From owner-celegans@net.bio.net Wed Sep 28 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!news.bc.net!news.mic.ucla.edu!steve3.mbi.ucla.edu!rkagan From: Ron Kagan Newsgroups: bionet.celegans Subject: Re: Worm diet Date: 28 Sep 1994 22:05:35 GMT Organization: Dept. of Chemistry and Biochemistry, ULCA Lines: 28 Distribution: world Message-ID: <36cpbf$g6b@news.mic.ucla.edu> References: NNTP-Posting-Host: steve3.mbi.ucla.edu X-UserAgent: Nuntius v1.1.2 X-XXMessage-ID: X-XXDate: Wed, 28 Sep 94 15:08:40 GMT In article Peg MacMorris, macmorri@bio.indiana.edu writes: >We routinely store pellets of frozen E. coli (NA22) and use them to feed >worms in liquid culture. We grow a large fermenter batch of E. coli and >then harvest and freeze the bacteria in weigh boats. The greatest problem >is that the apparatus and procedure we use for harvesting are not designed >for maintaining sterility of the cultures. Therefore, it is easy to >introduce contaminating microbes to worm cultures, although the greater >biomass is E.coli. Again that is not such a problem, especially if the >liquid culture is to be harvested for a large biochemical prep as opposed >to subsequent culturing. Thanks to all those who replied regarding the use of frozen worms. Four people have replied and all agree that frozen E. coli may be used in liquid nematode cultures. Ron Kagan ********************************************************************** "My Kindness is not random, and nothing senseless was ever beautiful." - J. Zabriskie ********************************************************************** Ron Kagan rkagan@ewald.mbi.ucla.edu .