From owner-biophysics@net.bio.net Thu Apr 02 23:00:00 1998
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From: murphy_r@licre.ludwig.edu.au (Roger Murphy)
Newsgroups: bionet.molbio.methds-reagnts,bionet.biophysics
Subject: Re: assay for free cysteines
Date: Fri, 03 Apr 98 06:37:37 GMT
Organization: Ludwig Institute for Cancer Research
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How big is your protein?  Ellmans reagent is certainly good for multiply 
bridged peptides of MW ~3000-4000, but if you're dealing with a large protein 
it may not penetrate any hydrophobic patches.

Roger
In article <35237E42.4625143F@mrc-lmb.cam.ac.uk>, Yu Wai Chen 
<ywc@mrc-lmb.cam.ac.uk> wrote:
>Dear all,
>
>I would like to know if the 6 cysteines of my protein is
>disulphide-bridged or not.  Can some one point me to a good reference or
>protocol how to achieve this?  I've heard that we can use Ellman's
>reagent to do it...  Is Ellman's reagent good for buried cysteines as
>well?  Thanks.
>
>-- 
>===================================================================
>Yu Wai CHEN, Ph.D. ..................  email: ywc@mrc-lmb.cam.ac.uk
> Centre for Protein Engineering,              tel: 44-(1223) 402148
> MRC Centre, Cambridge  CB2 2QH, U.K.         fax: 44-(1223) 402140
> WWW homepage: http://www.mrc-cpe.cam.ac.uk/people/wai.html



Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084
Australia.

Tel  61-3-94965463
Fax  61-3-94965436
Email murphy_r@licre.ludwig.edu.au

.