From owner-dros@hgmp.mrc.ac.uk Wed Oct 29 14:35:57 2003 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id C09F17D529 for ; Wed, 29 Oct 2003 14:35:56 +0000 (GMT) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3A7677D3EA for ; Wed, 29 Oct 2003 14:35:56 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 268C27D529; Wed, 29 Oct 2003 14:35:54 +0000 (GMT) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B99B97D302 for ; Tue, 28 Oct 2003 22:57:02 +0000 (GMT) Received: from horus.isnic.is (horus.isnic.is [193.4.58.12]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2F3FB7D142 for ; Tue, 28 Oct 2003 22:56:58 +0000 (GMT) Received: from proxy.google.com ([216.239.37.5]) by horus.isnic.is (8.12.9p2/8.12.9/isnic) with ESMTP id h9SMur9j041470 for ; Tue, 28 Oct 2003 22:56:55 GMT (envelope-from news@google.com) Received: from 10.7.0.80 by Google Production Router with ESMTP id h9SMtHfk027653 for ; Tue, 28 Oct 2003 14:55:17 -0800 Received: (from news@localhost) by Google Production with id h9SMtHeF001503 for bionet-drosophila@moderators.isc.org; Tue, 28 Oct 2003 14:55:17 -0800 To: bionet-drosophila@moderators.isc.org From: Luanhaojiang@hotmail.com (Haojiang) Newsgroups: bionet.drosophila Subject: How to avoid autofluorescence when dissect the brian? Organization: http://groups.google.com Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1067381716 1501 127.0.0.1 (28 Oct 2003 22:55:16 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: Tue, 28 Oct 2003 22:55:16 +0000 (UTC) Date: Wed, 29 Oct 2003 14:35:54 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, dear all, When I tried to dissect the brain and ventral nerve cord of the flies, frequently I got quite some autofluorescence in the central nervous system when I looked them under confocal microdcope. Any one please give some suggestions on how to avoid this? Thank you very much! luanh@nimh.nih.gov .