From owner-biophysics@net.bio.net Thu Apr 02 23:00:00 1998
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From: petter@mindspring.com (Petter)
Newsgroups: bionet.molbio.methds-reagnts,bionet.biophysics
Subject: Re: assay for free cysteines
Date: Thu, 02 Apr 1998 20:40:46 -0600
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In article <35237E42.4625143F@mrc-lmb.cam.ac.uk>, Yu Wai Chen
<ywc@mrc-lmb.cam.ac.uk> wrote:

>Dear all,
>
>I would like to know if the 6 cysteines of my protein is
>disulphide-bridged or not.  Can some one point me to a good reference or
>protocol how to achieve this?  I've heard that we can use Ellman's
>reagent to do it...  Is Ellman's reagent good for buried cysteines as
>well?  Thanks.
>

Ellman's will quantify cys residues that are both free and accessible, you
can always denature
 and quantify to  get the buried ones...



>-- 
>===================================================================
>Yu Wai CHEN, Ph.D. ..................  email: ywc@mrc-lmb.cam.ac.uk
> Centre for Protein Engineering,              tel: 44-(1223) 402148
> MRC Centre, Cambridge  CB2 2QH, U.K.         fax: 44-(1223) 402140
> WWW homepage: http://www.mrc-cpe.cam.ac.uk/people/wai.html

.