From owner-dros@hgmp.mrc.ac.uk Mon Apr 16 15:28:46 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 876B517AC0 for ; Mon, 16 Apr 2001 15:28:44 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0687217AF3 for ; Mon, 16 Apr 2001 15:28:39 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 0AB4F17ADE; Mon, 16 Apr 2001 15:28:34 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 5D33B17ABA for ; Sun, 15 Apr 2001 07:56:46 +0100 (BST) Received: from lsmls02.we.mediaone.net (lsmls02.we.mediaone.net [24.130.1.15]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D352617AB7 for ; Sun, 15 Apr 2001 07:56:31 +0100 (BST) Received: from TritechResearch.com (we-24-160-36-125.we.mediaone.net [24.160.36.125]) by lsmls02.we.mediaone.net (8.11.1/8.11.1) with ESMTP id f3F6uQi20253; Sat, 14 Apr 2001 23:56:26 -0700 (PDT) Message-ID: <3AD94616.6AC8AC65@TritechResearch.com> From: Andrew Papp Reply-To: apapp@TritechResearch.com Organization: Tritech Research X-Mailer: Mozilla 4.75 (Macintosh; U; PPC) X-Accept-Language: en,pdf MIME-Version: 1.0 To: s.chyb@ic.ac.uk, bionet-drosophila@net.bio.net Subject: Re: transfecting S2 cells Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit Newsgroups: bionet.drosophila Date: Mon, 16 Apr 2001 15:28:34 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Can anyone share their experiences transfecting S2 cells? Did anybody have much luck with electroporation and if so could share a protocol? Thanks, Sylwek -- Dr. Sylwester Chyb Biology Imperial College University of London Wye Campus Wye, Kent TN25 5AH United Kingdom -------------------------- Dear Sylwek, Yes, electroporation works well with S2 cells. We make a very user-friendly, inexpensive, hand-held electroporator called The Cloning Gun. A lab in the U.K. recently ordered the MammoZapper version (the Cloning Gun for mammalian cells) and was able to get 20% of S2 cells transfected with it. Here is some information about the Cloning Gun, including links to review articles: http://www.TritechResearch.com/ click on Cloning Gun, under Hightlights. You'll also notice great inexpensive heating/cooling incubators for growing temperature-sensitive mutants, microinjection equipment, and lot of other items designed for Drosphila genetics. The person doing the S2 transfections is Jon Radford, in Julian Dow's lab at U of Glasgow. His e-mail address is: "Jonathan Radford" And I will be happy to asnwer any questions, if contacted at the e-mail or telephone number below. Best regards, Andrew -- Andrew Papp Tritech Research, Inc. 2961 Veteran Avenue Los Angeles, CA 90064 Tel: (310)446-4460 Fax: (310)475-1533 Orders: (800)7-333-TRI apapp@TritechResearch.com http://www.TritechResearch.com/ .