
           | is an adaption of CAR-T (Chimeric Antigen Receptor T-cell)
           | therapy, which was approved for use in the United States in
           | 2017, to the patient's specific form of leukemia. It's a
           | challenging adaptation because the cancer cells being
           | targeted are the same type of T-cells that are used in CAR-T
           | therapy, thus requiring the use of donor T-cells, and a
           | number of modifications that are unnecessary when CAR-T is
           | used for other cancers. Base editing makes that easier than
           | earlier forms of CRISPR. But base editing isn't the therapy
           | here, CAR-T is, and while CAR-T is certainly relatively, this
           | patient is not the first to receive, just the first with her
           | particular leukiemia.
        
             | SalmoShalazar wrote:
             | No, the parent comment is totally fine. The technique is
             | indeed new and yes will be applied in the treatment of
             | other diseases in the future. Don't know why you went to
             | open with a contrarian tone.
        
             | 1123581321 wrote:
             | I don't see the disagreement! Good info; thanks.
        
         | tsol wrote:
         | First it had to be accomplished, then they can work on getting
         | the price down
        
       | DoreenMichele wrote:
       | In layman's terms: She had a particularly vicious form of cancer
       | where the very cells that are supposed to protect your health --
       | your T-cells -- are the enemy. So they made three edits to donor
       | cells and wiped out her compromised cells. Then gave her a bone
       | marrow transplant afterwards to replenish her immune system with
       | new cells.
       | 
       | I'm squeamish about genetic research, but this looks really good
       | to me. I hope she recovers fully and there are no further
       | complications, though I imagine it's too early to guess what her
       | prognosis might be.
       | 
       |  _So there are already trials of base editing under way in
       | sickle-cell disease, as well as high cholesterol that runs in
       | families and the blood disorder beta-thalassemia._
       | 
       | That's a big deal for people with those genetic disorders.
        
         | mcculley wrote:
         | > I'm squeamish about genetic research
         | 
         | Can you elaborate on what makes you squeamish about research?
        
           | hotpotamus wrote:
           | Have you ever tried making changes to a large, incredibly
           | complex codebase with zero comments? Sometimes the changes
           | you make don't have the expected results.
        
             | dalmo3 wrote:
             | Also, stateful code, no staging environment, no undo
             | commit, prone to fatal runtime errors...
        
             | svnt wrote:
             | You've never made a change to such a codebase that was
             | generated by a massively parallel evolutionary computer
             | running for billions of years.
             | 
             | You could just as easily make the argument that there is
             | probably no body of data more stable than existing genomes.
        
             | mcculley wrote:
             | That sounds like an argument in favor of research.
        
           | yitianjian wrote:
           | Not OP, but something just feels unnatural and unsettling
           | about changing humans, and especially for changing yourself.
           | It feels like the uncanny valley where it just doesn't feel
           | right. I'm agnostic so it's not for a particularly religious
           | reasons, and it's purely an emotional response for me that's
           | not grounded in logic at all, so I also just describe it as
           | squeamish for no reason.
        
             | sinenomine wrote:
             | Thank you for a honest response. I think this is an
             | underexplored topic - how many of our broad social policies
             | were shaped mostly on the basis of sufficiently broadly
             | shared visceral feeling.
             | 
             | Should we base ultimately coercive society-wide regulatory
             | framework on mere "squeamishness", appropriately
             | rationalized, or should we instead embrace a non-
             | discriminatory, laissez-faire approach in this domain?
        
               | quesera wrote:
               | > how many of our broad social policies were shaped
               | mostly on the basis of sufficiently broadly shared
               | visceral feeling
               | 
               | All of them? I'm struggling to think of a counterexample.
        
               | vcxy wrote:
               | Yeah, I'd say the phrases "broadly shared visceral
               | feelings" and "broad social policies" point to nearly the
               | same concept.
        
             | BurningFrog wrote:
             | When I have these thoughts, I remind myself that typhoid is
             | natural and penicillin is artificial.
             | 
             | A big part of progress is fighting and defeating the
             | natural.
             | 
             | That said, not all genetic manipulation will end well.
        
           | DoreenMichele wrote:
           | Some people are interpreting that more broadly than I
           | intended it. I'm squeamish about this kind of medical gene
           | editing in specific.
           | 
           | There is a genetic disorder that runs in my family. I used to
           | be on a bunch of email lists for the condition. People with
           | the condition routinely volunteer for drug studies in a
           | desperate attempt to get help that current medical science
           | can't provide them and/or to get free care for a condition
           | that's very medically expensive.
           | 
           | People with serious medical conditions are all too often
           | treated like lab rats whose lives don't really matter.
           | Treating people like me humanely isn't a real high priority.
           | Quality of life is not much part of the discussion. Keeping
           | us alive a little longer is the only metric that seems to
           | count and horrifying things get done in the name of it.
        
           | javajosh wrote:
           | _> Can you elaborate on what makes you squeamish about
           | research?_
           | 
           | Yes, I for one can't imagine why editing the genes of humans
           | would cause alarm. After all, who wouldn't want to have
           | children that are smarter, more docile, and healthier? Or,
           | for other people's children, who have qualities that make you
           | uncomfortable and can't be edited out, like dark skin or
           | homosexuality, you have the option to edit their reproductive
           | ability so that such qualities can come to an end humanely,
           | without violence.
           | 
           | In the same way, I can't understand are those people against
           | longevity research. Who wants to die? Not me, and certainly
           | not those in power. You know, the people in power earned
           | their positions, they are good at it, and mortality just
           | means someone new and inexperienced will fumble around at the
           | job. For continuity and safety, immortal leaders will do a
           | better job and keep us safe. I don't see why anyone would
           | consider this a bad thing.
           | 
           | https://en.wikipedia.org/wiki/Satire
        
             | mcculley wrote:
             | I am, of course, aware of these tropes. If you have any
             | sincere objections to research, please enlighten us.
        
               | javajosh wrote:
               | You think that sincerity is incompatible with satire?
        
               | mcculley wrote:
               | Of course not. Satire can be done very well and
               | sincerely.
        
               | rfrey wrote:
               | I'm not sure how satire is relevant to this exchange.
        
               | last_responder wrote:
               | When it is mixed with the stupidity you spouted , yes.
        
           | biophysboy wrote:
           | Not the OP, but I think there are ethical concerns about
           | genetic manipulation that do not amount to catastrophizing
           | (e.g. 20th century eugenics is back!).
        
             | mcculley wrote:
             | Maybe the conflation is the problem for me. I don't see how
             | research or even consensual application necessarily leads
             | to "20th century eugenics".
        
               | SoftTalker wrote:
               | I don't see how it doesn't. Look at human history.
        
               | mcculley wrote:
               | I remain unconvinced that the inevitable existence of
               | e.g., designer babies will inevitably lead to forcing
               | people to have them.
        
               | dalmo3 wrote:
               | Consensual application is a huge assumption based on
               | recent history.
        
               | mcculley wrote:
               | The existence on non-consensual application would not
               | change my opinion on the value of research.
        
             | kristopolous wrote:
             | If we get designer babies along with curing cancer I say
             | "so be it".
             | 
             | Laws can be written to limit its use if we find it morally
             | objectionable
        
             | BurningFrog wrote:
             | The problem with old school eugenics was that it wasn't
             | consensual. Like forced sterilizations and abortions.
             | 
             | If gene tech can make future generations healthier and
             | smarter with informed choice by the parents, those future
             | generations will look back in horror at the barbaric pro
             | death & disease lobby of the 2020s.
        
         | emmelaich wrote:
         | Very interesting, because T-cells are implicated in multiple
         | sclerosis.
         | 
         | And probably other similar diseases.
        
           | lostlogin wrote:
           | The detective work going on with MS is fascinating. The blood
           | work from the US military and what it has shown is really
           | interesting - it's not new news but was to me when I stumbled
           | on it this year.
           | 
           | https://www.nih.gov/news-events/nih-research-
           | matters/study-s...
        
             | lebuffon wrote:
             | I have long suspected that viruses are the underlying cause
             | of most of the diseases that have eluded us for the last 50
             | years. HIV gave us insights into a novel way that viruses
             | can operate. It makes sense to think that there are more
             | ways than we imagined.
             | 
             | E/B virus is suspect in Type 1 Diabetes too. E/B
             | Vaccination could eradicate a group of diseases
             | potentially.
        
         | inglor_cz wrote:
         | Resetting the immune system can be already done and it seems to
         | help against auto-immune diseases such as lupus, but it is very
         | dangerous. Once you destroy the immune system of the patient,
         | and before it grows back from donor cells, the patient is
         | fatally vulnerable even to normally banal pathogens such as the
         | common cold. Keeping the patient isolated and alive in a
         | hospital full of germs is a major challenge.
         | 
         | That is why this kind of therapy is only used when the patient
         | faces death anyway.
        
         | sinenomine wrote:
         | >I'm squeamish about genetic research
         | 
         | And I'm sad about millions upon millions of mostly well-meaning
         | people, who died of ultimately preventable causes - if only we
         | could push the research from the lab to clinic faster.
         | 
         | Everybody is prone to ageing, and very soon it will be our
         | turn.
        
           | lettergram wrote:
           | The challenge with genetics research is it could also result
           | in the death of every human on the planet.
           | 
           | It's an amazing power for good and bad.
        
             | sinenomine wrote:
             | You don't need to ban, say, clinical trials of gene therapy
             | (especially with inert lipid nanoparticle carriers which do
             | not replicate), to ban a quite narrow and not really
             | clinically useful subfield called "gain of function
             | research".
             | 
             | Why can't our legal system deter the obvious high-level
             | publicly visible perpetrators of the recent "gain-of-
             | function" fiasco is really some food for thought.
        
               | shadowgovt wrote:
               | As a general rule, we don't arrest people for things that
               | aren't illegal.
               | 
               | ... Unless by "deter" you just meant "make it illegal,"
               | in which case, agreed.
        
               | lettergram wrote:
               | It's more than broad weaponization of viruses that are a
               | danger though.
               | 
               | Take for instance what we're doing with plants. We make
               | designer plants that provide food, and are immune from
               | particular pesticides. We then spray the fields and all
               | the other plants and bugs die (besides the immune
               | plants).
               | 
               | What happens when it turns out some genetic inserts in
               | the corn creates something like mad cow disease (but in
               | humans) 3-5 years later? Turns out some protein the corn
               | produced we thought was harmless caused massive issues.
               | 
               | Genetics has massive implications even if well meaning.
               | 
               | I think the ability to eliminate disease is massively
               | powerful and a net good. That said, I'm concerned about
               | the long term tail end risks of such work.
               | 
               | I don't think you can ban it, because someone will do the
               | work. And those that do the work will get a competitive
               | advantage (eventually letting them "win"). I think it's
               | about mitigating risks by reducing / limiting mass
               | adoption of genetic based technology without a very long
               | window of analysis.
        
               | filoeleven wrote:
               | > I think the ability to eliminate disease is massively
               | powerful and a net good.
               | 
               | One real-world example of this is genetically modifying
               | the American Chestnut by splicing in genes of the
               | Japanese Chestnut, which is resistant to the blight since
               | it co-evolved with it. The resistance comes from how
               | Japanese Chestnut trees respond to oxalis acid by walling
               | it off, preventing the fungus from spreading.
               | 
               | > That said, I'm concerned about the long term tail end
               | risks of such work.
               | 
               | One bleak science fiction novel that takes place in this
               | kind of future, specifically an amped-up version of the
               | corporate GMO food crop meddling you referenced, is The
               | Windup Girl by Paolo Bacigalupi.
        
             | BurningFrog wrote:
             | How could genetics research kill all humans?
             | 
             | Asking for a friend.
        
               | [deleted]
        
               | PicassoCTs wrote:
               | Eh, life forms can have a exponential growth curve, so
               | virologist and bacterial genetic research can easily kill
               | a large part of the population. But then we are well on
               | the way for that, so who cares?
        
               | BurningFrog wrote:
               | Developing a virus that kills a certain ethnic group
               | seems like it could be possible.
        
               | PicassoCTs wrote:
               | One could even craft a virus that is targeting a certain
               | behavioral anomaly like sociopathy, if its genetically
               | clearly identify-able. All one would need is a proof-able
               | potent carrier virus and once could reign in the
               | destructive potential of humanity.
               | 
               | "No more bad boy behavior, or the bad boys vanish from
               | the surface of the earth." behavioral deterrence via a
               | biologic weapon.
               | 
               | What a fascinating nash equilibrium, the unsocial
               | monsters, forced to be social, including applying gentle
               | pressure, so they do not become history like there
               | victims of the past. What a concept..
               | 
               | Then it mutates and kills us all..
        
               | meowface wrote:
               | >One could even craft a virus that is targeting a certain
               | behavioral anomaly like sociopathy, if its genetically
               | clearly identify-able.
               | 
               | It isn't, and I'd be surprised if it ever could be. There
               | will always be statistical correlations (especially when
               | looking at multiple genes in combination) but you can't
               | conclude what a person's brain and mind are like just
               | from their genome.
        
               | shadowgovt wrote:
               | Probably not. Genetics isn't nearly as clean a people
               | imagine it to be.
               | 
               | Much easier to target one individual (and their whole
               | host of genetic factors) than to target one genetic
               | factor that can be associated with an ethnic group and
               | pray that it doesn't either also express itself in an
               | undiscovered way in other ethnic groups or have knock-on
               | effects or the potential to mutate.
               | 
               | We aren't as genetically different as people imagine, and
               | this sort of approach would be absolutely playing with
               | fire.
        
             | epgui wrote:
             | That's wildly over-apocalyptic for such a general
             | statement. (biochemist)
        
             | hoseja wrote:
             | Nonono that would be lovely, finally some evolutionary
             | pressure. What's the real threat is breeding obligately
             | obedient citizenry and immortal leaders. Which is the first
             | thing practical genetic engineering will probably be used
             | for.
        
           | [deleted]
        
         | GenerocUsername wrote:
         | I feel like this type of medicine is more like a research study
         | waiting for a perfect match patient than it is highly
         | personalized treatment...
         | 
         | Is this technique broadly applicable? Or limited to very
         | specific cases
        
           | christkv wrote:
           | Its coming within the decade for cancer vaccines. The trick
           | is to be able to scale production and lower the turnaround
           | time.
        
           | DoreenMichele wrote:
           | It looks to me like a somewhat unique opportunity to risk it
           | because without this treatment, she was dead anyway and in
           | fairly short order. So it was a means for them to learn
           | something with very little potential downside. Worst case
           | scenario: The treatment kills her. And she was dying anyway.
           | 
           | From the article:
           | 
           |  _In Alyssa 's therapy, each of the base edits involved
           | breaking a section of genetic code so it no longer worked.
           | But there are more nuanced applications where instead of
           | switching an instruction off you can fix a defective one.
           | Sickle-cell anaemia, for example, is caused by just one base
           | change that could be corrected._
           | 
           | And I already quoted (in my previous comment) the next
           | paragraph that indicates they are already doing trials. This
           | is potentially a big deal and they seem to be pursuing it in
           | a very reasonable and conservative fashion, which is always
           | something that concerns me with this type thing. There's a
           | lot of potential for terrible and horrifying abuse and that
           | is not what I am seeing described here.
        
             | runnerup wrote:
             | Seems like this particular version of gene editing only
             | works with blood/marrow disorders? If someone had a single
             | mutation affecting their colon or eye, would the same
             | technique be appropriate?
        
               | markus92 wrote:
               | Blood/bone marrow is relatively easy as you have a very
               | clear delivery vehicle for your modified genes. Actually
               | getting to the colon cells or retina is a lot harder,
               | can't just inject it into the blood and expect it to end
               | up in the right place.
        
             | pbronez wrote:
             | Yeah, those are valuable opportunities. You have to take
             | risks to learn, but human life is to valuable to risk.
             | 
             | I remember looking into the state of the art around in
             | utero and neonatal health when we got pregnant. I was
             | astonished at how limited the knowledge is. I quickly
             | realized the big gaps are just because it's too risky to
             | study. You can't morally experiment on pregnant people and
             | infants.
        
           | gizmo686 wrote:
           | A "problem" with this treatment is that, for most patients,
           | the current standard of care works. To the extent that this
           | treatment is effective, it is likely effective for most
           | leukaemia patients, including those who would respond well to
           | current treatments. It is very plausible that this treatment
           | (or a refined version of it) will eventually be shown to be
           | superior to the current standard of care, in which case it
           | would become the standard of care, and the current treatment
           | will be relegated to the niche patient that does not respond
           | to gene edited t-cells.
           | 
           | More broadly, the technique of precisely editing DNA of
           | select cells to perform specific engineered tasks is very
           | broadly applicable. But that is a technique for developing
           | treatments, not a treatment itself.
        
             | noobermin wrote:
             | Remember what "works" means in this case. Chemotherapy, or
             | for other cancers, radiation, surgery that removes large,
             | healthy parts of organs that might have a tumor.
             | Chemotherapy has hosts of side effects, of course (hair
             | loss, nausea, ruined immunity, CIPN, etc) some permanent
             | for some people. It "works" when you look at what KPIs
             | oncologists like, very reductionist things like "5-year
             | overall survival" whilst you ignore things like quality of
             | life both in the short term and long term.
             | 
             | We should be moving away from chemotherapy. It really is
             | poison (cytotoxin) that has "treatment" of cancer as a side
             | effect, just because it will readily kill cancer cells more
             | than healthy cells whilst also taking a lot of the healthy
             | cells with it. But, it's the "established" standard of
             | care, which many doctors built their careers on, so I
             | suppose it won't go away soon.
        
               | wholinator2 wrote:
               | Surgery to remove part of an organ, followed by
               | chemotherapy gave my mother a few more years of life. Are
               | you suggesting there is some other alternative, currently
               | accepted and understood, that would've worked better?
               | Because while those side effects are terrible they are
               | usually more temporary than the extension of life that
               | they afford people. Face the question, would you rather
               | chemo for a couple months and then live a couple years,
               | or die in a couple weeks?
        
               | tpm wrote:
               | No, they are suggesting we should strive for better
               | therapies.
        
             | walkerbrown wrote:
             | > will eventually be shown to be superior to the current
             | standard of care, in which case it would become the
             | standard of care
             | 
             | How does this overturning occur? Do care providers agitate
             | for change? Or is it typically a response to headstrong
             | patients and families?
        
               | Someone wrote:
               | It starts with researchers convincing their ethical
               | review board that they should be given the opportunity to
               | experiment with the different approach.
               | 
               | That's why new procedures almost always are done on
               | patients who are as good as dead (gene editing to change
               | one's hair color won't soon get past ethical review, for
               | example, but if gene editing gets used in mainstream
               | medicine, enough data may be collected to make a review
               | board say it's acceptable to give it a try)
               | 
               | The first experiments typically only prove the medicine
               | or procedure works. Long term survival of the patient the
               | exception. From there, it's step by step towards treating
               | healthier and healthier patients.
               | 
               | If it turns out the approach is better for the typical
               | patient, patient groups (who may even have paid for part
               | of the research) start lobbying for its use. If the new
               | procedure is both better and cheaper, insurers also will
               | lobby for it.
        
             | throwuwu wrote:
             | Isn't this treatment simply accomplishing the same thing
             | that the current treatment does but in a targeted way with
             | hopefully much fewer side effects? When you have cancerous
             | T cells the only option is to eradicate all of them and
             | start over with a bone marrow transplant. Normally that
             | would mean frying them with radiation or poisoning with
             | chemo which also effects a lot of other tissue. This
             | treatment is supposed to only kill the T cells.
        
               | specialist wrote:
               | That's my noob understanding too. This is a more precise
               | form of autologous transplant.
               | 
               | I assume quick adoption of base editing for trickier
               | cases, eg for T-cell acute lymphoblastic leukemia, like
               | this patient had. Note that she had a previous
               | transplant, using the more conventional treatment of her
               | T cells. Just starting with base editing will be much
               | less onerous to future patients.
               | 
               | This is one tough person. Surviving chemo then two
               | transplants is amazing. Just thinking about it crushes my
               | heart.
               | 
               | Source: Had bone marrow transplant 30+ years ago. My then
               | experimental treatment became the default option (as
               | applicable). Don't know much about biology or medicine,
               | but I can repeat what I've heard and read.
        
           | phkahler wrote:
           | Wiping out your immune system and replacing it has something
           | like a 50/50 survival rate even if you use your own cells.
           | It's a hail Mary than usually either kills or cures.
           | 
           | I knew a guy with cancer that had this done. He survived the
           | process, but unfortunately the cells they restored him with
           | were not cancer free like they thought. He died anyway.
           | 
           | I think increasing the survival rate for these things could
           | be very useful.
        
           | l33tman wrote:
           | Wiping out the recipients immune system and transplanting a
           | donor system is in itself a common (advanced) practice since
           | long, so the new part here is the tweaking of the donor
           | system. Very interesting.
        
             | maxerickson wrote:
             | The edited cells did not replace her immune system. They
             | were used to destroy it.
             | 
             | Then after that a bone marrow transplant was done to
             | restore it.
        
       | namaria wrote:
       | We are at the "assembly" stage of genetic tinkering. Manually
       | changing over the 'machine code' of the cell, the linear sequence
       | of physically bound instructions. I wonder what the future might
       | bring in terms of higher level programming of cells.
        
       | cactusplant7374 wrote:
       | This is CRISPR, right? Or is base editing different?
        
         | jghn wrote:
         | Similar but not the same [1]
         | 
         | [1] https://www.nature.com/articles/d41586-019-03536-x
        
           | greesil wrote:
           | Sounds like the crispr "grep" part is the same, but the
           | payload does a base pair swap instead of cleaving.
        
       | once_inc wrote:
       | When is the head of the department going to do a televised
       | interview?
       | 
       | > https://youtu.be/B3xY6Ffy_wE?t=68
        
       | smeej wrote:
       | Tangential, but I wonder how long it'll be before we start
       | combining these recent advances in AI with a dataset like
       | Medikanren and identifying really custom treatments.
        
       | lebuffon wrote:
       | This is a wonderful outcome and amazing science and all involved
       | are to be commended for saving this girl's life.
       | 
       | However would it not make more sense to try and prevent the
       | disease in society, since there is a viral component to it? It is
       | a retro-virus and was first discovered in 1977. Is there even
       | research on a vaccine? We have one for cervical cancer. (Hit's a
       | nerve since I have a child with Lymphoma)
       | 
       | https://www.who.int/news-room/fact-sheets/detail/human-t-lym...
        
       | [deleted]
        
       | MontagFTB wrote:
       | It sounds like this is a continuation of work that was tested as
       | far back as 2015: https://www.businessinsider.com/r-gene-edited-
       | cells-keep-can...
        
       | dossy wrote:
       | Finally, debugging human software - this is the inevitable future
       | we've been waiting for.
        
       | janalsncm wrote:
       | Huge news. This will save tons of people as the cost comes down.
       | We are truly living in the future.
        
         | nullish_signal wrote:
        
           | hughes wrote:
           | Are you saying Alyssa's rare form of leukemia should not have
           | a cure because she and her friends in oncology might
           | quadruple the planet's population?
        
           | subroutine wrote:
           | Would you trade (a) total cure for all cancer for (b) the
           | human population to go from 8 to 16 billion?
           | 
           | Yes, absolutely. Surely population growth can be dealt with
           | more humanely than killing people off with cancer.
        
             | nullish_signal wrote:
             | I disagree with the notion of benevolent DOCTORS "Killing
             | people off with cancer".
             | 
             | Was the patient body full of DOW Chemical or DDT or Big
             | Gulps or Pharmaceuticals[0]? industrial Society and Old Age
             | are what "kill people by means of cancer"
             | 
             | [0] USA being the only country I know of to allow public
             | Advertisement of Controlled medicines
        
               | subroutine wrote:
               | I'm not sure what you're talking about. Choosing to not
               | cure cancer is killing people off with cancer. The little
               | girl in the article had leukemia. It sounds like you
               | would prefer to let children die of cancer because of
               | your weariness of population growth.
        
           | ryanwaggoner wrote:
           | I believe the world population is expected to peak this
           | century, not because of people dying or starving at higher
           | rates, but because when countries become more developed,
           | their birth rates plummet. I don't think there's any reason
           | to think curing _all_ diseases and ensuring no one ever
           | starved again would change that.
        
           | tsujamin wrote:
           | Echoing a response to a nearly invisible comment above, why
           | did make this comment?
           | 
           | These sorts of articles are a weird attractor on this site...
        
           | unlikelymordant wrote:
           | You arent anti-natalist, so your view is that people should
           | be born and die from preventable disease or starvation
           | ("medicine and agriculture saved them")? Isnt anti-natalism
           | preferable to that?
        
           | SapporoChris wrote:
           | The idea that we should not progress because we might have
           | more populace is repulsive. A simple reversal of the idea is
           | insightful. Should we minimalize the human population? Seems
           | absurd.
        
             | nullish_signal wrote:
             | Homeostasis.
             | 
             | I propose the DEFAULT be no growth or no decay. Of course
             | it's natural for animals to spread and be fertile. Of
             | course. But we already conquered and inhabited everywhere
             | with land and water.
             | 
             | Obviously Technology will advance or stall (or collapse)
             | regardless of my small whims -\o/-
        
           | FpUser wrote:
           | >"What happens when there are 16 or 32 Billion people?"
           | 
           | Not sure about other part but in the western world birth rate
           | is gong down and is not enough to offset decrease of the
           | population. Having people leave longer will not change it in
           | any way. If we ever grow to this number it will not be
           | because of curing cancer.
           | 
           | >"Even the idea that "GDP should rise 2% every year" makes me
           | nauseous."
           | 
           | You think anybody responsible for GDP growth gives a hoot
           | about what "makes you nauseous"?
        
           | Tade0 wrote:
           | Past projections of human population growth were generally
           | overblown, because nobody expected e.g. China's population to
           | (officially) peak this year.
           | 
           | Meanwhile world population growth peaked in the late 60s and
           | has been in decline ever since. The 21st century will likely
           | see the global population reaching its limit at less than
           | 11bln.
           | 
           | That's still a lot, but not nearly as much as 16bln.
        
             | PaulDavisThe1st wrote:
             | Still, it could be like saying "Falling from the 8th floor
             | is nothing like falling from the 20th", despite the fact
             | that both of them are fatal.
        
               | nullish_signal wrote:
               | If 8 or more story-falls are fatal... 8-Town limits hi-
               | rises to 8-Stories 20-Town limits hi-rises to 20-Stories
               | 
               | Which Town and what Height of Buildings would you rather
               | be a tenant of?
               | 
               | ...I don't know about Human Population, but I know that
               | "more code" is certainly not "better code", just as "more
               | sub-systems" does not mean "more quality of code"
        
               | d_tr wrote:
               | Stop embarrassing yourself. You started by talking about
               | population growth in a post about a kid's cancer, and now
               | you are continuing with making stupid analogies that
               | barely make sense.
               | 
               | Getting rid of cancer will alleviate a huge source of
               | suffering and despair and it will do nothing of
               | consequence to the population. Even if it did we should
               | not care.
        
           | dools wrote:
           | There will only be 11b people
        
           | zamfi wrote:
           | Don't worry, there's no shortage of ways to prevent getting
           | to 16 or 32 billion people.
           | 
           | First among those is that very few countries have birth rates
           | that exceed replacement rates! And the others are on a very
           | strong trend to follow over the next 10-20 years.
        
           | gizmo686 wrote:
           | A) We have already largely solved infant mortality in the
           | developed world, and are making progress on the issue
           | globally. Societies seem to adapt over time by decreasing
           | birthrates to compensate [0]. In fact, in most of the
           | developed world, birth rates are below replacement rate.
           | 
           | B) Despite all of the medical advances we have made, we have
           | hardly put a dent in old age. Medical science has made great
           | progress in stopping people from dieing young, which has
           | pushed up the average lifespan. But a 100 year old today is
           | still likely to die within a few years even with the most
           | advanced medical treatment.
           | 
           | Having said that, at some point, we are likely to start
           | making breakthroughs in maximum lifespan, which could very
           | quickly put us back into rapid population growth. Eventually,
           | we would probably see society adjust to the point that having
           | _any_ children is no longer expected of a typical human, but
           | that could be a painful transition depending on how quickly
           | we we go from a lifespan of ~100 to unbounded.
           | 
           | [0] Although it is not clear if this is directly related to
           | infant mortality, or a response to broader economic changes,
        
             | tluyben2 wrote:
             | Where I live in the EU, people get old; 100 here is not so
             | rare and there are many 90+. Most of them are healthy;
             | walking the mountain for shopping daily and going to the
             | bar for a shot or two in the morning. 0 of them want to
             | live longer. Not because physical but because the mental
             | agony of yet more time, see more horror. Think people who
             | don't have this issue underestimate how bored and 'done'
             | most older people are. My grandparents (all 4) made close
             | to 100 in good health and wanted out since 70. When you are
             | young you want to live forever, when older I don't think
             | many people do.
        
             | Aeolun wrote:
             | I seem to remember reading that there is some limit to the
             | age of the brain, separate from the mortality of the body.
             | So even if you were to not die from any cause you'd be
             | pretty much done after 150-170 years.
        
               | delecti wrote:
               | I think it's a stretch to assume we know much about what
               | happens to brains after just 120, let alone 150-170.
        
               | gizmo686 wrote:
               | Setting aside any specific mechanism, the overall point
               | still seems plausible. Assuming we can prevent the
               | primary causes of aging (e.g. telomer shortening), we
               | will likely start to find other parts of human biology
               | that start to fail, either because of additional aging
               | mechanisms, or just limitations of the body's self-repair
               | mechanisms.
               | 
               | Human biology is incredibly complex, and there has been
               | no evolutionary pressure for it to sustain itself beyond
               | its natural biological clock. These problems are probably
               | solvable eventually, but not until we live long enough to
               | see them. Which problems means we will have at least a
               | few generations of people die after the initial old-age
               | cure.
        
             | nullish_signal wrote:
             | The guesses at what culture might look like in a fully-
             | populated planet, beyond the reduction of birth rates, is a
             | very interesting consideration.
        
             | nullish_signal wrote:
             | I must add, a reply to my moaning about limitless
             | exponential human growth that starts off with
             | 
             | "We have already largely solved infant mortality in the
             | developed world, and are making progress on the issue
             | globally."
             | 
             | ...certainly makes me feel feelings about population
             | growth!!
        
               | Dylan16807 wrote:
               | The immediate feelings you had before you read the second
               | sentence are not good conversational material.
        
               | tluyben2 wrote:
               | You have a lot of feelings about stuff that is. As I get
               | older, that seems incredibly unproductive and I stopped
               | doing that.
        
           | bglazer wrote:
           | You first
        
           | ekianjo wrote:
           | Better to have more people than to be on the down slope of
           | demography where the world world ages faster and faster
        
           | mmsc wrote:
           | I thought the same thing initially, then I realized that rare
           | forms of cancer are... rare. Not that many people die from
           | them to cause a significant increase (or lack of decrease) in
           | the population.
        
           | tptacek wrote:
           | You can't simultaneously be pro-natalist and anti-
           | exponentialist, at least not without being a Bond villain.
        
       | daniloalmeida wrote:
       | I just read the title to get hopeful. Now I'm in the comments to
       | be realistically disappointed.
        
       | klipt wrote:
       | > The team at Great Ormond Street used a technology called base
       | editing, which was invented only six years ago.
       | 
       | Fun fact: J M Barrie who wrote Peter Pan donated the copyright of
       | both the book and play in perpetuity to Ormond Street children's
       | hospital. It's been a significant source of funding for them over
       | the years.
        
         | Semaphor wrote:
         | > It's been a significant source of funding for them over the
         | years.
         | 
         | TFA:
         | 
         | > Still, best estimates are that it's not a hugely important
         | part of the hospital's budget.
         | 
         | which links to The Guardian [0]:
         | 
         | > The hospital has abided by Barrie's wishes expressed in his
         | will and always kept the proceeds from Peter Pan a closely
         | guarded secret. With copyright expiry looming, De Poortere is
         | at pains to point out the revenues are "significant", but not
         | as great as many suspect.
         | 
         | > "People who talk about millions a year have a strange idea of
         | what a character is worth," she says. "On the whole, it's been
         | significant but I wouldn't call it huge. I am on my own doing
         | the Peter Pan administration, but I am part of a charity that
         | has 100 people."
         | 
         | > If the newspapers of 1929 were right about proceeds of
         | PS2,000 a year, that would be PS90,000 a year in today's money,
         | according to the Office for National Statistics.
         | 
         | Just to expand ;)
         | 
         | [0]:
         | https://www.theguardian.com/business/2007/dec/28/gtormondst
        
         | monero-xmr wrote:
        
           | ShamelessC wrote:
           | what in the actual fuck
        
           | sgc wrote:
           | You need to avoid spending the principal + inflation, which
           | is significantly harder to do.
        
           | hilbertseries wrote:
           | Why did you make this comment?
        
             | monero-xmr wrote:
             | Regarding donations - you should donate assets that will
             | produce cash for infinite time. Pure cash donations that
             | are unencumbered are foolish, IMO.
        
             | jacobriis wrote:
             | AI that filters insane non-sequitur personal anecdotes from
             | HN comments cannot come soon enough.
        
               | sph wrote:
               | Leave AI out of forums for humans, please. They are not
               | welcome here.
        
             | guiambros wrote:
             | ChatGPT?
        
         | hn_throwaway_99 wrote:
         | Thanks very much for posting this tidbit, very interesting!
         | 
         | After seeing your comment I did a little googling, and
         | fascinating to me is that a special law was passed in the UK
         | specifically to make one of the Peter Pan copyrights held by
         | Great Ormandy Street never expire:
         | https://www.plagiarismtoday.com/2015/10/21/peter-pan-and-the...
        
           | jv22222 wrote:
           | Huh, very cool. I had never imagined there would be a
           | scenario where I was genuinely happy about a never ending
           | copyright!
        
             | Dylan16807 wrote:
             | Keeping some stories out of the public domain forever is a
             | pretty terrible way to fund something.
        
               | [deleted]
        
               | eyelidlessness wrote:
               | Don't worry, Pan never wanted to grow up.
        
               | sph wrote:
               | I dunno, if it saves some children's lives, instead of
               | Disney trying to make it another blockbuster franchise,
               | that's perfectly fine by me.
        
               | bambax wrote:
               | Taking away freedom is always about "saving children's
               | lives". It's so common in fact that the instant one hears
               | about "saving children's lives" we can be certain
               | something very bad is going to happen.
        
               | johngalt_ wrote:
               | I am not a big fan of copyright myself, but calling being
               | unable to use someone else's story for yourself "taking
               | away your freedom" is a bit too much.
        
               | bambax wrote:
               | Isn't it obvious where this could be going? Let's save
               | more children's lives by making all copyright infinite.
        
               | foldr wrote:
               | I mean, no? There is absolutely no indication that that
               | particular slippery slope is being slid down. Can you
               | even point to anyone advocating for infinite copyright on
               | all works on the basis of the Great Ormond Street
               | example?
        
               | Dylan16807 wrote:
               | Not if you really believe in the concept of public
               | domain. Old stories should belong to everyone, not
               | "someone else".
        
               | pastacacioepepe wrote:
               | The proper solution would have been for the state to fund
               | the hospital as needed, while freeing everyone else to
               | create content based on Peter Pan if they wished so.
        
               | namaria wrote:
               | Civilization is made of unsavory compromises... Funding
               | by taxation and committee spending, or by fetishisation
               | of conspicuous consumption are also pretty bad and yet
               | quite prevalent.
        
               | jonsen wrote:
               | Agree, there should be other ways in a modern society.
        
               | [deleted]
        
             | musicale wrote:
             | In this case the copyright seems to have expired
             | essentially everywhere, but a special UK extension for
             | Peter Pan allows for royalty collection for performances or
             | adaptations of the play there in spite of the copyright's
             | expiry.
        
       | jordanbeiber wrote:
       | I've started watching star-trek (as it makes me fall asleep
       | immediately) - this stuff sounds like a star-trek episode!
       | 
       | Something Dr Crusher would come up with.
       | 
       | It makes a distant future where we quickly engineer a fix and
       | inject it seem almost plausible.
        
         | rmak wrote:
         | Closer to star gate sg1 episode than star trek, I love that
         | series.
        
         | unsupp0rted wrote:
         | It is something Dr. Crusher came up with:
         | 
         | > Barclay's Protomorphosis Syndrome
         | 
         | > Attempting to cure Lieutenant Reginald Barclay of his
         | Urodelan flu, Dr. Crusher used synthetic T-cells to activate
         | the gene that would fight off the infection. However, an
         | anomaly in Barclay's genetic structure caused the T-cell to
         | mutate and activate all of Barclay's dormant genes, including
         | his introns. The T-cells then became airborne, spreading
         | throughout the ship like a virus and causing the crew to "de-
         | evolve."
         | 
         | https://memory-alpha.fandom.com/wiki/Barclay%27s_Protomorpho...
        
           | yread wrote:
           | wow it goes from ok to completely nuts within one sentence.
           | Activated introns hahaha
        
         | namaria wrote:
         | Oh man I love getting high and watching the original Star Trek.
         | All the imagined analogue computer technology makes me
         | wonder...
        
         | system2 wrote:
         | Dr. McCoy would've given green alcohol.
        
       | ars wrote:
       | Is there any worry/chance that the modified T-cells will survive
       | and multiply in the body instead of dying out?
        
         | gizmo686 wrote:
         | I'm talking well beyond my competency, but short of one of
         | those T-cells spontaneously becoming cancerous itself, I don't
         | see how. T-cells do not duplicate themselves. Instead, new
         | T-cells are creates in bone marrow. Since they are not editing
         | the genes in the bone marrow, there is no mechanism within the
         | body to create new cells using the modified DNA.
        
       | hliyan wrote:
       | Great to see medical techniques slowly starting to resemble
       | engineering design:
       | 
       | 1. Donor T-cells added with receptors to destroy the recipient's
       | cancerous T-cells.
       | 
       | 2. Donor T-cells' markers removed to prevent them from attacking
       | each other.
       | 
       | 3. Donor T-cells' existing receptors removed to prevent them from
       | attacking the recipient's other cells (since the donor T-cell
       | will see them as 'foreign').
       | 
       | 4. Donor T-cells altered to resist chemotherapy.
       | 
       | It seems each of these changes were achieved by editing specific
       | base pairs to _break_ the relevant gene, except perhaps for (1)
        
         | adamredwoods wrote:
         | To even have something like this actually respond, in the way
         | they planned it, is a marvel. I know they have worked with
         | T-Cells before, but this is putting it to precise use.
         | 
         | Sadly, cancer is vicious. It only takes one rogue cell to keep
         | building, about 2-years for it to surface again. Luckily, this
         | process seems repeatable.
         | 
         | I can't imagine the euphoria that family feels right now.
        
       | beaned wrote:
       | Do people with base-edited DNA pass their modified genes on to
       | their offspring?
        
         | elektrontamer wrote:
         | Germ cells need to be edited for that. The edited T cells can't
         | get inherited.
        
         | gizmo686 wrote:
         | No. The only DNA that gets passed to offspring is what is in
         | the sperm/eggs. Collectively these are referred to as the
         | "germline". Research into germline editing is illegal in the
         | US, and frowned upon internationally.
         | 
         | The most famous case is from 2018, when Chinese researcher He
         | Jiankui created the first genome-edited babies. Using CRISPR to
         | give the babies resistance to HIV-1. [0]. This change will
         | propagate to the decedents of the subjects [1]. The Chinese
         | Academy of Science, along with the global medical community,
         | denounced this experiment; and He Jiankui was arrested.
         | 
         | [0] The necessary mutation, while rare in humans, is naturally
         | occurring, and generally thought to confer HIV-1 resistance.
         | 
         | [1] Subject to normal genetics that arrises from sexual
         | reproduction, where a given parent is responsible for only half
         | of a child's genes.
        
           | NaturalPhallacy wrote:
           | > _Research into germline editing is illegal in the US, and
           | frowned upon internationally._
           | 
           | I would bet money someone's doing it anyway.
        
       | hooloovoo_zoo wrote:
       | Marvelous! I wonder why she needs a second transplant though.
        
         | Izikiel43 wrote:
         | The base therapy basically removed her immune system, the
         | second transplant recreated it.
         | 
         | In software terms, they did a clean install of the immune
         | system after formatting it.
        
           | uwagar wrote:
           | treating biology as software isnt gonna end well.
        
         | t-3 wrote:
         | It's explained in the article that the second transplant is
         | necessary to replace the white blood cells which should have
         | been fully wiped out by the first transplant of modified cells.
        
           | b800h wrote:
           | But her body ought to start manufacturing her original
           | T-cells at some point, right? Or are the stem cells producing
           | them cancerous too? Doesn't say that in the article, and it
           | doesn't seem like a reasonable inference, so there's a bit of
           | detail missing there.
        
             | sowbug wrote:
             | Even if the self-manufactured cells were healthy, the
             | patient is vulnerable to infection with a compromised
             | immune system, and adding more white blood cells reduces
             | that vulnerability.
             | 
             | Tangent: before undergoing some kinds of chemotherapy,
             | patients are injected with drugs that stimulate white-
             | blood-cell production. Most of your body's WBC production
             | happens in the long bones of your body, and the sudden
             | burst of "factory" activity makes your legs ache, the way
             | growing pains hurt in children.
        
           | hooloovoo_zoo wrote:
           | But why can't the modified cells function as regular T cells
           | in addition to the modifications?
        
             | zaroth wrote:
             | I think they don't usually last that long. The body won't
             | keep producing the modified cells.
             | 
             | Plus I think the modification made them basically only good
             | for killing the native (cancerous) T cells
        
               | hooloovoo_zoo wrote:
               | Could be. It would be nice if you could maintain the
               | modification though because then you don't have to worry
               | about some cancerous cells surviving the first wave.
        
             | frontierkodiak wrote:
             | The article states "The first base edit disabled the
             | T-cells targeting mechanism so they would not assault
             | Alyssa's body". Not an expert but I presume the 'targeting
             | mechanism' is the T-cell receptor, whose activation is
             | necessary (but not sufficient) to trigger immune response.
        
               | hooloovoo_zoo wrote:
               | Maybe, but the second transplant also needs to not
               | assault Alyssa.
        
               | Narretz wrote:
               | The second transplant is a stem cell (bone marrow)
               | transplant, i.e. it replaces the cells that produce the
               | t-cells.
               | 
               | This is how leukemia therapy is normally finished after
               | chemotherapy / radiation therapy kills the cancer cells.
        
       | ggm wrote:
       | In heroic medical science history fiction it's a bit of a trope
       | to write "the operation was a success but the patient died"
       | 
       | I sincerely hope she survives at least 5 years, and significantly
       | more. If she gets only a single year of treatment -free high
       | quality life outside of respite care, that would be a bitter pill
       | outcome but might still be held a success. Sometimes giving
       | people a year of quality life at end-of-life is a good outcome.
       | 
       | The article as written is light on for data and time info. She's
       | had at least 3 post treatment checks one of which was 3 months,
       | the other 2 not stated but 6monthly might not be unusual.
       | 
       | Recurrences in blood borne diseases are not unheard of. She had
       | some signs of recurrence at 3 months but it went away. Maybe
       | fragment traces? Non viable remainders flushing out of the
       | marrow? (I don't know if this is even plausible. I'm not in
       | medicine)
       | 
       | It's great news, but so was Christian Barnard's first transplant.
       | In modern terms the survival wasn't very long. (200 days in the
       | first 4)
       | 
       | A better outcome would be like Steptoe's work on assisted
       | conception and birth. (IVF) and I certainly hope she and future
       | patients have that.
        
         | ausbah wrote:
         | is there any specific reason to think this disease could come
         | "roaring back" aside from general unknowns of a new treatment?
        
           | epgui wrote:
           | The fact that she developed cancer in the first place means
           | that she is more likely to continue developing cancer, via a
           | plethora of mechanisms. Cancer is always just a matter of
           | time, for all of us. Subtle mutations accelerate the
           | timeline, and they accumulate over time.
        
       | uwagar wrote:
        
         | thdespou wrote:
         | Noone is forcing you to accept anything. If else the girl and
         | parents had to make that decision and accept the risks. They
         | would probably had to sign a legal document as well. Maybe you
         | need to chill out of the conspiracy mindset.
        
           | uwagar wrote:
           | lol. said the same thing about covid vaccine and then brought
           | in the mandates.
        
       | Doorstep2077 wrote:
       | This looks pretty great, hopefully a huge step forward for the
       | medical industry and finding cures for cancer. Unfortunately
       | don't know too much about it; wish there was something that
       | allowed me to learn about medical problems easily, without
       | needing to understand the jargon
        
       | ricardobeat wrote:
       | Very similar report by the same author from 2015:
       | https://www.bbc.com/news/health-34731498
       | 
       | It doesn't explain what "designer immune cells" were, so it's
       | hard to tell what's different in this more recent treatment.
        
         | Duller-Finite wrote:
         | that study used TALENs; this one used CRISPR base editors
        
       | thewatcher23 wrote:
        
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