From owner-dros@hgmp.mrc.ac.uk Thu Jan 31 18:38:31 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7265E17AE7 for ; Thu, 31 Jan 2002 18:38:30 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7267617AF2 for ; Thu, 31 Jan 2002 18:38:28 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 8D01F17AE7; Thu, 31 Jan 2002 18:38:27 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 401C917AD7 for ; Thu, 31 Jan 2002 16:42:44 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5A97717AC8; Thu, 31 Jan 2002 16:42:43 +0000 (GMT) To: bionet-drosophila@net.bio.net From: janssens@pharm.sunysb.edu ("janssens") Newsgroups: bionet.drosophila Subject: non-radioactive labelling Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <3C5973F6.BC9CEC5E@pharm.sunysb.edu> MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2286E17AD7 for ; Thu, 31 Jan 2002 16:42:40 +0000 (GMT) X-Received: from pharmsci2.pharm.sunysb.edu (pharmsci2.pharm.sunysb.edu [129.49.145.197]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9A4BA17AC8 for ; Thu, 31 Jan 2002 16:42:38 +0000 (GMT) X-Received: from pharm.sunysb.edu (042-202.bio.sunysb.edu [129.49.42.202]) by pharmsci2.pharm.sunysb.edu (Netscape Messaging Server 3.62) with ESMTP id 663 for ; Thu, 31 Jan 2002 11:42:32 -0500 X-Reply-To: janssens@pharm.sunysb.edu X-Mailer: Mozilla 4.79 (Macintosh; U; PPC) X-Accept-Language: en X-To: dros@net.bio.net Date: Thu, 31 Jan 2002 18:38:27 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi all What is the state of the art of using non-radioactive labelling methods for Drosophila Southerns? Is the sensitivity already comparable with radioactive labelling? Any good protocols, tips, or are we all still using radioactivity? Hilde ----------------------------- Hilde Janssens, PhD SUNY at Stony Brook Center for Developmental Genetics CMM Building, Room 495 Stony Brook, NY 11794-5140 phone: 1 631 632 1477 fax: 1 631 632 1692 ----------------------------- --- .