From owner-dros@hgmp.mrc.ac.uk Thu Jan 25 16:27:54 2001 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B646017B44; Thu, 25 Jan 2001 16:27:52 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 4332417B3F; Thu, 25 Jan 2001 16:27:50 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id CE5F317B16; Thu, 25 Jan 2001 16:21:17 +0000 (GMT) Received: from inside.sickkids.on.ca (mail.sickkids.on.ca [142.20.67.51]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 290D3415D5 for ; Thu, 25 Jan 2001 16:20:56 +0000 (GMT) Received: from resultra.sickkids.on.ca (resultra.sickkids.on.ca [142.20.140.100]) by inside.sickkids.on.ca (8.10.0/8.10.0) with ESMTP id f0PGTbZ01623 for ; Thu, 25 Jan 2001 11:29:37 -0500 Received: from [142.20.154.232] (julie-s-g4.sickkids.on.ca [142.20.154.232]) by resultra.sickkids.on.ca (8.9.3/8.9.3) with ESMTP id LAA05992 for ; Thu, 25 Jan 2001 11:13:35 -0500 (EST) X-Sender: jbrill@resmail.sickkids.on.ca Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: bionet-drosophila@net.bio.net From: "Dr. Julie Brill" Subject: Research Technologist II position Newsgroups: bionet.drosophila Date: Thu, 25 Jan 2001 16:27:50 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Please bring the following advertisement to the attention of any excellent candidates: RESEARCH TECHNOLOGIST II POSITION Position status: Full-time, fixed term Available: Immediately Salary: Commensurate with experience Description of the Position: Working with a starting investigator in a newly created laboratory in the Developmental Biology program, you will carry out experiments addressing cytoskeletal-membrane interactions crucial for completing cell division. You will use your knowledge of the following techniques to study the role of a lipid kinase in Drosophila germ cell cytokinesis: - Molecular biology (PCR, cloning, sequencing, Northern and Southern blots), - Protein biochemistry (Western blots, immunoprecipitation, affinity chromatography) - Drosophila molecular genetics (genetic crosses, production of transgenic flies, RNA in situ hybridization), - Tissue culture (Drosophila cells), - Cytology (phase microscopy, immunofluorescence) - Yeast molecular biology (two-hybrid analysis) Your duties will include: maintaining the lab in smooth running order by organizing lab stock collections (Drosophila, bacteria, yeast, tissue culture cells, antibodies, plasmids), supervising the maintenance of Drosophila stocks, ordering fly food and other reagents, and providing initial training for new members of the laboratory. The ability to maintain an independent research project would be a definite asset. Qualifications: You are a highly motivated person interested in learning new techniques. You are well organized and possess excellent interpersonal skills. You are able to work independently as well as in a team. Ideally, your training will include a completed B.Sc. or M.Sc. (preferred) in biochemistry, molecular biology, genetics or a related discipline, combined with a minimum of one year experience in a similar setting. Deadline: February 2, 2001 _______________________ Julie A. Brill, Ph. D. Research Institute The Hospital for Sick Children Rm. 7148 Elm Wing 555 University Avenue Toronto, ON M5G 1X8 CANADA Phone: (416) 813-8863 Email: jbrill@sickkids.ca ______________________ Julie A. Brill, Ph. D. Research Institute The Hospital for Sick Children Rm. 7148 Elm Wing 555 University Avenue Toronto, ON M5G 1X8 CANADA Phone: (416) 813-8863 Email: jbrill@sickkids.ca .