From owner-diagnostics@net.bio.net Fri Apr 21 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: lambda phage mabs Date: 22 Apr 1995 00:06:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On 21 Apr 1995, Robert Burns wrote: > > Hi all, > have any of you had a go at generating antibodies using > lambda phage expression vectors. I know that one main advantage > is the numbers of antibodies generated but what are the disadvantages? > Thirdly does anyone know of an easy-to-use kit which can be used for > this technology? > > Thanks > > Robert > > > > > > Robert Burns > The main problem of this method is the low affinity of the resulting antibodies. I did not have direct experience with the method but we keep it in the back of our minds as a viable strategy to generate antibodies, while combining it with an approach to ensure that the affinity will be usably high. As to a kit: I think Pharmacia offers a kit. I am not sure and I will get back after looking at my papers. You could also contact the guys at Cambridge: they have invented it all. Falk Fish, Tel-Aviv, Israel .