From owner-celegans@net.bio.net Mon Aug 02 12:58:00 1999
Path: biosci!biosci!not-for-mail
From: Plxcjm@nottingham.ac.uk ("CHRIS.J.MEE")
Newsgroups: bionet.celegans
Subject: dsRNA Soaking
Date: 2 Aug 1999 06:57:54 -0700
Organization: Life Science, Univ of Nottingham
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As a first year PhD student who is currently working with dsRNA
interference in C.elegans i was interested to see in some of the
abstracts at the 1999 International Worm Meeting that a number of
people were using dsRNA soaking as a method of RNAi instead of
microinjection.
I dont seem to be able to find anything further on the technique so i
was hopeing that someone may be able to fill me in on how its done
and how successful it is compared to microinjection of dsRNA.
I would also like to know if anybody has a good gene to use as a
positive control in dsRNA injections as the genes i am looking at
have not been used before and so i have no idea if they give an
obvious phenotype. It may be that my injection technique needs work
so a positive control with a good phenotype would help.

Any help would be great
Chris Mee
University of Nottingham
plxcjm@nottingham.ac.uk
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