From owner-drosophila@net.bio.net Mon May 29 23:00:00 1995
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From: Krishnan Balaji <krishnan@sol4>
Newsgroups: bionet.drosophila
Subject: Re: help with food
Date: Tue, 30 May 1995 19:09:59 +0200
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Dear John,
	1. do not keep the media in the cold room for 4 days.  Instead 
pour it in the afternoon and use it immediately.
	2.  Use sucrose and glucose as the sugars.
	3.  Add some yeast granules before putting the flies into the 
vial for egg-laying.
	4. Till you get say 25-30 flies as the progenyof the first 
inoculation, keep the flies in the media for egg-laying upto 5-6 days.
	5. use as many as 25 flies for bottle culture and 5-10 flies for 
vial culture.
That's it.  Also refer the book.
"Drosophila - A practical approach" by D.B. Roberts. IRL press
if you want more details.

Hope you find the sugggestions valuable.

PERMANENT ADDRESS
Balaji Krishnan
Mol. Bio. Unit.
Homi Bhabha Road, TIFR, Colaba
Bombay - 400 005.
INDIA
E-mail: Balajik@tifrvax.tifr.res.in

.