From owner-drosophila@net.bio.net Tue Jun 27 23:00:00 1995
Path: biosci!LUXOR.LATROBE.EDU.AU!gendjc
From: gendjc@LUXOR.LATROBE.EDU.AU
Newsgroups: bionet.drosophila
Subject: vitelline removal
Date: 27 Jun 1995 23:52:14 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <9506280650.AA14752@luxor.latrobe.edu.au>
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(sorry if this is a repeat. We've been having a spot of bother)

Hi all,
        I'm having fearful trouble removing the vitelline membrane from
D. serrata (melanogaster group) embryos. Simulans is fine. I'm shaking in
a 50/50 soln of n-heptane and 80% ethanol. If I use 100% methanol it
makes no difference. I get very few falling out of the organic layer.
Any suggestions?

thanks

David Clancy
genetics
la Trobe Uni.
Melbourne, Australia



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