From owner-celegans@net.bio.net Tue Aug 26 23:00:00 1997 Path: biosci!FALCO.GEOBIO.ELTE.HU!fodoran From: fodoran@FALCO.GEOBIO.ELTE.HU Newsgroups: bionet.celegans Subject: Re: OP50.1 (fwd) Date: 26 Aug 1997 23:39:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 148 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleauges, Your friend Andras has probably a starting Altzheimer or a other kind of brain lesion. I changed the word SENSITIVE to RESISTANT!!!! Sorry! So OP50 PROVED EXTREMELY SENSITIVE, (not resistant) TO ALL THE 24 antibiotics teste. Faithfuly yours, Andras (without his gang). ======================================================================== Dr. Andras Fodor e-mail: fodoran@ludens.elte.hu Associate Professor or: fodoran@falco.elte.hu Eotvos University Phone: +36.1.266.1296 Department of Genetics +36.1.267.5444 ext. 2348 Budapest, Muzeum krt. 4/A FAX: +36.1.266.2694 H-1088 HUNGARY ======================================================================== ---------- Forwarded message ---------- Date: Tue, 26 Aug 1997 20:37:01 +0200 (MET DST) From: fodoran@falco.geobio.elte.hu To: "Bionet.Celegans.Mailgroup" , Netbionet Celegans , Theresa Stierngale , Tibor Vellai , ryder@WPI.EDU Cc: FODORAN@FALCO.elte.hu Subject: Re: OP50.1 On Tue, 26 Aug 1997 fodoran@ludens.elte.hu wrote: Dear Liz, Dear Newsgroup, Although we are going to send these data to the next issue of the Newsletter, let us share our results with you: ANTIBIOTICS SENSITIVITY OF OP50. Csaba Bognar(2), Tibor Vellai(1) and Andras Fodor(1), 1. Dept. Genetics, Eotvos University, H-1088 Budapest, Muzeum krt. 4/A, Hungary 2. Bela Johann National Institute of Public Health in Hungary, Department of Bacteriology, (OKI), H-1097 Budapest, Gyali ut 2-6, Hungary. E. coli OP50 is used practically by everybody in the C. elegans Community as natural food for C.elegans. Our substrain what we use was kindly provided by Ian Hope in 1995. We have tested its antibiotics sensitivity, by the paper disc method routinly used the OKI. We used 30 ug/ml of each antibiotics tested. The filter paper discs are out on the center of the NGM plate seeded by OP bacteria. An inhibition zone develops, if the antibiotics is effective against the baccteria under test. Conventionally, if the diameter oif the inhibition zone is smaller then 2 cm, we conclude that the bacteria is resistant to the antibiotics tested. We tested 24 antibiotics, as follows: gentamycin; tobramycin; sumetrolim; chloramphenicol; tetracycline; ampicyllin; karbenicillin; cefalexine; cefamandol; cefuroxime; amykacin; netylmycine; azlocilline; mezlocillin; ceftriaxone; cefatoxim; cefroperazon; ofloxacin; pestloxacin; cyprofloxacim; ceftazidin; cefaclor; augmentin; and kanamycin. (Some of the names are probably written in very Hungarian; we promise the correct them in the Newsletter article). Well, we found that OP50 was completely SENSITIVE to all these antibiotics. I hope these data help Liz and other fellow Colleauges to work with OP50. Faithfully yours, Andras and his gang. > From: MX%"ryder@WPI.EDU" 14-AUG-1997 16:32:41.88 > To: MX%"bionet-news@dl.ac.uk" > CC: > Subj: Re: OP50 Antibiotic resistance > > Return-Path: > Received: from mserv1.dl.ac.uk by ludens.elte.hu (MX V4.2 VAX) with SMTP; Thu, > 14 Aug 1997 16:32:41 +0100 > Received: by mserv1.dl.ac.uk id PAA03450 (8.6.13/5.3[ref postmaster@dl.ac.uk] > for dl.ac.uk from server-daemon@dl.ac.uk); Thu, 14 Aug 1997 15:18:51 > +0100 > Resent-From: > Received: by mserv1.dl.ac.uk id PAA03350 (8.6.13/5.3[ref postmaster@dl.ac.uk] > for dl.ac.uk from server-daemon@dl.ac.uk); Thu, 14 Aug 1997 15:17:51 > +0100 > Received: by mserv1.dl.ac.uk id PAA03112 (8.6.13/5.3[ref postmaster@dl.ac.uk] > for dl.ac.uk from news@dl.ac.uk); Thu, 14 Aug 1997 15:16:17 +0100 > Date: 14 Aug 1997 06:56:36 -0700 > Resent-Date: Thu, 14 Aug 97 15:18:40 UT > Message-ID: <97814151840.~INN-LWAa00195.bionet-news@dl.ac.uk> > Resent-Message-ID: > Precedence: list > From: ryder@WPI.EDU (Elizabeth Ryder) > Reply-To: ryder@WPI.EDU (Elizabeth Ryder) > Sender: "bionet.celegans mail newsgroup" > To: "bionet.celegans mail newsgroup" > Subject: Re: OP50 Antibiotic resistance > Comments: List problems/queries to > Comments: To mail both the group and netnews send to (celegans@dl.ac.uk) > X-Article-Number: bionet.celegans Msg # 1646 > X-Listpath: bionet-news > X-Mailer: MXT V 12.16.1 > > All I know is that it's NOT resistant to Amp or Kan. > > Liz Ryder > Worcester Polytechnic Institute > Dept. of Biology and Biotechnology > Worcester, MA 01609 > email: ryder@wpi.edu > phone: 508-831-6011 > fax: 508-831-5936 > > On Wed, 13 Aug 1997, John M. Hawdon wrote: > > > Hi; > > Anyone off hand know whether OP50 is resistant to any antibiotics? > > > > Thanks > > > > John Hawdon > > > > > > > > ======================================================================== Dr. Andras Fodor e-mail: fodoran@ludens.elte.hu Associate Professor or: fodoran@falco.elte.hu Eotvos University Phone: +36.1.266.1296 Department of Genetics +36.1.267.5444 ext. 2348 Budapest, Muzeum krt. 4/A FAX: +36.1.266.2694 H-1088 HUNGARY ======================================================================== .