[HN Gopher] Some Proteins Change Their Folds to Perform Differen...
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       Some Proteins Change Their Folds to Perform Different Jobs
        
       Author : pseudolus
       Score  : 86 points
       Date   : 2021-02-04 11:53 UTC (11 hours ago)
        
 (HTM) web link (www.quantamagazine.org)
 (TXT) w3m dump (www.quantamagazine.org)
        
       | chromanoid wrote:
       | I don't like the headline. As if the protein decides to do so.
       | Maybe this sounds a little bit less "active" but probably also
       | less sensational: "Some Proteins Perform Different Jobs By
       | Changing Their Folds"
        
         | natechols wrote:
         | One of my pet peeves is science journalism abusing the active
         | voice, often in ways that almost anthropomorphize the process.
         | The absolute worst are phrases like "Nature designed this
         | protein to...", which I have seen otherwise reasonable,
         | rational writers toss about carelessly. (It's like the inverse
         | of the passive voice used to describe police shootings: "the
         | suspect was hit by gunfire", etc.)
        
           | 0_____0 wrote:
           | A reasonable reader will implicitly understand that the
           | proteins have not, in fact, acquired sentience.
        
             | blix wrote:
             | This is what I tell myself every time I read an HN comment
             | written by a collection of proteins.
        
               | nate_meurer wrote:
               | I don't normally meta-comment on other comments, but this
               | one is superb. It took me a moment to soak it in.
        
             | natechols wrote:
             | True! Problem is, there are a lot of unreasonable readers
             | too, many of them having strong opinions about natural
             | versus directed processes.
        
               | passivate wrote:
               | Well, you can't reason with unreasonable people :) That's
               | the actual problem. Also, I strongly disagree with your
               | view that a lot of them are reading scientific articles.
               | The root problem is lack of science education/curiosity
               | which needs to be addressed specifically, rather than
               | forcing the rest of the world to adopt certain phrasing.
               | 
               | Personally I like the romanticism/poetic style, it makes
               | articles seem less bland, which makes me want to read
               | them even more.
        
             | uoaei wrote:
             | Based on my experience with otherwise reasonable readers
             | who are relatively naive when it comes to biology, this is
             | not the case.
        
       | jostmey wrote:
       | Natural selection may not select for simplicity. It may not try
       | to reduce dependencies. A gene can depend on thousands of other
       | genes. And a gene can carry out multiple functions
        
         | macksd wrote:
         | Natural selection doesn't "select" for anything except the
         | survival and continuation of species. Everything else that it
         | _tends_ to favor is merely a correlation with that - so you
         | have to expect a certain level of variation and exception.
        
         | thaumasiotes wrote:
         | > It may not try to reduce dependencies.
         | 
         | This one's pretty straightforward; natural selection has two
         | very common approaches to dependencies:
         | 
         | 1. If the presence of something is not a reliable feature of
         | the environment, dependence on that something will be
         | ruthlessly weeded out.
         | 
         | 2. If the presence of something is a reliable feature of the
         | environment, dependence on that something will be created and
         | grow to permeate most existing systems.
         | 
         | The first effect has a lot of popular awareness; the second
         | doesn't. They're both important.
        
           | cmpb wrote:
           | Counterargument to 2: the air is largely nitrogen, but humans
           | do not utilize it during respiration. Thus, this reliable
           | feature of the environment has not been selected as a
           | dependency.
           | 
           | I'm not necessarily arguing it's untrue, just not quite the
           | hard-and-fast rule that 1 is.
        
             | thaumasiotes wrote:
             | > I'm not necessarily arguing it's untrue, just not quite
             | the hard-and-fast rule that 1 is.
             | 
             | This is pretty subjective. If you grow up with sufficient
             | dietary iodine, that's worth 10-15 IQ points (a gigantic
             | amount) compared to the counterfactual. Iodine
             | supplementation is highly effective in populations that
             | have presumably been going without for, at the very least,
             | many hundreds of years. This is the reason we iodize salt.
             | 
             | With that in mind, how much of a hard-and-fast rule is #1?
             | 
             | The other side of this argument is that dependencies can be
             | surprisingly subtle. This is pure speculation, but the
             | thing that leaps out at me about atmospheric nitrogen is
             | that oxygen is extremely corrosive. If the atmosphere were
             | more oxygen and less nitrogen, lungs of today might
             | experience a lot more wear and tear than they are currently
             | designed for. (Or not! I really have no idea. But I think
             | the hypothetical has some thought-experiment value anyway.)
        
             | throwaway2245 wrote:
             | Humans are systemically dependent on nitrogen in the air,
             | in that it is required for nitrogen fixation.
        
             | fabian2k wrote:
             | How should we use nitrogen gas in the air? Oxygen is useful
             | to us for energy production. Nitrogen we get from easier
             | sources in different forms.
        
           | jostmey wrote:
           | > 1. If the presence of something is not a reliable feature
           | of the environment, dependence on that something will be
           | ruthlessly weeded out.
           | 
           | Something is only weeded out if it is sufficiently
           | _disadvantageous_. There are lots of examples in biology
           | where mutations are neutral and allowed to accumulate. There
           | is also a lot of junk DNA, and even though we 've found that
           | some junk DNA was misclassified, there still is a lot of junk
           | DNA in each genome
        
             | thaumasiotes wrote:
             | Dependence on something that isn't there is disadvantageous
             | by the definition of "dependence". I don't see the point
             | you're trying to make.
        
           | sooheon wrote:
           | Interesting. Any examples of the 2nd?
        
             | jcims wrote:
             | Honeybees, sunlight, water, oxygen, etc.
             | 
             | Edit: Also -
             | https://en.wikipedia.org/wiki/Radiotrophic_fungus
        
               | matheusmoreira wrote:
               | Another awesome example:
               | 
               | https://en.wikipedia.org/wiki/Nylon-eating_bacteria
        
             | londons_explore wrote:
             | In mammals, the dependance on a certain body temperature.
             | 
             | The fact that it is regulated to a margin of 0.3% shows
             | quite how important it is. Increase or decrease the body
             | temperature by 5% (on an absolute scale, so Kelvin), and
             | you will be a dead human...
        
               | JPLeRouzic wrote:
               | A question from a layman: Could changing body's
               | temperature by a few percent, for a small amount of time,
               | be used to heal from some diseases, like cancer or
               | diabetes?
        
               | thaumasiotes wrote:
               | Some diseases, yes; people do this all the time and it is
               | called "running a fever". Fevers are not induced by the
               | disease; they are a strategy you use to kill the disease.
               | 
               | Cancer or diabetes, no. Diabetes is not even a "disease"
               | in the sense of an organism that can be alive or dead.
        
               | JPLeRouzic wrote:
               | Thanks for the answer!
        
               | thaumasiotes wrote:
               | > The fact that it is regulated to a margin of 0.3% shows
               | quite how important it is.
               | 
               | This is an excellent point. It can be applied to a huge
               | number of biological phenomena, but it almost never is.
               | 
               | An example where this idea actually has made it into the
               | mainstream is body weight. I read an article that pointed
               | out that, yes, we're fatter than we used to be, but
               | regulating body weight by measuring energy intake and
               | expenditure is doomed to fail. Articles making this claim
               | are a very rich genre, but I found the particular
               | argument fascinating:
               | 
               | People routinely maintain the same body weight, within
               | say 5 pounds, for years at a time. This is a level of
               | accuracy that we do not have the technology to achieve by
               | using measurements of energy inflow and outflow -- we
               | can't measure dietary energy that precisely! (And modern
               | technology is, by most standards, _very precise_.)
               | 
               | Thus, there is a very strong suggestion, based on this
               | incredible stability, that your body is regulating itself
               | to a certain weight. If you adjust energy intake, or
               | outflow, other things will adjust accordingly; those are
               | unlikely to be primary determinants of the system's
               | behavior.
        
           | adrian_b wrote:
           | Yes indeed, there are examples of dependencies that have been
           | removed by evolution.
           | 
           | For example cobalt and nickel were both essential for the
           | first living beings. However, on the continents they are much
           | less accessible than in the oceans.
           | 
           | Because of that, most terrestrial plants have lost the
           | dependency on cobalt (which is why they cannot provide
           | vitamin B12 to those who eat them) and most have also lost
           | the dependency on nickel (except that for many terrestrial
           | plants nickel, while no longer strictly required, can still
           | be useful by enabling them to use urea as a source of
           | nitrogen, when other sources of nitrogen are scarce).
        
           | hamilyon2 wrote:
           | Inevitable becomes necessity.
        
           | throwaway2245 wrote:
           | In my opinion, the second is just not true.
           | 
           | If a reliable feature is evolutionary advantageous, then new
           | systems will come about to take advantage of that (and may
           | out-compete old systems, indeed, to the point of extinction),
           | I agree.
           | 
           | But, if a reliable feature isn't evolutionary advantageous,
           | it can be ignored or even removed.
           | 
           | Negative features may need to be managed, rather than taken
           | advantage of. Examples: ionizing radiation, landfill.
        
       | [deleted]
        
       | jugg1es wrote:
       | I feel like I remember hearing this as an undergrad neurobiology
       | major 15 years ago. Is this even new?
        
         | dnautics wrote:
         | it's just a slightly more extreme form of the glove-and-hand
         | principle (versus lock and key). Of course even this turns out
         | to be a spectrum. there are enzymes/protein that are more lock-
         | and-key, and proteins that interact very heavily. One extreme
         | is insulin receptor (which totally mangles insulin out of shape
         | on binding). IIRC, another interesting one is antibody
         | evolution, as B cells mature they go from producing antibodies
         | that are gloves-in-hand to antibodies that are lock-and-key, as
         | measurable using IR spectroscopy:
         | https://www.pnas.org/content/103/37/13722.short
        
       | Rochus wrote:
       | Yet another challenge for AlphaFold.
        
       | hprotagonist wrote:
       | After long study, my iron-clad law of biology is this: every
       | _other_ iron-clad law of biology ends with the phrase "well,
       | except when ..."
        
         | tejtm wrote:
         | This. Decades ago I once innocently noted
         | Biology is a system of exceptions!
         | 
         | still hear the echos.
        
         | infogulch wrote:
         | This seems to apply to your law as well.
        
           | hprotagonist wrote:
           | isn't it great?
        
         | grishka wrote:
         | As a software developer, I find biology to be fascinating but
         | at the same time extremely complex and counterintuitive.
         | Everything seems to be influencing everything else in subtle
         | ways. You can never separate an organism into distinct modules
         | that do distinct jobs and expose distinct interfaces, like you
         | could a program or a hardware device when reverse engineering.
         | The fact that physics and chemistry are being exploited in most
         | unexpected ways doesn't help either.
         | 
         | But then the thing to remember is that biology is alien
         | technology in the sense that it wasn't created by humans and is
         | still ahead of our current technological progress. It's the
         | only alien technology we've been able to get our hands onto.
        
           | WJW wrote:
           | Mother Nature only cares about energy efficiency and
           | evolutionary fitness. If that means every variable has to be
           | global, so be it. She is not coding with understandability in
           | mind.
        
         | nobodyknowsyoda wrote:
         | I'm probably preaching to the choir, but yup that inherent
         | uncertainty seems to distinguish biology from the other
         | sciences. There is a huge stochasticity & serendipity in
         | everything because there is no intentionality in the design of
         | any biological component; any convergence toward a chemically
         | or physically optimized component or behavior is driven by
         | evolution by natural selection but remains imperfect. Warts,
         | quirks, and all
         | 
         | It also explains how the folks on HN positing "Covid is just
         | the flu" and "long Covid isn't real" have been so confident yet
         | so gravely mistaken. They are used to other sciences where
         | there is much less room for uncertainty
        
           | hprotagonist wrote:
           | Yep. For better or worse i've been up to my eyeballs in "hey,
           | you know, biological systems are actually insane!" since high
           | school, and now through a few advanced degrees :)
           | 
           | I've been working in tech more than normal for a few years
           | now, and the endemic nature of "the andy grove fallacy" among
           | my coworkers has ceased to startle me, but it's just kind of
           | bothersome every time.
           | 
           | https://blogs.sciencemag.org/pipeline/archives/2007/11/06/an.
           | ..
        
             | nitrogen wrote:
             | Despite the common trope that software/hardware engineers
             | think they understand everything, automation can make
             | exponential development possible in bio. I know someone who
             | works at a biotech company, and the stories I hear lead me
             | to believe that traditional thinking in e.g. medicine and
             | insurance, and from colleagues, really is a bottleneck.
             | Maybe exponential growth will never apply to the number of
             | diseases cured, but it can and should apply to the hardware
             | and software that facilitates the next iteration of
             | biological discovery.
        
               | hprotagonist wrote:
               | It's not that software/hardware engineers think they
               | understand everything, it's that the absolutely common
               | idea "my way of thinking about understanding things is
               | appropriate in this other domain" breaks down very fast.
               | 
               | I'm not speaking about running hospitals or dancing with
               | insurance companies. I'm speaking about much earlier in
               | the pipeline: fundamental, blue-sky biological research
               | is fundamentally different from software. The reason is
               | that we are not studying designed systems; the effect is
               | that there is so much that we don't know that we don't
               | know that things that sound easy are in fact basically
               | impossible because the prior knowledge is simply not
               | established. (Until they aren't. When does that change?
               | First slowly, then all at once.)
               | 
               | As a biomedical engineer, i'm on board with hardware and
               | software improvements: it's kind of my job. The trick is
               | knowing what you're doing, what you aren't, and what you
               | can expect, and to balance confidence in the value of
               | what you _can_ tightly constrain and design versus
               | humility in accepting that the natural world simply
               | doesn't care.
        
               | natechols wrote:
               | I think what bugs me the most is the common assumption
               | that we just need better modeling tools to make a lot of
               | the messy lab work go away. Everyone who raises this idea
               | seems to think it's original and revolutionary and a no-
               | brainer, but there's at least 40 years of bitter
               | experience in biotech and pharma development saying
               | otherwise. Even genuinely impressive feats like AlphaFold
               | get inflated wildly in importance (and used to
               | retroactively bash experimentalists for all the time they
               | wasted by not listening to software people). Actually
               | speeding up the entire process of biomedical research
               | requires improvements across the board in many different
               | fields, and it's not something that is magically going to
               | be solved by computer science wizardry alone.
        
               | hprotagonist wrote:
               | I primarily do modeling, and I am first in line to tell
               | anyone who is willing to listen that the very first thing
               | you should do to learn to be a good modeler is spend a
               | year at the bench first in the problem area you want to
               | model.
               | 
               | Wet lab is so informationally dense in terms of personal
               | knowledge that it's not even funny.
        
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