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From: cathy.slack@ic.ac.uk (Cathy Slack)
Newsgroups: bionet.drosophila
Subject: Embryo in situ problems
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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Date: Mon,  6 Aug 2001 14:04:09 +0100 (BST)
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I have been having problems with in situ hybrisisation to embryos using
DIG-labelled RNA probes. I basically see a lot of non-specific labelling
and even see the probe hybridising to the nucleus! I don't think its my
probe as different probes give the same result. Has anyone ever
experienced this problem before and if so how do I rectify it??? I am
basically following a standard in situ protocol.

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