From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.diagnostics Subject: sample collection Date: 4 Apr 1995 14:36:13 -0700 Organization: University of Arkansas Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <1FA3363D96@mercury.uark.edu> I would like to poll the this newsgroup as to what methods others might recommend for sample collection. We need to collect microliter amounts of blood for PCR analysis. Unfortunately, the samples should be most easily collected by untrained animal handlers. We have looked into cap tubes and the micro-vacutainer type collection tubes but all of these require using a lancet and then collection from skin surfaces. It would be really nice if there was a vacutainer/needle system that collects only 10-100 microliters but those systems start at around 2 ml. Since we are using PCR we are very concerned that with surface collection there can be cross-contamination from handlers hands and from dander on the skin surface. We also don't want to have to design something. Does anyone have any suggestions where we might look?? //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// .