From owner-celegans@net.bio.net Thu Oct 27 22:00:00 1994
Path: biosci!FRODO.MGH.HARVARD.EDU!BAUMEISTER
From: BAUMEISTER@FRODO.MGH.HARVARD.EDU
Newsgroups: bionet.celegans
Subject: Letter from Morris Maduro, Injection problems: Reply
Date: 28 Oct 1994 05:13:18 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Dear Morris,
1. I do not believe that it is lacZ that causes problems.
2. I have tried several preparation methods for DNA to be injected. 
Minipreps which include and RNase treatment have never worked for me,
omitting the RNase helps. Significantly better is a Qiagen prep (obviously,
you get rid of the RNase on the column) or a CsCl prep. Similar results
were obtained by other people in our lab.
These observations are based on 70+ injections.

Good luck

Ralf Baumeister
Ruvkun lab
Mass. General Hospital 

.