From owner-dros@hgmp.mrc.ac.uk Thu Feb 21 13:46:38 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DA4C417AC3 for ; Thu, 21 Feb 2002 13:46:34 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id F41AF17AC4 for ; Thu, 21 Feb 2002 13:46:30 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id E421317AC3; Thu, 21 Feb 2002 13:46:29 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9AECE17B78 for ; Thu, 21 Feb 2002 09:41:42 +0000 (GMT) Received: from chx400.switch.ch (chx400.switch.ch [130.59.10.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 520C217B76 for ; Thu, 21 Feb 2002 09:41:41 +0000 (GMT) Received: from smtp3.google.com ([216.239.33.28]) by chx400.switch.ch with esmtp (Exim 3.20 #1) id 16dpjQ-0000e8-00 for bionet-drosophila@moderators.isc.org; Thu, 21 Feb 2002 10:41:40 +0100 Received: from sjins2.sj.google.com (proxy.google.com [216.239.35.5]) by smtp3.google.com with ESMTPœ id BAA01563 for ; Thu, 21 Feb 2002 01:41:38 -0800 Received: from sjmm36.sj.google.com (sjmm36.prod.google.com [10.6.39.36]) by sjins2.sj.google.com (8.9.3/8.9.3) with ESMTP id BAA02468 for ; Thu, 21 Feb 2002 01:41:37 -0800 Received: (from news@localhost) by sjmm36.sj.google.com (8.9.3/8.9.3) id BAA16107 for bionet-drosophila@moderators.isc.org; Thu, 21 Feb 2002 01:41:36 -0800 To: bionet-drosophila@moderators.isc.org From: daulny@cnrs-orleans.fr (anne daulny) Newsgroups: bionet.drosophila Subject: drosophila nuclear extract Organization: http://groups.google.com/ Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1014284496 16106 127.0.0.1 (21 Feb 2002 09:41:36 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: 21 Feb 2002 09:41:36 GMT Date: Thu, 21 Feb 2002 13:46:29 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk I'm a PhD student and I need some help. I don't know how to make a drosohila protein embryo extract for an immunoprecipitation assay. I'd be very grateful if someone can give me a protocol. Thank you for your help. Anne Daulny Tel : (33)02 38 25 55 39 Fax : (33)02 38 63 15 17 e-mail : daulny@cnrs-orleans.fr C.N.R.S. Centre de biophysique Moléculaire Rue Charles SADRON 45071 ORLEANS Cedex 2 FRANCE .