From owner-drosophila@net.bio.net Tue Sep 20 23:00:00 1994
Path: biosci!CRCVMS.UNL.EDU!ACHRISTENSEN
From: ACHRISTENSEN@CRCVMS.UNL.EDU ("Alan C. Christensen, Ph.D.")
Newsgroups: bionet.drosophila
Subject: X-gal protocol
Date: 20 Sep 1994 17:33:19 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Hello.  We are getting frustrated with protocols for staining embryos
with X-gal.  We've tried the protocols in Ashburner's Manual, as well
as some other published procedures, and they don't work very 
reliably.  Often the stain is diffuse throughout the embryo rather
than in a pattern, and more often, they don't stain at all, but just
turn light brown.  Could someone please help with a detailed
protocol, as well as some hints on troubleshooting?

Thanks, in advance,

Alan Christensen,  achristensen@unl.edu
School of Biological Sciences
University of Nebraska
348 Manter Hall
Lincoln, NE 68588-0118

.