From owner-drosophila@net.bio.net Thu Sep 29 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!howland.reston.ans.net!wupost!cerberus-138.wustl.edu!medicine!riesenbe From: riesenbe@medicine (Brad Riesenberger) Newsgroups: bionet.drosophila Subject: Re: SL-2 Cell culture Date: 30 Sep 1994 15:48:45 GMT Organization: Washington University School of Medicine Lines: 31 Message-ID: <36hc0t$6an@cerberus-138.wustl.edu> References: NNTP-Posting-Host: medicine.wustl.edu nwinegar@tuzo.erin (Neil Winegarden) writes: Try calling Sigma Cell Culture tech. service at 800-848-7791. Ask for Dr. Terry Johnson. Regards, Brad Riesenberger riesenbe@medicine.wustl.edu : >I am having a problem with my SL-2 culture and was wondering if anyone >has ever run into similar problems. >I am growing cells in spinner flask cultures, using Shields and Sang's M3 >media and 10% FCS. Originally I had no problem getting the cells up to a >good concentration and then all of a sudden, they stopped growing. They >are not dead (I have done viability assays) yet they remain at a >concentration which is only one third to half what I used to get them to. >I have tried adding more serum, and glutamine to my media. >I'm not sure what else to try. >Any help would be greatly appreciated. >Thank-you, >-- >________________________________ >Neil Winegarden >University Of Toronto >nwinegar@credit.erin.utoronto.ca .