From owner-dros@hgmp.mrc.ac.uk Tue Apr 6 18:41:13 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id E60857D20D for ; Tue, 6 Apr 2004 18:41:12 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 729CD7D218 for ; Tue, 6 Apr 2004 18:41:12 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 542EC7D20D; Tue, 6 Apr 2004 18:41:11 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id EAFB57D11F for ; Fri, 2 Apr 2004 20:09:21 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id CE43F7D16E; Fri, 2 Apr 2004 20:09:20 +0100 (BST) To: bionet-drosophila@net.bio.net From: thomaskosh@hotmail.com ("thomas kosh") Newsgroups: bionet.drosophila Subject: mounting in parafin Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: MIME-Version: 1.0 Content-Type: multipart/alternative; boundary="----=_NextPart_000_0005_01C418BC.5753E250" X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CBF6E7D1D4 for ; Fri, 2 Apr 2004 20:09:19 +0100 (BST) X-Received: from hotmail.com (bay8-dav24.bay8.hotmail.com [64.4.26.81]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 204797D11F for ; Fri, 2 Apr 2004 20:09:15 +0100 (BST) X-Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; Fri, 2 Apr 2004 11:09:13 -0800 X-Received: from 147.72.101.2 by bay8-dav24.bay8.hotmail.com with DAV; Fri, 02 Apr 2004 19:09:13 +0000 X-Originating-IP: [147.72.101.2] X-Originating-Email: [thomaskosh@hotmail.com] X-Sender: thomaskosh@hotmail.com X-To: X-Priority: 3 X-MSMail-Priority: Normal X-Mailer: Microsoft Outlook Express 6.00.2800.1158 X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1165 X-OriginalArrivalTime: 02 Apr 2004 19:09:13.0916 (UTC) FILETIME=[FCAF97C0:01C418E5] Date: Tue, 6 Apr 2004 18:41:11 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk This is a multi-part message in MIME format. ------=_NextPart_000_0005_01C418BC.5753E250 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable howdy, i am new to drosophila and am trying (unsuccessfully) to make serial = sections of their brains using a microtome and parafin. does anyone have = a protocol for fixing, dehydrating, clearing, etc. flies? If so could you please e-mail it to me at thomaskosh@hotmail.com thanks a bunch! ------=_NextPart_000_0005_01C418BC.5753E250 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
howdy,
i am new to drosophila and am trying=20 (unsuccessfully) to make serial sections of their brains using a=20 microtome and parafin. does anyone have a protocol for = fixing,=20 dehydrating, clearing, etc. flies?
If so could you please e-mail it to me = at thomaskosh@hotmail.com<= /DIV>
 
thanks a = bunch!
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