From owner-dros@hgmp.mrc.ac.uk Fri Apr 25 14:06:50 2003 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 8640F7D562 for ; Fri, 25 Apr 2003 14:06:49 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 836C47D24F for ; Fri, 25 Apr 2003 14:06:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 4D24B7D533; Fri, 25 Apr 2003 14:06:42 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 81AF77D28E for ; Thu, 24 Apr 2003 14:07:12 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C2F387D1BE; Thu, 24 Apr 2003 14:07:06 +0100 (BST) To: bionet-drosophila@net.bio.net From: Christian.Wolf@intervet.com ("Wolf, C (Christian)") Newsgroups: bionet.drosophila Subject: Transposase Transgenic Flies Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <216DB563CAF3D4118FA40002A52844D7AFC4D9@shmn04> MIME-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 5E9DA7D28E for ; Thu, 24 Apr 2003 14:07:05 +0100 (BST) X-Received: from ns.intervet.com (ns.intervet.com [212.153.37.135]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 036E87D31D for ; Thu, 24 Apr 2003 14:07:03 +0100 (BST) X-Received: (from root@localhost) by ns.intervet.com (8.9.3c/8.6.12) id PAA11258 for ; Thu, 24 Apr 2003 15:07:03 +0200 (MET DST) X-Received: by ns.intervet.com (TUNIX txp2/smap) for id sma009462; Thu, 24 Apr 03 15:05:25 +0200 X-Received: by inbexc0.inb.intra with Internet Mail Service (5.5.2653.19) id ; Thu, 24 Apr 2003 15:05:21 +0200 X-To: "'dros@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2653.19) Date: Fri, 25 Apr 2003 14:06:42 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I have a general question to the community: To become transgenic flies normally helper vectors coding for transposase are used. 1) Does D. melanogaster WT encode for transposase? 2) Do theses P-elements occur in D. melanogaster Wt? 3) From which organism does the transposase encoded by Helper Plasmids come from? 4) Is there literature (reviews) about this topic? Thank you in advance Christian -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- --- .