From owner-metabolic-reg@net.bio.net Wed Jan 29 22:00:00 1997 Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!hermod.uio.no!nntp.uio.no!data.ramona.vix.com!sonysjc!sonybc!newsjunkie.ans.net!newsfeeds.ans.net!rcogate.rco.qc.ca!altitude!usenet From: "Achim Recktenwald, PhD" Newsgroups: bionet.glycosci,bionet.molbio.proteins,de.sci.biologie,sci.bio.misc,bionet.metabolic-reg,bionet.molbio.methds-reagnts,bionet.biophysics,bionet.general, Subject: enzyme kinetics with polymer substrates Date: Thu, 30 Jan 1997 12:54:10 -0500 Organization: IBEX Technologies, Inc., Biochemistry, 5485 Pare, Montreal, PQ, H4P 1P7, Canada Lines: 31 Message-ID: <32F0E040.3EFC@ibex.ca> NNTP-Posting-Host: ibex.hip.cam.org Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) CC: achim@ibex.ca Xref: biosci bionet.glycosci:948 bionet.molbio.proteins:9876 sci.bio.misc:7185 bionet.metabolic-reg:970 bionet.molbio.methds-reagnts:54174 bionet.biophysics:2596 bionet.general:25388 Hi everybody, I am working with enzymes using polysaccharides as substrate. Each polysaccharide molecule contains several 'substrate-sites' at which it is cut into subsequently smaller fragments. With all likelihood the affinity of the enzymes for these fragments differs, e.g., the Km and/or Vmax-value is not the same, as for the undigested polymer. I am now looking for publications describing the enzyme kinetics and experiments concerning the enzyme mechanism of these kind of enzymes. Since there are many enzymes with a similar behavior, e.g., amylases, DNA restriction enzymes, and so on, I am hoping that there is some literature availabe. Any help would be appreciated. Thanks a lot in advance, Achim P.S. Our service-provider seems to have occasional problems with the news-server. Please send me an email-copy of any answer. ___________________________ Achim Recktenwald, PhD IBEX Technologies, Inc. 5485 rue Pare Montreal, Quebec, H4P 1P7 Canada achim@ibex.ca .