https://www.biorxiv.org/content/10.1101/2023.06.27.546656v1 Skip to main content bioRxiv * Home * About * Submit * ALERTS / RSS Search for this keyword [ ] [Search] Advanced Search New Results Neuronal wiring diagram of an adult brain View ORCID ProfileSven Dorkenwald, View ORCID ProfileArie Matsliah, View ORCID ProfileAmy R Sterling, View ORCID ProfilePhilipp Schlegel, View ORCID ProfileSzi-chieh Yu, View ORCID ProfileClaire E. McKellar, View ORCID ProfileAlbert Lin, View ORCID ProfileMarta Costa, View ORCID ProfileKatharina Eichler, View ORCID ProfileYijie Yin, View ORCID ProfileWill Silversmith, View ORCID ProfileCasey Schneider-Mizell, Chris S Jordan, View ORCID ProfileDerrick Brittain, Akhilesh Halageri, Kai Kuehner, Oluwaseun Ogedengbe, View ORCID ProfileRyan Morey, Jay Gager, View ORCID ProfileKrzysztof Kruk, View ORCID ProfileEric Perlman, View ORCID ProfileRunzhe Yang, View ORCID ProfileDavid Deutsch, Doug Bland, Marissa Sorek, Ran Lu, View ORCID ProfileThomas Macrina, Kisuk Lee, View ORCID ProfileJ. Alexander Bae, Shang Mu, Barak Nehoran, View ORCID ProfileEric Mitchell, Sergiy Popovych, View ORCID ProfileJingpeng Wu, Zhen Jia, Manuel Castro, View ORCID ProfileNico Kemnitz, Dodam Ih, View ORCID ProfileAlexander Shakeel Bates, View ORCID ProfileNils Eckstein, View ORCID ProfileJan Funke, View ORCID ProfileForrest Collman, View ORCID ProfileDavi D Bock, View ORCID ProfileGregory S X E Jefferis, View ORCID ProfileH. Sebastian Seung, View ORCID ProfileMala Murthy, the FlyWire Consortium doi: https://doi.org/10.1101/2023.06.27.546656 Sven Dorkenwald 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Sven Dorkenwald Arie Matsliah 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Arie Matsliah Amy R Sterling 3 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Eyewire, Boston, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Amy R Sterling Philipp Schlegel 4 Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK; Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Philipp Schlegel Szi-chieh Yu 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Szi-chieh Yu Claire E. McKellar 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Claire E. McKellar Albert Lin 5 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Center for the Physics of Biological Function, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Albert Lin Marta Costa 6 Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Marta Costa Katharina Eichler 6 Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Katharina Eichler Yijie Yin 6 Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Yijie Yin Will Silversmith 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Will Silversmith Casey Schneider-Mizell 7 Allen Institute for Brain Science, Seattle, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Casey Schneider-Mizell Chris S Jordan 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Derrick Brittain 7 Allen Institute for Brain Science, Seattle, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Derrick Brittain Akhilesh Halageri 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Kai Kuehner 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Oluwaseun Ogedengbe 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Ryan Morey 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Ryan Morey Jay Gager 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Krzysztof Kruk 8 Eyewire, Boston, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Krzysztof Kruk Eric Perlman 9 Yikes LLC, Baltimore, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Eric Perlman Runzhe Yang 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Runzhe Yang David Deutsch 10 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Department of Neurobiology, University of Haifa, Haifa, Israel; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for David Deutsch Doug Bland 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Marissa Sorek 3 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Eyewire, Boston, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Ran Lu 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Thomas Macrina 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Thomas Macrina Kisuk Lee 11 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Brain & Cognitive Sciences Department, Massachusetts Institute of Technology, Cambridge, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site J. Alexander Bae 12 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Electrical and Computer Engineering Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for J. Alexander Bae Shang Mu 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Barak Nehoran 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Eric Mitchell 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Eric Mitchell Sergiy Popovych 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Jingpeng Wu 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Jingpeng Wu Zhen Jia 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Manuel Castro 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Nico Kemnitz 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Nico Kemnitz Dodam Ih 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site Alexander Shakeel Bates 13 Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK; Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; Centre for Neural Circuits and Behaviour, The University of Oxford, Oxford, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Alexander Shakeel Bates Nils Eckstein 14 Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Nils Eckstein Jan Funke 14 Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Jan Funke Forrest Collman 7 Allen Institute for Brain Science, Seattle, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Forrest Collman Davi D Bock 15 Department of Neurological Sciences, University of Vermont, Burlington, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Davi D Bock Gregory S X E Jefferis 4 Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK; Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, UK; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Gregory S X E Jefferis H. Sebastian Seung 1 Princeton Neuroscience Institute, Princeton University, Princeton, USA; Computer Science Department, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for H. Sebastian Seung Mala Murthy 2 Princeton Neuroscience Institute, Princeton University, Princeton, USA; * Find this author on Google Scholar * Find this author on PubMed * Search for this author on this site * ORCID record for Mala Murthy * For correspondence: mmurthy@princeton.edu 16 - * Abstract * Info/History * Metrics * Data/Code * Preview PDF Loading Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains to reconstruct local connectivity maps that are highly informative, yet inadequate for understanding brain function more globally. Here, we present the first neuronal wiring diagram of a whole adult brain, containing 5x10 ^7 chemical synapses between ~130,000 neurons reconstructed from a female Drosophila melanogaster. The resource also incorporates annotations of cell classes and types, nerves, hemilineages, and predictions of neurotransmitter identities. Data products are available by download, programmatic access, and interactive browsing and made interoperable with other fly data resources. We show how to derive a projectome, a map of projections between regions, from the connectome. We demonstrate the tracing of synaptic pathways and the analysis of information flow from inputs (sensory and ascending neurons) to outputs (motor, endocrine, and descending neurons), across both hemispheres, and between the central brain and the optic lobes. Tracing from a subset of photoreceptors all the way to descending motor pathways illustrates how structure can uncover putative circuit mechanisms underlying sensorimotor behaviors. The technologies and open ecosystem of the FlyWire Consortium set the stage for future large-scale connectome projects in other species. Competing Interest Statement T. Macrina, K. Lee, S. Popovych, D. Ih, N. Kemnitz, and H. S. Seung declare financial interests in Zetta AI. Footnotes * https://codex.flywire.ai/ Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license. View the discussion thread. Back to top PreviousNext Posted June 30, 2023. Download PDF Data/Code Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. 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McKellar, Albert Lin, Marta Costa, Katharina Eichler, Yijie Yin, Will Silversmith, Casey Schneider-Mizell, Chris S Jordan, Derrick Brittain, Akhilesh Halageri, Kai Kuehner, Oluwaseun Ogedengbe, Ryan Morey, Jay Gager, Krzysztof Kruk, Eric Perlman, Runzhe Yang, David Deutsch, Doug Bland, Marissa Sorek, Ran Lu, Thomas Macrina, Kisuk Lee, J. Alexander Bae, Shang Mu, Barak Nehoran, Eric Mitchell, Sergiy Popovych, Jingpeng Wu, Zhen Jia, Manuel Castro, Nico Kemnitz, Dodam Ih, Alexander Shakeel Bates, Nils Eckstein, Jan Funke, Forrest Collman, Davi D Bock, Gregory S X E Jefferis, H. Sebastian Seung, Mala Murthy, the FlyWire Consortium bioRxiv 2023.06.27.546656; doi: https://doi.org/10.1101/ 2023.06.27.546656 Share This Article: [https://www.biorxiv.org/content/10.1101/2023.06.27] Copy Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo Citation Tools Neuronal wiring diagram of an adult brain Sven Dorkenwald, Arie Matsliah, Amy R Sterling, Philipp Schlegel, Szi-chieh Yu, Claire E. McKellar, Albert Lin, Marta Costa, Katharina Eichler, Yijie Yin, Will Silversmith, Casey Schneider-Mizell, Chris S Jordan, Derrick Brittain, Akhilesh Halageri, Kai Kuehner, Oluwaseun Ogedengbe, Ryan Morey, Jay Gager, Krzysztof Kruk, Eric Perlman, Runzhe Yang, David Deutsch, Doug Bland, Marissa Sorek, Ran Lu, Thomas Macrina, Kisuk Lee, J. Alexander Bae, Shang Mu, Barak Nehoran, Eric Mitchell, Sergiy Popovych, Jingpeng Wu, Zhen Jia, Manuel Castro, Nico Kemnitz, Dodam Ih, Alexander Shakeel Bates, Nils Eckstein, Jan Funke, Forrest Collman, Davi D Bock, Gregory S X E Jefferis, H. Sebastian Seung, Mala Murthy, the FlyWire Consortium bioRxiv 2023.06.27.546656; doi: https://doi.org/10.1101/ 2023.06.27.546656 Citation Manager Formats * BibTeX * Bookends * EasyBib * EndNote (tagged) * EndNote 8 (xml) * Medlars * Mendeley * Papers * RefWorks Tagged * Ref Manager * RIS * Zotero * Tweet Widget * Facebook Like * Google Plus One Subject Areas All Articles * Animal Behavior and Cognition (4454) * Biochemistry (9783) * Bioengineering (7231) * Bioinformatics (25219) * Biophysics (12825) * Cancer Biology (10123) * Cell Biology (14614) * Clinical Trials (138) * Developmental Biology (8111) * Ecology (12287) * Epidemiology (2067) * Evolutionary Biology (16173) * Genetics (11058) * Genomics (14926) * Immunology (10029) * Microbiology (24036) * Molecular Biology (9650) * Neuroscience (51686) * Paleontology (373) * Pathology (1575) * Pharmacology and Toxicology (2729) * Physiology (4100) * Plant Biology (8802) * Scientific Communication and Education (1526) * Synthetic Biology (2439) * Systems Biology (6523) * Zoology (1369)