From owner-metabolic-reg@net.bio.net Tue Sep 03 23:00:00 1996
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From: Achim Recktenwald <achim@ibex.ca>
Newsgroups: bionet.molbio.proteins,de.sci.chemie,sci.bio.misc,sci.chem,bionet.metabolic-reg,bionet.molbio.methds-reagnts,bionet.biophysics,de.sci.misc
Subject: Bis-tris-propane: temp. coeff. (dpKa/dT) ??
Date: Wed, 04 Sep 1996 16:48:25 -0500
Organization: IBEX Technologies, Inc., Biochemistry, 5485 Pare, Montreal, PQ, H4P 1P7, Canada
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Hi all,

I am looking for the temperature coefficient(s) [dpKa/dT] of the
buffer-substance
'bis-tris-propane' = 1,3-bis[tris(hydroxymethyl)methylamino]propane 
for some enzyme kinetic experiments.  

According to the supplier (Sigma) it has the pKa-values:
pKa1=6.8 and pKa2=9.0 at T=25C.

Sigma doesn't know the dpKa/dT-value(s) either.
Can anybody help ?


Thanks,

Achim

.