From owner-dros@hgmp.mrc.ac.uk Mon Apr 16 15:29:21 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D117B17AB5 for ; Mon, 16 Apr 2001 15:29:20 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 477D017AC0 for ; Mon, 16 Apr 2001 15:29:19 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id F056317AB5; Mon, 16 Apr 2001 15:29:15 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2858B17ABB for ; Sun, 15 Apr 2001 18:02:37 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 3D8D317AB3; Sun, 15 Apr 2001 18:02:34 +0100 (BST) To: bionet-drosophila@net.bio.net From: delorenzo@zi.biologie.uni-muenchen.de (David De Lorenzo) Newsgroups: bionet.drosophila Subject: Balancers Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4C96817ABB for ; Sun, 15 Apr 2001 18:02:32 +0100 (BST) X-Received: from mailhost.zi.biologie.uni-muenchen.de (unknown [141.84.243.18]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0074D17AB3 for ; Sun, 15 Apr 2001 18:02:18 +0100 (BST) X-Received: from [141.84.244.18] (unknown [141.84.244.18]) by mailhost.zi.biologie.uni-muenchen.de (Postfix) with ESMTP id E154F7F011 for ; Sun, 15 Apr 2001 19:00:57 +0200 (MEST) X-Sender: delorenzo@mailhost.zi.biologie.uni-muenchen.de X-To: dros@net.bio.net Date: Mon, 16 Apr 2001 15:29:15 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I am looking for good balancers to create isogenic lines in Drosophila melanogaster. I have been looking at the bibliography, but the problem is, there are hundreds of balancers, each one of them good enough for studying a specific region in a chromosome, because they reduce effectively the recombination in that region. But I would like to know which ones are the most effective general suppressors of recombination. It looks like the most efficient balancers are FM3 for the X chromosome, SM5 and CyO for the 2nd chromosome, and TM6B for the 3rd chromosome. My questions are: are these really the best balancers? if yes, what would be the best markers that should go with each of this balancers? Any suggestions? Please email the answers to delorenzo@zi.biologie.uni-muenchen.de Many thanks, David -- _______________________________________________________________________ Dr. David De Lorenzo Office: (+49 89) 5902 330 Institute of Zoology-LMU Lab: (+49 89) 5902 326 LuisenStr. 14 Fax: (+49 89) 5902 474 80333 Munich - Germany delorenzo@zi.biologie.uni-muenchen.de _______________________________________________________________________ --- .