From owner-celegans@net.bio.net Mon Sep 15 23:00:00 1997
Path: biosci!FRODO.MGH.HARVARD.EDU!patterson
From: patterson@FRODO.MGH.HARVARD.EDU
Newsgroups: bionet.celegans
Subject: lysing worms for biochemistry
Date: 16 Sep 1997 09:00:25 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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I am interested in getting worm lysate for co-immunoprecipitation and
the like, and I am wondering if anyone has suggestions on preparing the
worms.  Is freeze-thawing adequate, or should we add sonication or other
mechanical disruption?

Garth Patterson
Ruvkun Lab

.