From owner-celegans@net.bio.net Wed Oct 29 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Elizabeth Ryder Newsgroups: bionet.celegans Subject: making males Date: 29 Oct 1997 18:56:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <638t1a$jpn@net.bio.net> NNTP-Posting-Host: net.bio.net Hi-- We don't seem to be having great success producing males by heat shock, and wonder if anyone has come up with improvements on the heat shocking protocol, or any other ways of producing males. The protocols we have tried are: 1) put L4s/young adults at 30 degrees for 6 hours 2) put L4s/young adults at 27.5 degrees for 30 hours We then move worms back to 20 degrees and look at progeny for males. Does anyone do anything different? Thanks! Liz Liz Ryder Worcester Polytechnic Institute Dept. of Biology and Biotechnology Worcester, MA 01609 email: ryder@wpi.edu phone: 508-831-6011 fax: 508-831-5936 .