From owner-drosophila@net.bio.net Mon May 22 23:00:00 1995
Path: biosci!ns1.faseb.org!darwin.sura.net!cdc1.cdc.gov!CIDDPD2.EM.CDC.GOV!MQB0
From: MQB0@CIDDPD2.EM.CDC.GOV (Mark Q. Benedict)
Newsgroups: bionet.drosophila
Subject: Blocking microsatellite hybridization in situ
Date: Thu, 18 May 1995 07:56:14 UNDEFINED
Organization: Centers for Disease Control
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Message-ID: <MQB0.2.001022BF@CIDDPD2.EM.CDC.GOV>
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Fellow Users,
We are hybridizing fairly short PCR-amplified microsatellite-containing 
DNAs to polytene chromosomes of the malaria mosquito Anopheles gambiae.  We 
would like to block the secondary hybridization sites with microsatellite 
oligos.  Could you suggest a reliable method for doing so?  (We use 
biotin-labeled DNAs.)

Thanks,
Mark Q. Benedict
Emory U./Centers for Disease Control and Prevention

.