From owner-dros@hgmp.mrc.ac.uk Thu Jun 5 15:30:23 2003 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 869D97D192 for ; Thu, 5 Jun 2003 15:30:23 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E26867D372 for ; Thu, 5 Jun 2003 15:30:22 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 151AA7D192; Thu, 5 Jun 2003 15:30:22 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3647B7D1B4 for ; Mon, 2 Jun 2003 22:44:27 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 85C587D0F3; Mon, 2 Jun 2003 22:44:26 +0100 (BST) To: bionet-drosophila@net.bio.net From: parkerj@uci.edu (Louise Parker) Newsgroups: bionet.drosophila Subject: targeted gene knockout Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <5EF97670-9543-11D7-A112-0003931BEA4A@uci.edu> Mime-Version: 1.0 (Apple Message framework v552) Content-Type: text/plain; charset=US-ASCII; format=flowed Content-Transfer-Encoding: 7bit X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 41EF57D1B4 for ; Mon, 2 Jun 2003 22:44:25 +0100 (BST) X-Received: from smtp1.es.uci.edu (smtp1.es.uci.edu [128.200.80.3]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B50E07D0F3 for ; Mon, 2 Jun 2003 22:44:23 +0100 (BST) X-Received: from uci.edu (bs2-23-kavita-226.bio.uci.edu [128.200.23.226]) by smtp1.es.uci.edu (8.12.8/8.12.5) with ESMTP id h52Ln0Wu015976 for ; Mon, 2 Jun 2003 14:49:00 -0700 X-To: dros@net.bio.net X-Mailer: Apple Mail (2.552) Date: Thu, 5 Jun 2003 15:30:22 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, Has anyone had any success with the ends-out method from Kent Golics March 2003 paper, as opposed to the ends in method? Or any other advice? I'm about to start designing the vector and I'd like to use the method that seems to be working the best for everyone! Thanks Louise Parker --- .