From dgalbraith@rbg.ca Thu Nov 2 04:54:40 2000 Received: from mxu4.u.washington.edu (mxu4.u.washington.edu [140.142.33.8]) by lists.u.washington.edu (8.9.3+UW00.05/8.9.3+UW99.09) with ESMTP id EAA374142 for ; Thu, 2 Nov 2000 04:54:39 -0800 Received: from exchserver.rbg.ca (rbg.ca [207.236.61.195]) by mxu4.u.washington.edu (8.9.3+UW00.02/8.9.3+UW99.09) with ESMTP id EAA19229 for ; Thu, 2 Nov 2000 04:54:39 -0800 Received: by EXCHSERVER with Internet Mail Service (5.5.2650.21) id ; Thu, 2 Nov 2000 08:02:39 -0500 Message-ID: <5619D6434A1AD411859D00E018C2BA8304A456@EXCHSERVER> From: David Galbraith To: "'CONSBIO Conservation Biology List'" Subject: Field preservation of plant tissues Date: Thu, 2 Nov 2000 08:02:37 -0500 MIME-Version: 1.0 X-Mailer: Internet Mail Service (5.5.2650.21) Content-Type: text/plain I would be very grateful to hear from anyone who has a practical protocol for field preservation of plant tissues for later DNA extraction that uses a non-refrigerated, non-toxic field preservation method. I have the CTAB/NaCl buffer method from Steve Rogstad at University of Cincinnati, but unfortunately the CTAB buffer is toxic. Thanks, David A. Galbraith, Ph.D. Coordinator, Botanical Conservation Office Royal Botanical Gardens Hamilton/Burlington, Ontario, Canada Tel: (905) 527-1158, ext. 309 Fax: (905) 577-0375 Email: dgalbraith@rbg.ca URL: http://www.rbg.ca/cbcn .