From owner-celegans@net.bio.net Wed Aug 27 23:00:00 1997
Path: biosci!lamg.com!Tritech_Research
From: Tritech_Research@lamg.com (Tritech Research)
Newsgroups: bionet.celegans
Subject: Re: OP50 follow up
Date: 27 Aug 1997 17:31:58 -0700
Organization: Los Angeles Macintosh Group BBS
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Sender: daemon@net.bio.net
Distribution: world
Message-ID: <20724798.10153115@lamg.com>
Reply-To: Tritech_Research@lamg.com
NNTP-Posting-Host: net.bio.net

Hi,

 has anyone ever tried to transform the OP50 strain with any plasmid 
containing an antibiotic resistant marker?

Renaud
----------
Yes - it transforms easily with pUC19 / Bluescipt type plasmids
and is then resistant to Amp as expected.  However, I believe it is
"blue" with or without a plasmid, since it is wild-type for B-gal,
and it doesn't transform as efficiently as MC1061, DH5a, etc.

Andy Papp
tritech_research@LAMG.COM

.