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Advertisement Advertisement Nature Communications * View all journals * Search * My Account Login * Explore content * About the journal * Publish with us * Sign up for alerts * RSS feed 1. nature 2. nature communications 3. articles 4. article Identifying the wide diversity of extraterrestrial purine and pyrimidine nucleobases in carbonaceous meteorites Download PDF Download PDF * Article * Open Access * Published: 26 April 2022 Identifying the wide diversity of extraterrestrial purine and pyrimidine nucleobases in carbonaceous meteorites * Yasuhiro Oba ORCID: orcid.org/0000-0002-6852-3604^1, * Yoshinori Takano ORCID: orcid.org/0000-0003-1151-144X^2, * Yoshihiro Furukawa ORCID: orcid.org/0000-0001-7241-530X^3, * Toshiki Koga^2, * Daniel P. Glavin ORCID: orcid.org/0000-0001-7779-7765^4, * Jason P. Dworkin ORCID: orcid.org/0000-0002-3961-8997^4 & * Hiroshi Naraoka ORCID: orcid.org/0000-0002-2373-8759^5 Nature Communications volume 13, Article number: 2008 (2022) Cite this article * 29k Accesses * 2019 Altmetric * Metrics details Subjects * Astrobiology * Meteoritics Abstract The lack of pyrimidine diversity in meteorites remains a mystery since prebiotic chemical models and laboratory experiments have predicted that these compounds can also be produced from chemical precursors found in meteorites. Here we report the detection of nucleobases in three carbonaceous meteorites using state-of-the-art analytical techniques optimized for small-scale quantification of nucleobases down to the range of parts per trillion (ppt). In addition to previously detected purine nucleobases in meteorites such as guanine and adenine, we identify various pyrimidine nucleobases such as cytosine, uracil, and thymine, and their structural isomers such as isocytosine, imidazole-4-carboxylic acid, and 6-methyluracil, respectively. Given the similarity in the molecular distribution of pyrimidines in meteorites and those in photon-processed interstellar ice analogues, some of these derivatives could have been generated by photochemical reactions prevailing in the interstellar medium and later incorporated into asteroids during solar system formation. This study demonstrates that a diversity of meteoritic nucleobases could serve as building blocks of DNA and RNA on the early Earth. Introduction Recent sample return missions from the asteroids Ryugu (C-type) and Bennu (B-type) led by the Japan Aerospace Exploration Agency (JAXA) and the National Aeronautics and Space Administration (NASA), respectively, will provide us with important insights into the evolution of extraterrestrial organic molecules, and potential clues regarding the origins of life on Earth through chemical analyses of pristine extraterrestrial materials that have not been significantly compromised by terrestrial contamination. The scientific justification for choosing C- and B-type asteroids for sample return missions is that these carbon-rich asteroids are plausible parent bodies of carbonaceous chondrites^1,2, in which diverse suites of primordial organic molecules of astrochemical interest, including amino acids and sugars, have been detected^3,4,5,6,7. Moreover, a recent analysis of meteorite extracts using ultra-high-resolution mass spectrometry revealed the presence of several hundreds of thousands of soluble organic species in the Murchison meteorite^8,9, 10, strongly suggesting wide chemical diversity resulting from molecular evolution in each individual thermal history of the parent body. Nucleobases, one of the structural components of nucleic acids, have also been identified in carbonaceous chondrites (ref. ^11 and references therein). There are two types of nucleobases present in DNA and RNA: pyrimidine nucleobases that consist of a single six-membered nitrogen (N) heterocyclic ring whose N atoms are always in the one and three positions and include cytosine (C[4]H[5]N[3]O), uracil (C[4]H[4]N[2]O[2]), and thymine (C[5]H[6]N[2]O[2]), and purine nucleobases that consist of six-membered and five-membered two ring N -heterocyclic structures whose N atoms are always in the 1, 3, 7, and 9 positions, including adenine (C[5]H[5]N[5]) and guanine (C[5]H[5]N [5]O). A diverse suite of exogenous meteoritic organics, including nucleobases, may have been delivered to the early Earth during the late heavy bombardment period (~4.0-3.8 billion years ago);^12,13 therefore, the influx of such organics is considered to have played an important role in the chemical evolution of the Earth's primordial stage. A pioneering study tracing nucleobases in carbonaceous meteorites used UV-visible spectroscopy and thin-layer chromatography to detect nucleobases extracted from 16 g of the Orgueil meteorite in the early 1960s^14, which reported the presence of N-heterocyclic molecules, including adenine and guanine, with concentrations ranging from 11-20 mg/g-meteorite or parts per million (ppm). Further detailed analyses have been conducted using more advanced methods to investigate nucleobases from other types of carbonaceous meteorites^ 15,16,17,18,19. However, the number of nucleobases indigenous to meteorites detected to date is at most eight (seven purine bases and one pyrimidine base)^11, which is much more limited compared to the 96 different amino acids that have been identified by name in the Murchison meteorite^7. In contrast to the limited number of nucleobases identified in meteorites, various nucleobases and related nitrogen heterocyclic molecules have been synthesized by laboratory-based simulation experiments, such as conditions simulating interstellar molecular clouds (~10 K) and meteorite parent bodies^20,21,22,23,24,25, suggesting that these classes of organic compounds are ubiquitously present in extraterrestrial environments both inside and outside the solar system. Recent advances in analytical techniques and instruments have enabled the detection and quantification of nucleobases in mixtures of complex organic molecules such as meteorite extracts and organic residues in interstellar ice analogs, even at parts per billion levels (ppb)^19,25. Callahan et al.^19 analyzed extracts from 12 carbonaceous meteorites and identified six purine bases, including guanine at a concentration of 20 ppb, which is three orders of magnitude lower than that reported by Hayatsu^14. More recently, Oba et al.^25 reported a further improvement of very small-scale analyses (<100 pg per analysis) for purine and pyrimidine nucleobases using an optimized wet-chemical treatment, providing chromatographic baseline resolution for N-containing heterocyclic target molecules. Thus, we expect that the robust method described here can be applied to organic extracts from carbonaceous meteorites and returned samples using liquid chromatography coupled with high-resolution mass spectrometry^25,26. In the present study, we analyze nucleobases extracted from the Murchison, Murray, and Tagish Lake meteorites using high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (HPLC/ESI-HRMS) to examine the molecular profiles and determine the abundance of N-containing heterocyclic molecules (Supplementary Fig. 1) in these meteorites. In addition, we analyze aqueous extracts of different Murchison meteorite specimens and soil from the Murchison meteorite fall site to determine variations in nucleobase concentrations within the same meteorite and potential sources of terrestrial contaminants. Results and discussion Purine nucleobases and related molecules in the Murchison extracts We detected several purine nucleobases and their analogs in both Murchison meteorite extracts (Fig. 1, Supplementary Fig. S2, and Table 1) based on their chromatographic retention time, accurate mass measurements of their parent masses, and mass fragmentation patterns in the MS/MS measurements (Fig. 1b). Guanine is typically the most abundant purine nucleobase (hereafter referred to as purine molecules, unless otherwise denoted) in all meteorites (except for Tagish Lake), followed by adenine, xanthine, and hypoxanthine as the second most abundant group, with purine, isoguanine, and 2,6-diaminopurine as the least abundant group. The total concentrations of purine molecules were 152 and 11 ppb in the aqueous extracts of two Murchison meteorite specimens (one obtained from the University of Chicago and the other from a meteorite trading company. Hereafter, each specimen is denoted as Murchison #1 and Murchison #2, respectively). Although the concentrations in the Murchison #1 extract and those reported by Callahan et al.^19 are similar, those in the Murchison #2 extract were generally one order of magnitude lower. Because the analytical conditions used for the two Murchison specimens in the present study were essentially identical, we can infer that the observed difference was due to sample heterogeneity within the Murchison meteorite (e.g., meteoritic amino acid profiles of Murchison)^8,27. Significant heterogeneity in amino acid abundance was previously reported in different lithologies of the Tagish Lake meteorite, where total amino acid concentrations ranged from 40 ppb in the most aqueously altered samples up to 5400 ppb in the least altered lithologies^28. The weighted average of the concentrations of purine molecules in the two Murchison specimens is shown in Table 1 and has a similar order of magnitude as that reported by Callahan et al.^19. Note that there is a striking difference between the extraction method used in the current study and that used by Callahan et al.^19, who extracted meteorites in 95% formic acid at 100 degC for over 24 h. If such severe extraction conditions are more suitable for the extraction of nucleobases from meteorites, it is likely that the actual concentrations of nucleobases in our meteorite samples are higher than those reported here. Nevertheless, in the present study, we used a less harsh extraction method to prevent the undesirable decomposition of fragile molecules such as sugars^6 and hexamethylenetetramine^26 present in the meteorite specimens. Consequently, the weighted average concentrations in our Murchison extracts were lower than those reported previously. We concluded that the detection of purine (C[5]H[4]N[4]) in the meteorite extracts strongly supported the indigenous nature of the detected purine molecules in the Murchison meteorites^19. Fig. 1: Detection of purine nucleobases. figure 1 Mass chromatograms at m/z = 152.0567 within a 3 ppm exact mass window at the monoisotopic mass for a the guanine (0.21 ng) standard reagent (STD) and b Murchison #1 extract were measured using an InertSustain PFP column. Additionally, isoguanine, a structural isomer of guanine, was detected in the Murchison #1 extract. Mass fragmentation patterns of c guanine STD and d guanine were detected in the Murchison #1 extract measured by MS/MS experiments. Note: "exact mass", representing a theoretical value obtained by calculation, is different from "accurate mass", which is a measured value with a high-resolution mass spectrometer. Bold italic numbers in panel a represent the numbering of atoms in the chemical structures of purine nucleobases. Full size image Table 1 Variations in the concentration (in ppb) of purine nucleobases and their analogs in the meteorite extracts and Murchison soil. Full size table Pyrimidine nucleobases in the Murchison extracts We detected a wide variety of pyrimidine nucleobases and their structural isomers from both Murchison extracts (Fig. 2, Supplementary Figs. 3, 4, and Table 2), most of which had not been previously detected in meteorites. Uracil is the only pyrimidine nucleobase previously quantified in the Murchison meteorite (30 ppb in an H[2]O extract)^29. Although the abundance was not quantified, uracil was also detected in the formic acid extracts of the Murchison meteorite, and the stable carbon isotopic composition (d^13C) was measured to be +44.5%0 (vs. VPDB standard)^18. The uracil concentrations in the Murchison #1 and #2 meteorites were 15 and 1 ppb, respectively, showing that uracil is distributed heterogeneously in the Murchison meteorite. The weighted average of the uracil concentration (7 ppb) was approximately four times lower than that reported in the previous study^29, likely due to Murchison sample heterogeneity. In addition, the uracil concentration in the previous study could have been overestimated because of the co-elution of other molecules, such as carboxylic acids. As shown in Fig. 2a, in addition to uracil, we detected the structural isomers 2-imidazolecarboxylic acid and 4-imidazolecarboxylic acid in the same extract, and their concentrations exceeded that of uracil (Table 2). If the peak separation between uracil and its isomers is inadequate, as in the case shown in Supplementary Fig. 5, the uracil concentration would be overestimated. Hence, further confirmation using an appropriate LC condition (Fig. 2a) and MS/MS measurements (Fig. 2b and Supplementary Fig. 4) are required to identify a specific molecule in a mixture of complex molecules containing structural isomers. Fig. 2: Detection of pyrimidine nucleobases. figure 2 Mass chromatograms at m/z = 113.0346 within a 3 ppm exact mass window at each monoisotopic mass for a the uracil (0.46 ng) standard reagent (STD) and b Murchison #1 extract measured using a Hypercarb column. In addition to uracil, 4-imidazolecarboxylic acid and 2-imidazolecarboxylic acid were detected as the dominant structural isomers in the Murchison #1 extract. Mass fragmentation patterns of c uracil STD and d uracil were detected in the Murchison #1 extract measured by MS/MS experiments. Mass peaks with a star (*) represent background signals in the fragmentation spectra present in both the meteorite and reference STD spectra. Bold italic numbers in panel a represent the numbering of atoms in the chemical structures of pyrimidine nucleobases. Full size image Table 2 Variations in the concentration (in ppb) of pyrimidine nucleobases and their related analogs in meteorite extracts and the Murchison soil. Full size table Other pyrimidine nucleobases, such as cytosine and thymine, as well as their analogs containing a pyrimidine ring, were identified by their chromatographic retention times, accurate mass measurements of their parent masses, and mass fragmentation patterns in the MS/MS measurements (Supplementary Table 1), with concentrations ranging from 0.02 to 6 ppb (Table 2). Methylated pyrimidine nucleobases (except thymine, a methylated form of uracil), such as 5-methylcytosine, 1-methyluracil, and 6-methyluracil, are less common in biological systems than major bases such as cytosine and uracil^30 ,31,32. The observed similar concentrations of methylated and non-methylated pyrimidine nucleobases strongly suggest that they are abiological and hence extraterrestrial in origin. Though the presence of the relatively unstable cytosine^33 is unexpected and would typically be attributed to contamination, their presence in abundances similar to isomers and methylated analogs reinforces the abiological origin of cytosine. The reason for the preservation of cytosine is unclear, perhaps it was produced at a steady state in the meteorite parent bodies and protected from deamination due to rapid drying and/or low temperatures once the hydrothermal phase on the asteroid had stopped. In addition, some coexisting species on the asteroid could have a role for the preservation of cytosine, which will be investigated in our future study. The total concentrations of the detected pyrimidine nucleobases and their analogs (hereafter referred to as pyrimidine molecules, unless otherwise denoted) were 37 and 15 ppb in Murchison #1 and Murchison # 2, respectively, and the weighted average was 24 ppb, which is ~0.1% of the total amino acid concentration in the Murchison meteorite^3. The much lower concentrations of pyrimidine and purine molecules compared to meteoritic amino acids suggest that nucleobases are less suitable for formation in extraterrestrial environments, including meteorite parent bodies. Pyrimidine (C[4]H[4]N[2]) was not identified in the two Murchison extracts, which is consistent with organic residues in UV-processed interstellar ice analogs^25. The absence of pyrimidine in meteorite extracts suggests that the addition of side chains such as methyl (-CH[3]) and amino (-NH[2]) groups to pyrimidine may not be the dominant pathway to the formation of the detected pyrimidine nucleobases. Assessment of terrestrial contamination of nucleobases in the Murchison extract The analysis of extracts from the procedural blank that consisted of prebaked quartz did not yield any detectable nucleobases, indicating no terrestrial nucleobase contamination during the analytical procedures. However, we detected pyrimidine and purine nucleobases from the Murchison soil extract at concentrations ranging from 1 to 141 ppb (Tables 1 and 2), which were generally higher than those in the Murchison extracts (Tables 1 and 2). Callahan et al.^19 also detected purine and pyrimidine nucleobases in the Murchison soil formic acid extract at concentrations ranging from 7 to 1380 ppb, with cytosine being the most abundant. In contrast, except for DNA/ RNA nucleobases, the Murchison soil showed less molecular diversity than the formic acid extract of the Murchison meteorite. Based on significant differences in the distributions of nucleobases and their analogs between the Murchison meteorite and soil extracts, including purine, 2,6-diaminopurine, and 6,8-diaminopurine, which were detected in the meteorite extract but not in the soil, they concluded that purines found in the Murchison meteorite are extraterrestrial in origin. We used two Murchison meteorite specimens: a 2 g specimen from the University of Chicago (Murchison #1) and a 2.7 g specimen from a meteorite trading company (Murchison #2). If terrestrial contamination dominates the meteoritic nucleobase distribution in the present study, it indicates that the meteorite size and nucleobase concentration are positively correlated. However, the actual distributions were opposite, with the concentrations of nucleobases in Murchison #1 generally being much higher than those in Murchison # 2 (Tables 1 and 2); this strongly suggests that the nucleobases in the soil are not inherited by the meteorite and hence the detected nucleobases are all extraterrestrial in origin. In addition, other purine and pyrimidine molecules, such as isoguanine, 5-methylisocytosine, and purine, were not detected in the soil extracts, supporting the assumption described above. Note that alanine extracted from Murchison #1 was racemic^34, which strongly suggests that the detected alanine is extraterrestrial in origin with no evidence of significant biological contamination of the meteorite sample. This would also be the case for nucleobases. Moreover, in general, the higher concentration of dihydrouracil compared to nucleobases cannot be explained by terrestrial biology^35. Hence, we conclude that the observed chemical diversity of the purine and pyrimidine molecules and their isomers are indigenous and suggests an extraterrestrial origin, consistent with the detection of racemic amino acids and primordial sugars from the same extracts^6,7. Purine and pyrimidine molecules and their isomers in the Tagish Lake and Murray extracts Various purine and pyrimidine molecules were also identified in the Tagish Lake and Murray extracts (Tables 1 and 2). Guanine was the most abundant in the Murray extract (25 ppb), as is the case for the two Murchison extracts, whereas, for the Tagish Lake extract, adenine was the most abundant (6 ppb) purine molecule and 6-methyluracil (17 ppb) is the most abundant pyrimidine molecule. The Tagish Lake extract generally shows less diversity of purine and pyrimidine molecules compared with the Murchison and Murray extracts, and their absolute concentrations are generally lower than those of the other meteorite extracts. The observed trend in molecular abundance and diversity is consistent with that observed for amino acids in these three meteorites^28, suggesting that the Tagish Lake and the other two meteorites may have experienced distinct processes on their parent bodies. The total concentrations of detected purine molecules in the Tagish Lake and Murray extracts were 9 and 33 ppb, respectively, and those of pyrimidine molecules were 37 and 13 ppb, respectively. Note that there is a prominent difference in the (total pyrimidine molecules)/(total purine molecules) ratio between the Tagish Lake and the other two meteorites: for the former, pyrimidine molecules dominate over purine molecules, whereas the opposite is true for the latter. This observed difference may reflect different formation pathways, as discussed later. Important nitrogen heterocyclic molecules in the meteorite extracts Imidazole is an interesting molecule in terms of a primitive catalyst for molecular syntheses^36, which might be a potential precursor of purine formation^37. Due to its potential importance, it has been a target of multiple astrochemical^25,37 and astronomical studies^38. Here, we identified not only imidazole itself but also its related analogs, some of which are the structural isomers of pyrimidine nucleobases such as cytosine, uracil, and thymine at typically higher concentrations than the corresponding pyrimidine bases (Fig. 2 and Supplementary Fig. 3). For example, 4-imidazolecarboxylic acid and 1-methyl-1H-imidazole-5-carboxylic acid, which are structural isomers of uracil and thymine, respectively, are 1-2 orders of magnitude more abundant than the corresponding pyrimidine nucleobases (Table 2). This strongly suggests the presence of preferential routes for the formation of imidazole-containing molecules over pyrimidine-containing molecules in extraterrestrial environments. The reactivity of imidazole and the contribution of electron-conjugated systems are assumed to induce progressive cyclization and variation in purine derivatives. Indeed, imidazole and its alkylated homologs are much more abundant than pyrimidine nucleobases (Table 3), supporting our hypothesis. We estimated that the progression of the N-heterocyclic reaction would correlate with the thermal history of the parent body. Table 3 Variations in the concentrations (in ppb) of imidazole and its alkylated analogs. Full size table We also confirmed the presence of alkylated homologs of imidazole using the same procedure described above (Supplementary Fig. 6). Larger alkylated imidazole homologs were observed in the mass chromatograms using a retention index of systematic methylene (-CH[2] -) elongation (Supplementary Fig. 7). These results are qualitatively consistent with the tentative detection of alkylated imidazole homologs (i.e., imidazole with 95.0 +- 3.5%, n = 3)^25. All glassware was cleaned by heating in air at 450 degC for 3 h prior to being used for sample processing and molecular analysis. Synthesis of photochemical products We analyzed the organic residues produced by the photolysis of ice mixtures containing H[2]O, CO, NH[3], and CH[3]OH under astrophysically relevant conditions at 10 K. Details of the preparation scheme have been reported in a previous study^25. To summarize, the ice mixture was photolyzed on an aluminum substrate at 10 K in an experimental setup (SAMRAI) equipped with a stainless-steel chamber, two deuterium lamps, a quadrupole mass spectrometer, a turbo molecular pump, and a helium cryostat. After photolysis, the sample was warmed to room temperature and the formed organic residues were extracted with ~0.5 mL of a mixture of H[2]O/CH [3]OH (1/1, v/v). The extracted samples were treated using the same procedure as described above. Chromatographic separation and time-based identification of nucleobases by HPLC Some heterocyclic compounds have the same chemical composition and exact mass number (e.g., cytosine and isocytosine; guanine and isoguanine); therefore, chromatographic separation is an important process for the optimization of online mass spectrometry. The purified fraction of each sample was introduced into an HPLC/ESI-HRMS instrument with a mass resolution of 140,000 at a mass-to-charge ratio (m/z) of 200. The online system comprised an UltiMate 3000 and Q-Exactive Plus (Thermo Fischer Scientific) equipped with a reversed-phase separation column (InertSustain PFP, 2.1 x 250 mm, particle size 3 mm, GL Science or HyperCarb^TM, 2.1 x 150 mm, particle size 5 mm, Thermo Fischer Scientific) at 40 degC and operated in positive ion mode. Validations of analytical methods were also performed using a 1290 Infinity II coupled with a 6230 time-of-flight mass spectrometer (Agilent Technologies). The eluent program for the HPLC setup with the PFP column was as follows: at t = 0 to 5 min, solvent A (water), solvent B (acetonitrile + 0.1% formic acid) = 90:10, followed by a linear gradient of A:B = 50:50 at 20 min and maintained at this ratio for 25 min. The flow rate was 0.1 mL/min. The eluent program for the HyperCarb^TM column was as follows: solvent A (water + 0.1% formic acid) and solvent B (acetonitrile + 0.1% formic acid) = 100:0 at t = 0 min, followed by a linear gradient of A:B = 75:25 at 20 min and maintained at this ratio for 15 min. We used an improved gradient program to maintain ionization efficiency because the addition of 0.1% formic acid (as a proton donor) to solvents A and B created an inverse elution and ensured the stability of the electron spray ionization efficiency. The flow rate was constantly 0.2 mL/min. The PFP column was especially suitable for the chromatographic separation of purine nucleobases, but was inappropriate for the analysis of pyrimidine nucleobases because co-eluting structural isomers prevented some target molecule identification and quantification. On the other hand, the use of HyperCarb^TM has a strong advantage for the separation of pyrimidine nucleobases because of the appropriate affinities between nitrogen-containing molecules and the stationary phase. Molecules that were not detected in positive ion mode, such as 5-amino-4-imidazolecarboxamide (Supplementary Fig. 12) and 5-aminoimidazole-4-carbonitrile (Supplementary Fig. 13), were analyzed in negative ion mode using the same instrument but with a HILICpak VG-50 column (2.1 x 150 mm, particle size 5 mm, Shodex) at 40 degC. The elution program was as follows: solvent A (H[2]O + 0.5% ammonia) and solvent B (acetonitrile) = 20:80 at t = 0-5 min, followed by a linear gradient of A:B = 90:10 at 35 min and maintained at this ratio for 10 min. The flow rate was 0.2 mL/min. High-purity grade water and acetonitrile (LC/MS grade from Fujifilm Wako Chemical, Ltd.) were used to minimize analytical noise and background signals in the LC and Orbitrap MS. Exact mass identification of nucleobases by HRMS The mass spectra were recorded in positive or negative ESI mode with an m/z range of 50-400 and a spray voltage of 3.5 kV. We confirmed the MS response linearity over a dynamic range of substrate concentrations on the order of ppt, ppb, and ppm scales^25,26. The exact mass was obtained with a general mass accuracy of 3 ppm, defined as [(measured m/z) - (calculated m/z)]/(calculated m/z) x 10^ 6 (ppm). The separated compound solution was heated at 300 degC for desolvation using a HESI-II (Thermo Fischer Scientific) or Agilent Jet Stream (Agilent Technologies). The capillary temperature of the ion transfer was 300 degC. The injected samples were vaporized at 300 degC. MS/MS experiments were also performed using a hybrid quadrupole-Orbitrap mass spectrometer (Q-Exactive Plus, Thermo Fisher Scientific) with HPLC and ionization conditions identical to those used for the full-scan analyses. The extracted positive ion m/z (for example, for thymine, 127.05 +- 0.2) was reacted with high-energy collision N[2] gas to produce fragmented ions, and the mass range of m/z 50-160 was monitored using an Orbitrap MS with a mass resolution of ~140,000. MS/MS measurements were not performed for some nucleobases because of their low concentrations in the extracts. Standard reagents for the target molecules (Supplementary Fig. 1) were purchased from Combi-Blocks Inc., Toronto Research Chemicals, Fujifilm Wako Chemical, Ltd., Tokyo Chemical Industry, Ltd., Sigma-Aldrich Ltd., and BLD Pharmatech, Ltd., and used without further purification. Procedural blank test The analytical blank test was performed using 2 g of combusted quartz subjected to the same extraction procedure described above to evaluate possible contamination by nucleobases during sample preparation. 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Matthews' research group at the University of Chicago for providing the Murchison meteorite and Prof. Reid R. Keays (Univ. Melbourne) for collecting and providing the Murchison soil samples. We also thank Mr. Tomoya Ishida (Kyushu Univ.) for the discussion of the wet-chemical procedure. This work was partly supported by JSPS KAKENHI Grant Numbers JP21H04501 and JP21H05414 (to Y.O.), JP20H02019 and 21KK0062 (to Y.T.), 21J00504 (to T.K), JP20H00202 and JP20H05846 (to H.N.), NASA Astrobiology Institute through award 13-13NAI7-0032 to the Goddard Center for Astrobiology (GCA), NASA's Planetary Science Division Internal Scientist Funding Program through the Fundamental Laboratory Research (FLaRe) work package at NASA Goddard Space Flight Center, and a grant from the Simons Foundation (SCOL award 302497 to J.P.D.). This study was conducted in accordance with the Joint Research Promotion Project at the Institute of Low-Temperature Science, Hokkaido University (21G008 to Y.O., Y.T., and H.N.). Author information Affiliations 1. Institute of Low Temperature Science (ILTS), Hokkaido University, N19W8, Kita-ku, Sapporo, Hokkaido, 060-0189, Japan Yasuhiro Oba 2. Biogeochemistry Research Center (BGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa, 237-0061, Japan Yoshinori Takano & Toshiki Koga 3. Department of Earth Science, Tohoku University, 6-3 Aza-aoba, Aramaki, Aoba-ku, Sendai, 980-8578, Japan Yoshihiro Furukawa 4. Solar System Exploration Division, National Aeronautics and Space Administration (NASA), Goddard Space Flight Center (GSFC), Greenbelt, MD, 20771, USA Daniel P. Glavin & Jason P. Dworkin 5. Department of Earth and Planetary Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Fukuoka, 819-0395, Japan Hiroshi Naraoka Authors 1. Yasuhiro Oba View author publications You can also search for this author in PubMed Google Scholar 2. Yoshinori Takano View author publications You can also search for this author in PubMed Google Scholar 3. Yoshihiro Furukawa View author publications You can also search for this author in PubMed Google Scholar 4. Toshiki Koga View author publications You can also search for this author in PubMed Google Scholar 5. Daniel P. Glavin View author publications You can also search for this author in PubMed Google Scholar 6. Jason P. Dworkin View author publications You can also search for this author in PubMed Google Scholar 7. Hiroshi Naraoka View author publications You can also search for this author in PubMed Google Scholar Contributions Y.O. and Y.T. designed this project, outlined the development with H.N. For sample preparation, D.P.G. and J.P.D. performed the pretreatment and quality assessment of the Murchison sample from Univ. Chicago. Y.T. and Y. F. extracted indigenous nitrogen compounds from meteorites and purified them before LC/ESI-HRMS analysis. Y.O., Y.T., Y.F., and D.P.G. conducted an assessment using a reference soil sample from the Murchison meteorite fall locality in Murchison, Australia. Y.O., T.K., and H.N. analyzed the samples with authentic standards and reference materials. Y.O. prepared the manuscript. All authors commented on the manuscript. Corresponding author Correspondence to Yasuhiro Oba. Ethics declarations Competing interests The authors declare no competing interests. Peer review Peer review information Nature Communications thanks Hikaru Yabuta and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available. Additional information Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Supplementary information Supplementary Information Peer Review File Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and Permissions About this article Verify currency and authenticity via CrossMark Cite this article Oba, Y., Takano, Y., Furukawa, Y. et al. Identifying the wide diversity of extraterrestrial purine and pyrimidine nucleobases in carbonaceous meteorites. Nat Commun 13, 2008 (2022). https://doi.org/ 10.1038/s41467-022-29612-x Download citation * Received: 20 October 2021 * Accepted: 22 March 2022 * Published: 26 April 2022 * DOI: https://doi.org/10.1038/s41467-022-29612-x Share this article Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. 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