CONTENTS.


PART I.
Introduction 11
On Cutting Unprepared Vegetable Tissues 12
On Cutting Unprepared Animal Tissues 13
Preparation of Vegetable Tissues 15
Preparation of Animal Tissues 16
Special Methods of Hardening 19
Cutting Hardened Tissues by Hand 19
Microtome 20
Æther Microtome 24
Section-Knife 24
Imbedding in Paraffine for Microtome 26
Employment of Microtome 29
Staining Agents 31
Carmine Staining 32
Mounting Media 36
Mounting in Glycerine 38
Uses of Freezing Microtome 42
Employment of Freezing Microtome 43
Logwood Staining 47
Absolute Alcohol 50
Clove Oil 51
Canada Balsam 51
Mounting in Balsam 53
Finishing the Slide 55
PART II.
Special Methods 57
Bone 57
Brain 59
Cartilage 60
Coffee Berry 62
Fat 63
Hair 63
Horn, etc. 64
Intestine 64
Liver 65
Lung 65
Muscle 66
Orange-peel 67
Ovary 67
Porcupine Quill 67
Potato 67
Rush 68
Skin 68
Spinal Cord 69
Sponge 71
Stomach 71
Tongue 71
Vegetable Ivory 72
Wood 72
Note A, 75
Note B, 75
Note C, 76
Note D, 76
Note E, 76
Note F, 77
Note G, 77
Note H, 93
Note I, 94
Index