From owner-celegans@net.bio.net Sun Jun 07 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: Vic Yuschuk <yuschuv@biomed.med.yale.edu>
Newsgroups: bionet.celegans
Subject: Re: 3D reconstructions
Date: 8 Jun 1998 06:07:08 -0700
Organization: Stern Lab, Genetics Department, Yale University
Lines: 34
Sender: daemon@net.bio.net
Approved: wmorgan@acs.wooster.edu
Distribution: world
Message-ID: <6lgnls$lj4@net.bio.net>
NNTP-Posting-Host: net.bio.net

Patrick Phillips wrote:
>
> Has anyone done or know of 3D worm reconstructions from EM or confocal
> work that show cell locations in three dimensional space? I know of the
> classic EM work, but I don't recall ever hearing if this information has
> ever been digitized. Thanks for your help.
>
> ---------------------------------------------------------------------
> Patrick C. Phillips               pphillips@uta.edu
> Biology Department, Box 19498     http://www.uta.edu/biology/phillips
> University of Texas at Arlington  http://wbar.uta.edu (EPGED)
> Arlington, TX 76019 USA                Tel. (817) 272-2409
> Genes in theory, Worms in practice.    FAX  (817) 272-2855

I've never heard of such reconstructions, but it sure would be great!  The
closest method I've encountered is 4d microscopy.  It appears to be just the
creation of digital movies that can be played forwards and backwards with
variable focal planes.  For more info, you can check out:
http://www.bocklabs.wisc.edu/imr/facility/4D/4d.htm

Vic

               \\|//
               (o o)
-. .-.   .-oOOo~(_)~oOOo-.   .-. .-.   .-. .-.   .-. .-.   .-.
||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X
|/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/
'   `-' `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'
Vic Yuschuk
Stern Lab
Genetics Department
Yale University



.