From owner-celegans@net.bio.net Tue Oct 28 22:00:00 1997
Path: biosci!biosci!not-for-mail
From: "James A. Waddle" <jwaddle@sequencer.wustl.edu>
Newsgroups: bionet.celegans
Subject: Re: Thomas Burglin's need to back cross a vul mutant
Date: 29 Oct 1997 09:05:16 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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>Thomas Burglin writes:
>We have a putative knock-out, but the animals look vulvaless
>and bag.
>We need to backcross this.

Thomas, as an alternative to poking holes in the worm and artificial
insemination, you may, if the mutant is not too sickly, try heat shocking
to increase the frequency of spontaneous males and then mate to N2
hermaphrodites or tra-2(q122) females.

-Jim Waddle



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