From owner-celegans@net.bio.net Thu Mar 06 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!arclight.uoregon.edu!dispatch.news.demon.net!demon!news.maxwell.syr.edu!news.cis.ohio-state.edu!nntp.sei.cmu.edu!fs7.ece.cmu.edu!casaba.srv.cs.cmu.edu!das-news2.harvard.edu!oitnews.harvard.edu!hpngsv01.mgh.harvard.edu!atgw-wel-800d-1-c14.mgh.harvard.edu!user From: patterson@frodo.mgh.harvard.edu (Garth Patterson) Newsgroups: bionet.celegans Subject: Re: Clean antibodies Date: 7 Mar 1997 12:26:58 GMT Organization: Massachusetts General Hospital Distribution: world Message-ID: References: <01IG5A45TLW88ZLRR7@vms.cis.pitt.edu> NNTP-Posting-Host: atgw-wel-800d-1-c14.mgh.harvard.edu Lines: 12 It seems to me that your problem should not be specific to goat anti-mouse or rabbit anti-mouse, if the problem is parasitic infection; that is goat anti-rabbit, for example would be as likely to give spurious reactivity. So I wonder if the problem is likely to be a supplier-specific problem--some suppliers may have better animal husbandry practices. Also, the Miller and Shakes chapter in the Red Worm Book recommends inquiring if the animals have had worms--I guess if your supplier says "Huh?", you can go elsewhere... All that said, I don't know who is good for Goat anti-mouse, but we have used goat anti-rabbit antibodies from Jackson ImmunoResearch Labs with good success. .