From owner-drosophila@net.bio.net Sat Aug 24 23:00:00 1996 Path: biosci!TECHUNIX.TECHNION.AC.IL!zlev From: zlev@TECHUNIX.TECHNION.AC.IL (Zeev Lev) Newsgroups: bionet.drosophila Subject: Re: pWHL vector Date: 25 Aug 1996 01:20:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19960825082244.00682fe0@tx.technion.ac.il> NNTP-Posting-Host: net.bio.net At 07:57 AM 8/23/96 -0700, you wrote: >Dear Netters >I am using the vector pWHL for p element mediated germ line >transformation. It is supposed to be a derivative of vector pW8 and >constructed by Dr.F. Michiels, Martinsried, West Germany. I would like to >know the source of lacZ in this vector to design primers in order to check >orientation of my inserts. I would appreciate if any body can give me this >information or reference for pW8. >Thanks >Venky (Email:phage@umich.edu) Information regarding construction, map, and sequence of pP{W8} are found in the following URL`s: http://morgan.harvard.edu/htbin-post/moseg-rpt.script?ms00000478 http://biology.queensu.ca/~miseners/vector_pages/Dros_vectors.html Zeev Dr. Zeev Lev Tel: 972-4-829 4215 Department of Biology Fax: 972-4-822 5153 Technion E-mail: zlev@tx.technion.ac.il Haifa 32000 Israel .