https://www.jstage.jst.go.jp/article/pjab/100/9/100_pjab.100.035/_article Toggle navigation [J-STAGE_lo] J-STAGE home [icon-close] * Browse + # + All titles + All subject areas + All publishers + Search articles + Search J-STAGE Data # * About J-STAGE + # + Overview + Services and features + Public data + Terms and Policy * News and PR + # + News + Maintenance information + Special contents + Media resources * Support + # + User manuals + Browser compatibility + FAQ + Contact + Sitemap * Sign in * * Cart [icon-cart] * EN [icon-lang] + # + English + Ri Ben Yu * Browse + # + All titles + All subject areas + All publishers + Search articles + Search J-STAGE Data # * About J-STAGE + # + Overview + Services and features + Public data + Terms and Policy * News and PR + # + News + Maintenance information + Special contents + Media resources * Support + # + User manuals + Browser compatibility + FAQ + Contact + Sitemap * Sign in * * Cart * EN + # + English + Ri Ben Yu * * [ ] Advanced search Proceedings of the Japan Academy, Series B Online ISSN : 1349-2896 Print ISSN : 0386-2208 ISSN-L : 0386-2208 * Journal home * All issues * Special Issue * About the journal * J-STAGE home * / * Proceedings of the Japan Acade ... * / * Volume 100 (2024) Issue 9 * / * Article overview Original Article Incorporation of photosynthetically active algal chloroplasts in cultured mammalian cells towards photosynthesis in animals Ryota AOKI, Yayoi INUI, Yoji OKABE, Mayuko SATO, Noriko TAKEDA-KAMIYA , Kiminori TOYOOKA, Koki SAWADA, Hayato MORITA, Baptiste GENOT, Shinichiro MARUYAMA, Tatsuya TOMO, Kintake SONOIKE, Sachihiro MATSUNAGA Author information * Ryota AOKI ORCID https://orcid.org/0009-0009-2340-8379 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Yayoi INUI ORCID https://orcid.org/0009-0002-4405-4783 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Yoji OKABE ORCID https://orcid.org/0000-0002-5598-0953 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Mayuko SATO ORCID https://orcid.org/0000-0002-9876-5612 RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. * Noriko TAKEDA-KAMIYA ORCID https://orcid.org/0000-0002-3019-7735 RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. * Kiminori TOYOOKA ORCID https://orcid.org/0000-0002-6414-5191 RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. * Koki SAWADA Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Hayato MORITA Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Baptiste GENOT ORCID https://orcid.org/0000-0001-9070-1772 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Shinichiro MARUYAMA ORCID https://orcid.org/0000-0002-1128-5916 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. * Tatsuya TOMO ORCID https://orcid.org/0000-0002-9369-1941 Graduate School of Science, Tokyo University of Science, Tokyo, Japan. * Kintake SONOIKE ORCID https://orcid.org/0000-0002-5327-473X Faculty of Education and Integrated Arts and Sciences, Waseda University, Tokyo, Japan. * Sachihiro MATSUNAGA Corresponding author ORCID https://orcid.org/0000-0003-3024-3559 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan. Corresponding author ORCID Keywords: chloroplast, algae, cultured mammalian cell, photosynthesis , synthetic biology JOURNAL OPEN ACCESS FULL-TEXT HTML 2024 Volume 100 Issue 9 Pages 524-536 DOI https://doi.org/10.2183/pjab.100.035 [articlelin] Browse "Advance online publication" version Details * Published: 2024 Received: August 10, 2024 Available on J-STAGE: November 11, 2024 Accepted: October 01, 2024 Advance online publication: October 31, 2024 Revised: - * Correction information Date of correction: November 16, 2024 Reason for correction: Correction of appearance of keywords in Japanese version Correction: Bibliography Details: Appearance of keywords in Japanese version * Funder information 1.Fund name: Japan Society for the Promotion of Science Fund/Funder ID: Crossref Funder ID: http://dx.doi.org/10.13039/501100001691 Grant/Award number: JP20H03297 JP22H00415 JP21K06101 JP22H02651 JP23H04961 JP22H04926 JP23H04962 2.Fund name: Japan Science and Technology Agency Fund/Funder ID: Crossref Funder ID: http://dx.doi.org/10.13039/501100002241 Grant/Award number: JPMJCR20S6 JPMJSP2108 Full-text HTML Download PDF (2994K) Download citation RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks) BIB TEX (compatible with BibDesk, LaTeX) Text How to download citation Contact us Article overview * Abstract * References (48) * Figures (6) * Content from these authors Share * * X * * [men] Abstract Chloroplasts are photosynthetic organelles that evolved through the endosymbiosis between cyanobacteria-like symbionts and hosts. Many studies have attempted to isolate intact chloroplasts to analyze their morphological characteristics and photosynthetic activity. Although several studies introduced isolated chloroplasts into the cells of different species, their photosynthetic activities have not been confirmed. In this study, we isolated photosynthetically active chloroplasts from the primitive red alga Cyanidioschyzon merolae and incorporated them in cultured mammalian cells via co-cultivation. The incorporated chloroplasts retained their thylakoid structure in intracellular vesicles and were maintained in the cytoplasm, surrounded by the mitochondria near the nucleus. Moreover, the incorporated chloroplasts maintained electron transport activity of photosystem II in cultured mammalian cells for at least 2 days after the incorporation. Our top-down synthetic biology-based approach may serve as a foundation for creating artificially photosynthetic animal cells. 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