From owner-celegans@net.bio.net Sun Jun 09 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!fu-berlin.de!news.belwue.de!news.uni-hohenheim.de!wpxx02.toxi.uni-wuerzburg.de!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins,bionet.celegans Subject: Re: help with a biochemistry lab Followup-To: bionet.molbio.proteins,bionet.celegans Date: 10 Jun 1996 18:09:31 GMT Organization: CC University of Hohenheim (not responsible for contents) Lines: 19 Message-ID: <4phocr$k88@power5.rz.uni-hohenheim.de> References: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.molbio.proteins:8087 bionet.celegans:932 Bruce Wightman (wightman@mendel.berkeley.edu) wrote: > I was wondering if anyone could provide suggestions for good proteins to > partially purify/purify for an introductory biochemistry lab for > undergraduates. Maybe GST would work. It's a single-step purification from bacteria which can be done easily in two days, yielding milligrams of pure protein from one liter of bacterial culture. If you prepare it at room temperature, yields will probably drop slightly, but who cares. Affinity is assayed photo- metrically with Dichloronitrobenzol plus Glutathione. Just a suggestion, --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ .