From owner-biotechniques@net.bio.net Tue Dec 09 22:00:00 1997 Path: biosci!biosci!not-for-mail From: MMCCARTHY@biotechnet.com Newsgroups: bionet.journals.letters.biotechniques Subject: BioTechniques 24(1), January 1998 Date: 10 Dec 1997 08:41:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 153 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <01IR06PG2YJ69BVQ3Y@delphi.com> NNTP-Posting-Host: net.bio.net BioTechniques 24(1), January 1998 Table of Contents Benchmarks Rapid Amplification of Transposon Ends for the Isolation, Cloning and Sequencing of Transposon-Disrupted Chromosomal Genes E.M. Ribot, F.D. Quinn, X. Bai and J.J. Murtagh, Jr. Comparative PCR: An Improved Method to Detect Gene Amplification N. Brass, D. Heckel and E. Meese Adaptor PCR for the Specific Amplification of Unknown DNA Fragments H. Willems Use of T4 DNA Polymerase to Create Cohesive Termini in PCR Products for Subcloning and Site-Directed Mutagenesis A.J. Cooney Amplification of Short Tandem Repeat Loci: Performance of Different Polymerases and Radionucleotides B. Winnepenninckx and T. Backeljau Increased Yield of Tri- and Tetranucleotide Heterospecific Microsatellites from Unenriched Small-Insert Libraries K.D. Makova and J.C. Patton Vector-Swapping Strategy with an Exonuclease in Cloning and High Expression of Small PCR Products M. Obara and K. Yoshizato Universal Amplification of DNA Isolated from Small Regions of Paraffin-Embedded, Formalin-Fixed Tissue S.W. Faulkner and D.A. Leigh UV Irradiation of Polystyrene Pipets Releases PCR Inhibitors V. Linquist, C.A. Stoddart and J.M. McCune Screening for Positive Transfected Clones with Coexpressed Green Fluorescent Protein X. Li, N. Ngo, C. Hou, S. Cunningham, R.B. Zhang, Z. Li and C.-C. Huang Elimination of Internal Restriction Enzyme Sites from a Bacterial Luminescence (luxCDABE) Operon C.R. Voisey and F. Marincs Dye-Induced Denaturation of DNA Dissolved in Water B. Shieh, I.-J. Su, W.-J. Chuang and C. Li pBendBlue: Modification of the pBend System for Color Selectability S.J. Sperbeck and G.J. Wistow Nonradioactive UV Cross-Linking Assay for the Study of Protein-RNA Binding C. Vaquero, Y.-C. Liao and R. Fischer Higher Sensitivity of Denaturing Gradient Gel Electrophoresis than Sequencing in the Detection of Mutations in DNA from Tumor Samples M. Garcia-Delgado, C.J. Gonzalez-Navarro, M.C. Napal, C. Baldonado, J.L. Vizmanos and A. Gull Defogging Tissue Culture Lids for Viewing Cells M.G. Price, J.M. Schwartz Ebarb and E.G. Frank Internet On-Ramp Cyberspace for Biologists Short Technical Reports Comparison of Histologic Stains for Use in PCR Analysis of Microdissected, Paraffin-Embedded Tissues M.P. Burton, B.G. Schneider, R. Brown, N. Escamilla-Ponce and M.L. Gulley Coverslip Mounted-Immersion Cycled In Situ RT-PCR for the Localization of mRNA in Tissue Sections R. Ertsey and L.M. Scavo Mass Spectrometric Methods for Evaluating Point Mutations J.K. Lewis, J.R. Krone and R.W. Nelson Fluorescence-Based Protein Footprinting Using Histidine-Tagged Protein G.V. Rajendrakumar and S. Adhya Novel Vector for Generating RNAs with Defined 3-prime Ends and Its Use in Antiviral Strategies A. Schwienhorst and B.F. Lindemann Determination of Transgene Copy Number and Expression Level using Denaturing Gradient Gel Electrophoresis M.D. Ringel, W.F. Schwindinger, M. Saji, M.A. Zeiger and M.A. Levine Adaptation of Standard Spreadsheet Software for the Analysis of DNA Sequences N.R. McEwan and D. Gatherer Research Reports Denaturation Fingerprinting: Two Related Mutation Detection Methods Especially Advantageous for High G+C Regions Q. Liu, B.G. Weinshenker, D.M. Wingerchuk and S.S. Sommer Automated Recording of RNA Differential Display Patterns from Pig Granulosa Cells J. Motlik, J.W. Carnwath, D. Herrmann, V. Terletski, M. Anger and H. Niemann Product Application Focus Advantages of a New Taq DNA Polymerase in Multiplex PCR and Time-Release PCR C. Kebelmann-Betzing, K. Seeger, S. Dragon, G. Schmitt, A. Moricke, T.A. Schild, G. Henze and B. Beyermann New Products Index to Advertisers .