From owner-dros@hgmp.mrc.ac.uk Thu Apr 24 13:59:20 2003 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 6F2C67D31D for ; Thu, 24 Apr 2003 13:59:19 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8D3E47D313 for ; Thu, 24 Apr 2003 13:59:18 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 5FA187D28E; Thu, 24 Apr 2003 13:59:12 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8B61E7D12B for ; Thu, 24 Apr 2003 10:09:58 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id A672C7D2EB; Thu, 24 Apr 2003 10:09:52 +0100 (BST) To: bionet-drosophila@net.bio.net From: Christian.Wolf@intervet.com ("Wolf, C (Christian)") Newsgroups: bionet.drosophila Subject: Transposase Transgenic Flies Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <216DB563CAF3D4118FA40002A52844D7AFC4D7@shmn04> MIME-Version: 1.0 Content-Type: text/plain; charset="iso-8859-1" X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id AAE787D12B for ; Thu, 24 Apr 2003 10:09:51 +0100 (BST) X-Received: from ns.intervet.com (ns.intervet.com [212.153.37.135]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 585597D2EB for ; Thu, 24 Apr 2003 10:09:50 +0100 (BST) X-Received: (from root@localhost) by ns.intervet.com (8.9.3c/8.6.12) id LAA03974 for ; Thu, 24 Apr 2003 11:09:49 +0200 (MET DST) X-Received: by ns.intervet.com (TUNIX txp2/smap) for id sma002503; Thu, 24 Apr 03 11:08:01 +0200 X-Received: by inbexc0.inb.intra with Internet Mail Service (5.5.2653.19) id ; Thu, 24 Apr 2003 11:07:58 +0200 X-To: "'dros@net.bio.net'" X-Mailer: Internet Mail Service (5.5.2653.19) Date: Thu, 24 Apr 2003 13:59:12 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I have a general question to the community: To become transgenic flies normally helper vectors coding for transposase are used. 1) Does D. melanogaster WT encode for transposase? 2) Do theses P-elements occur in D. melanogaster Wt? 3) From which organism does the transposase encoded by Helper Plasmids come from? 4) Is there literature (reviews) about this topic? Thank you in advance Christian _____________________ Dr. Christian Wolf Akzo Nobel Intervet Innovation GmbH Drug Discovery / Target Discovery Zur Propstei 55270 Schwabenheim Germany Tel. +49 (0)6130 948 366 Fax. +49 (0)6130 948 517 Email: christian.wolf@intervet.com -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- --- .