From owner-cellbiol@net.bio.net Sun Jan 12 22:00:00 1997 Path: biosci!PO-BOX.MCGILL.CA!nha9 From: nha9@PO-BOX.MCGILL.CA (by way of nha9@po-box.mcgill.ca (Nhuan Ha)) Newsgroups: bionet.cellbiol Subject: How to get heme into animal cells? Date: 13 Jan 1997 15:01:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I'm working with a yeast protein whose activity is regulated by heme. When I transfect appropriate plasmid encoding this yeast protein into Hela cells, there is no activity. There may be several reasons for this but maybe there is not enough endogeneous heme in this Hela cell line or something like that. I wonder how I could increase heme level inside the cells. In yeast, one can supply heme by using a precursor called ALA which will be taken by the yeast cells and consequently converted to heme inside the cells. I also wonder if there exists a receptor for heme? Any help will be appreciated. Thank you. Nhuan Ha nha9@po-box.mcgill.ca .