From owner-cellbiol@net.bio.net Sat Dec 07 22:00:00 1996
Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!paladin.american.edu!news.jhu.edu!news
From: Scott Walsh <swalsh@bme.jhu.edu>
Newsgroups: bionet.cellbiol
Subject: DNA lebel
Date: Sun, 08 Dec 1996 16:53:29 -0500
Organization: The Johns Hopkins University
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Hi..
Can anyone tell me what is the best way to label a circular plasmid
(probably supercoiled) with radioactive label.  I tried nick translation
but the labeled mixture after passing through sephadex column and
running a gel seems to have all oligonucleotides.  I need to keep the
DNA as a circular plasmid. Thanks..
Please reply at kroy@bme.jhu.edu

Thanks..

Krish Roy

.